Strain Fitness in Escherichia coli BW25113 around b4042

Experiment: TP11

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntplsB and dgkA are separated by 170 nucleotidesdgkA and lexA are separated by 109 nucleotideslexA and dinF are separated by 18 nucleotides b4041: plsB - glycerol-3-phosphate acyltransferase (VIMSS), at 4,252,066 to 4,254,489 plsB b4042: dgkA - diacylglycerol kinase (NCBI), at 4,254,660 to 4,255,028 dgkA b4043: lexA - LexA repressor (NCBI), at 4,255,138 to 4,255,746 lexA b4044: dinF - DNA-damage-inducible SOS response protein (NCBI), at 4,255,765 to 4,257,144 dinF Position (kb) 4254 4255 4256Strain fitness (log2 ratio) -2 -1 0 1at 4254.489 kb on - strandat 4254.503 kb on - strandat 4254.508 kb on + strandat 4254.508 kb on + strandat 4254.537 kb on - strandat 4254.568 kb on - strandat 4254.588 kb on - strandat 4254.608 kb on + strandat 4254.608 kb on + strandat 4254.618 kb on + strandat 4254.623 kb on + strandat 4254.623 kb on + strandat 4254.626 kb on + strandat 4254.629 kb on - strandat 4254.629 kb on - strandat 4254.681 kb on - strandat 4254.738 kb on - strand, within dgkAat 4254.745 kb on - strand, within dgkAat 4254.745 kb on - strand, within dgkAat 4254.782 kb on - strand, within dgkAat 4254.817 kb on + strand, within dgkAat 4254.824 kb on - strand, within dgkAat 4254.831 kb on + strand, within dgkAat 4254.833 kb on + strand, within dgkAat 4254.870 kb on - strand, within dgkAat 4254.912 kb on + strand, within dgkAat 4255.118 kb on + strandat 4255.916 kb on - strand, within dinF

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Per-strain Table

Position Strand Gene LocusTag Fraction TP11
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4,254,489 - -0.9
4,254,503 - -0.0
4,254,508 + -0.0
4,254,508 + -1.5
4,254,537 - -0.2
4,254,568 - -0.2
4,254,588 - +0.1
4,254,608 + +0.9
4,254,608 + -0.0
4,254,618 + -0.1
4,254,623 + -0.0
4,254,623 + -1.0
4,254,626 + -0.3
4,254,629 - +0.3
4,254,629 - +0.9
4,254,681 - +0.1
4,254,738 - dgkA b4042 0.21 +0.7
4,254,745 - dgkA b4042 0.23 +0.4
4,254,745 - dgkA b4042 0.23 -1.9
4,254,782 - dgkA b4042 0.33 +0.7
4,254,817 + dgkA b4042 0.43 +0.7
4,254,824 - dgkA b4042 0.44 -0.3
4,254,831 + dgkA b4042 0.46 +0.7
4,254,833 + dgkA b4042 0.47 +0.7
4,254,870 - dgkA b4042 0.57 +0.1
4,254,912 + dgkA b4042 0.68 -0.6
4,255,118 + -0.1
4,255,916 - dinF b4044 0.11 -0.6

Or see this region's nucleotide sequence