Strain Fitness in Escherichia coli BW25113 around b1780

Experiment: TP11

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgapA and yeaD are separated by 83 nucleotidesyeaD and yeaE are separated by 47 nucleotidesyeaE and mipA are separated by 89 nucleotides b1779: gapA - glyceraldehyde-3-phosphate dehydrogenase (NCBI), at 1,860,795 to 1,861,790 gapA b1780: yeaD - orf, hypothetical protein (VIMSS), at 1,861,874 to 1,862,758 yeaD b1781: yeaE - predicted oxidoreductase (NCBI), at 1,862,806 to 1,863,660 yeaE b1782: mipA - scaffolding protein for murein synthesizing machinery (NCBI), at 1,863,750 to 1,864,496 mipA Position (kb) 1861 1862 1863Strain fitness (log2 ratio) -2 -1 0 1at 1861.874 kb on - strandat 1861.874 kb on - strandat 1861.922 kb on + strandat 1862.020 kb on - strand, within yeaDat 1862.020 kb on - strand, within yeaDat 1862.069 kb on - strand, within yeaDat 1862.153 kb on + strand, within yeaDat 1862.434 kb on + strand, within yeaDat 1862.442 kb on - strand, within yeaDat 1862.545 kb on + strand, within yeaDat 1862.658 kb on - strand, within yeaDat 1862.691 kb on - strandat 1862.801 kb on + strandat 1862.801 kb on + strandat 1862.851 kb on - strandat 1862.851 kb on - strandat 1862.985 kb on - strand, within yeaEat 1863.088 kb on - strand, within yeaEat 1863.147 kb on + strand, within yeaEat 1863.391 kb on - strand, within yeaEat 1863.454 kb on + strand, within yeaEat 1863.511 kb on + strand, within yeaEat 1863.597 kb on - strandat 1863.603 kb on - strandat 1863.609 kb on - strandat 1863.609 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP11
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1,861,874 - -0.0
1,861,874 - -0.4
1,861,922 + +0.2
1,862,020 - yeaD b1780 0.16 -0.9
1,862,020 - yeaD b1780 0.16 +0.1
1,862,069 - yeaD b1780 0.22 -0.1
1,862,153 + yeaD b1780 0.32 -0.2
1,862,434 + yeaD b1780 0.63 +0.4
1,862,442 - yeaD b1780 0.64 -1.9
1,862,545 + yeaD b1780 0.76 -0.2
1,862,658 - yeaD b1780 0.89 +1.6
1,862,691 - -0.7
1,862,801 + -1.5
1,862,801 + -0.1
1,862,851 - +0.5
1,862,851 - +0.5
1,862,985 - yeaE b1781 0.21 -0.4
1,863,088 - yeaE b1781 0.33 -0.0
1,863,147 + yeaE b1781 0.40 -0.3
1,863,391 - yeaE b1781 0.68 -0.0
1,863,454 + yeaE b1781 0.76 +0.3
1,863,511 + yeaE b1781 0.82 -0.0
1,863,597 - -0.9
1,863,603 - -0.5
1,863,609 - +0.9
1,863,609 - +0.1

Or see this region's nucleotide sequence