Experiment: TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofB and ppdD are separated by 9 nucleotides ppdD and nadC are separated by 202 nucleotides nadC and ampD are separated by 87 nucleotides ampD and ampE overlap by 4 nucleotides
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1 at 116.760 kb on - strand, within hofB at 116.777 kb on + strand, within hofB at 116.777 kb on + strand, within hofB at 116.872 kb on + strand, within hofB at 116.882 kb on + strand, within hofB at 116.957 kb on - strand, within hofB at 116.964 kb on - strand at 117.046 kb on + strand at 117.056 kb on + strand at 117.069 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.081 kb on - strand at 117.081 kb on - strand at 117.084 kb on + strand at 117.087 kb on + strand at 117.227 kb on - strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.293 kb on - strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.385 kb on + strand, within ppdD at 117.387 kb on + strand, within ppdD at 117.508 kb on - strand at 117.508 kb on - strand at 117.511 kb on - strand at 117.511 kb on - strand at 117.525 kb on - strand at 117.525 kb on - strand at 117.603 kb on + strand at 117.603 kb on + strand at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 118.107 kb on - strand, within nadC at 118.201 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.296 kb on + strand, within nadC at 118.355 kb on - strand, within nadC at 118.355 kb on - strand, within nadC at 118.364 kb on + strand, within nadC at 118.382 kb on - strand, within nadC at 118.538 kb on + strand, within nadC at 118.623 kb on + strand at 118.631 kb on - strand at 118.737 kb on + strand at 118.737 kb on + strand at 118.781 kb on + strand at 118.836 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.904 kb on - strand, within ampD at 119.033 kb on - strand, within ampD at 119.101 kb on + strand, within ampD at 119.109 kb on - strand, within ampD at 119.109 kb on - strand, within ampD at 119.117 kb on - strand, within ampD at 119.119 kb on + strand, within ampD at 119.150 kb on - strand, within ampD at 119.150 kb on - strand, within ampD at 119.163 kb on + strand, within ampD at 119.168 kb on - strand, within ampD at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE
Per-strain Table
Position Strand Gene LocusTag Fraction TP9 remove 116,760 - hofB b0107 0.75 +0.1 116,777 + hofB b0107 0.77 +0.9 116,777 + hofB b0107 0.77 +0.9 116,872 + hofB b0107 0.84 +0.1 116,882 + hofB b0107 0.84 +0.2 116,957 - hofB b0107 0.90 -0.3 116,964 - +0.5 117,046 + -0.3 117,056 + -0.0 117,069 + -0.4 117,075 + -1.9 117,075 + +1.2 117,075 + -1.2 117,081 - -0.3 117,081 - +0.5 117,084 + -0.2 117,087 + -0.1 117,227 - ppdD b0108 0.27 +0.4 117,281 + ppdD b0108 0.39 +0.9 117,281 + ppdD b0108 0.39 +1.1 117,293 - ppdD b0108 0.42 -0.0 117,302 + ppdD b0108 0.44 -0.8 117,302 + ppdD b0108 0.44 -0.1 117,385 + ppdD b0108 0.63 -1.6 117,387 + ppdD b0108 0.63 +0.4 117,508 - -0.5 117,508 - +0.7 117,511 - -0.8 117,511 - -0.4 117,525 - +1.5 117,525 - +0.5 117,603 + +0.4 117,603 + -0.0 117,839 + +0.8 117,856 - nadC b0109 0.12 +0.2 117,856 - nadC b0109 0.12 +0.7 117,879 - nadC b0109 0.14 -0.4 117,879 - nadC b0109 0.14 -0.3 117,881 - nadC b0109 0.14 -1.3 117,922 - nadC b0109 0.19 +0.2 117,922 - nadC b0109 0.19 +0.5 118,107 - nadC b0109 0.40 +0.2 118,201 + nadC b0109 0.50 -1.1 118,230 + nadC b0109 0.53 +1.0 118,230 + nadC b0109 0.53 -0.4 118,296 + nadC b0109 0.61 -0.2 118,355 - nadC b0109 0.67 -1.3 118,355 - nadC b0109 0.67 +1.2 118,364 + nadC b0109 0.68 -1.1 118,382 - nadC b0109 0.70 -1.2 118,538 + nadC b0109 0.88 -1.0 118,623 + -0.7 118,631 - -1.9 118,737 + +0.5 118,737 + -0.2 118,781 + +1.4 118,836 + ampD b0110 0.19 -0.0 118,868 + ampD b0110 0.24 -0.7 118,868 + ampD b0110 0.24 -0.1 118,904 - ampD b0110 0.31 -0.3 119,033 - ampD b0110 0.54 +0.1 119,101 + ampD b0110 0.67 -1.5 119,109 - ampD b0110 0.68 -0.0 119,109 - ampD b0110 0.68 -0.4 119,117 - ampD b0110 0.70 -1.6 119,119 + ampD b0110 0.70 +1.2 119,150 - ampD b0110 0.76 +0.1 119,150 - ampD b0110 0.76 -0.1 119,163 + ampD b0110 0.78 +0.9 119,168 - ampD b0110 0.79 +0.3 119,191 + ampD b0110 0.83 +0.3 119,191 + ampD b0110 0.83 -0.4 119,274 + -0.8 119,339 + +0.4 119,339 + -0.1 119,499 - ampE b0111 0.25 -0.8 119,499 - ampE b0111 0.25 +0.3 119,522 + ampE b0111 0.28 -0.3 119,528 - ampE b0111 0.29 -1.0 119,555 - ampE b0111 0.32 +0.3 119,555 - ampE b0111 0.32 -0.1 119,558 - ampE b0111 0.32 +1.5 119,567 + ampE b0111 0.33 +0.4 119,567 + ampE b0111 0.33 +0.5
Or see this region's nucleotide sequence