Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt srlA and srlE overlap by 4 nucleotides srlE and srlB are separated by 10 nucleotides srlB and srlD are separated by 3 nucleotides srlD and gutM are separated by 104 nucleotides
b2702: srlA - glucitol/sorbitol-specific enzyme IIC component of PTS (NCBI), at 2,823,854 to 2,824,417
srlA
b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373
srlE
b2704: srlB - glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI), at 2,825,384 to 2,825,755
srlB
b2705: srlD - 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI), at 2,825,759 to 2,826,538
srlD
b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002
gutM
Position (kb)
2825
2826 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2824.412 kb on - strand at 2824.412 kb on - strand at 2824.460 kb on + strand at 2824.683 kb on - strand, within srlE at 2824.736 kb on - strand, within srlE at 2824.775 kb on + strand, within srlE at 2824.933 kb on - strand, within srlE at 2824.938 kb on - strand, within srlE at 2824.938 kb on - strand, within srlE at 2824.969 kb on + strand, within srlE at 2824.969 kb on + strand, within srlE at 2824.983 kb on + strand, within srlE at 2824.983 kb on + strand, within srlE at 2825.009 kb on + strand, within srlE at 2825.113 kb on - strand, within srlE at 2825.179 kb on + strand, within srlE at 2825.263 kb on + strand, within srlE at 2825.263 kb on + strand, within srlE at 2825.296 kb on + strand at 2825.569 kb on + strand, within srlB at 2825.599 kb on - strand, within srlB at 2825.631 kb on + strand, within srlB at 2825.631 kb on + strand, within srlB at 2825.641 kb on + strand, within srlB at 2825.689 kb on - strand, within srlB at 2825.709 kb on + strand, within srlB at 2825.734 kb on + strand at 2825.807 kb on + strand at 2825.921 kb on - strand, within srlD at 2825.949 kb on - strand, within srlD at 2825.949 kb on - strand, within srlD at 2825.973 kb on + strand, within srlD at 2826.044 kb on - strand, within srlD at 2826.210 kb on + strand, within srlD at 2826.210 kb on + strand, within srlD at 2826.215 kb on + strand, within srlD at 2826.218 kb on - strand, within srlD at 2826.218 kb on - strand, within srlD at 2826.245 kb on + strand, within srlD at 2826.318 kb on - strand, within srlD at 2826.397 kb on + strand, within srlD at 2826.397 kb on + strand, within srlD at 2826.397 kb on + strand, within srlD at 2826.397 kb on + strand, within srlD at 2826.474 kb on + strand at 2826.474 kb on + strand at 2826.482 kb on - strand at 2826.490 kb on - strand at 2826.490 kb on - strand at 2826.613 kb on - strand at 2826.613 kb on - strand at 2826.613 kb on - strand at 2826.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,824,412 - -1.0 2,824,412 - +0.8 2,824,460 + -1.0 2,824,683 - srlE b2703 0.28 -1.9 2,824,736 - srlE b2703 0.34 -2.5 2,824,775 + srlE b2703 0.38 +0.9 2,824,933 - srlE b2703 0.54 -2.7 2,824,938 - srlE b2703 0.55 +0.2 2,824,938 - srlE b2703 0.55 -0.7 2,824,969 + srlE b2703 0.58 -0.9 2,824,969 + srlE b2703 0.58 -0.5 2,824,983 + srlE b2703 0.59 -0.0 2,824,983 + srlE b2703 0.59 +0.8 2,825,009 + srlE b2703 0.62 -1.3 2,825,113 - srlE b2703 0.73 -0.1 2,825,179 + srlE b2703 0.80 -0.3 2,825,263 + srlE b2703 0.88 +1.0 2,825,263 + srlE b2703 0.88 -1.2 2,825,296 + -1.1 2,825,569 + srlB b2704 0.50 -1.1 2,825,599 - srlB b2704 0.58 -2.4 2,825,631 + srlB b2704 0.66 +0.8 2,825,631 + srlB b2704 0.66 +0.2 2,825,641 + srlB b2704 0.69 -3.9 2,825,689 - srlB b2704 0.82 -1.1 2,825,709 + srlB b2704 0.87 +0.7 2,825,734 + -0.8 2,825,807 + -1.3 2,825,921 - srlD b2705 0.21 -1.1 2,825,949 - srlD b2705 0.24 -0.9 2,825,949 - srlD b2705 0.24 -2.1 2,825,973 + srlD b2705 0.27 -1.6 2,826,044 - srlD b2705 0.37 -1.8 2,826,210 + srlD b2705 0.58 +0.3 2,826,210 + srlD b2705 0.58 +0.2 2,826,215 + srlD b2705 0.58 -0.6 2,826,218 - srlD b2705 0.59 -1.3 2,826,218 - srlD b2705 0.59 +0.5 2,826,245 + srlD b2705 0.62 +1.1 2,826,318 - srlD b2705 0.72 +0.0 2,826,397 + srlD b2705 0.82 -2.0 2,826,397 + srlD b2705 0.82 -0.9 2,826,397 + srlD b2705 0.82 -1.1 2,826,397 + srlD b2705 0.82 -0.4 2,826,474 + +0.3 2,826,474 + -0.0 2,826,482 - -1.3 2,826,490 - +0.7 2,826,490 - -0.0 2,826,613 - -0.3 2,826,613 - +0.6 2,826,613 - +0.8 2,826,646 - -3.7
Or see this region's nucleotide sequence