Strain Fitness in Escherichia coli BW25113 around b1608

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfolM and ydgC overlap by 4 nucleotidesydgC and rstA are separated by 128 nucleotidesrstA and rstB are separated by 3 nucleotides b1606: folM - short chain dehydrogenase (NCBI), at 1,679,000 to 1,679,722 folM b1607: ydgC - conserved inner membrane protein associated with alginate biosynthesis (NCBI), at 1,679,719 to 1,680,054 ydgC b1608: rstA - DNA-binding response regulator in two-component regulatory system with RstB (RefSeq), at 1,680,183 to 1,680,902 rstA b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207 rstB Position (kb) 1680 1681Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1679.198 kb on - strand, within folMat 1679.226 kb on + strand, within folMat 1679.328 kb on - strand, within folMat 1679.336 kb on - strand, within folMat 1679.342 kb on + strand, within folMat 1679.342 kb on - strand, within folMat 1679.342 kb on - strand, within folMat 1679.342 kb on - strand, within folMat 1679.349 kb on + strand, within folMat 1679.376 kb on + strand, within folMat 1679.390 kb on + strand, within folMat 1679.484 kb on - strand, within folMat 1679.538 kb on + strand, within folMat 1679.538 kb on + strand, within folMat 1679.887 kb on + strand, within ydgCat 1679.897 kb on + strand, within ydgCat 1679.924 kb on - strand, within ydgCat 1680.147 kb on + strandat 1680.268 kb on + strand, within rstAat 1680.438 kb on + strand, within rstAat 1680.439 kb on + strand, within rstAat 1680.439 kb on + strand, within rstAat 1680.544 kb on + strand, within rstAat 1680.552 kb on - strand, within rstAat 1680.552 kb on - strand, within rstAat 1680.575 kb on + strand, within rstAat 1680.981 kb on + strandat 1680.981 kb on + strandat 1681.080 kb on + strand, within rstBat 1681.099 kb on - strand, within rstBat 1681.176 kb on + strand, within rstBat 1681.555 kb on + strand, within rstBat 1681.555 kb on + strand, within rstBat 1681.779 kb on - strand, within rstBat 1681.779 kb on - strand, within rstBat 1681.827 kb on + strand, within rstB

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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1,679,198 - folM b1606 0.27 -0.1
1,679,226 + folM b1606 0.31 -0.9
1,679,328 - folM b1606 0.45 -0.1
1,679,336 - folM b1606 0.46 +2.8
1,679,342 + folM b1606 0.47 -0.8
1,679,342 - folM b1606 0.47 +0.4
1,679,342 - folM b1606 0.47 -1.4
1,679,342 - folM b1606 0.47 -2.1
1,679,349 + folM b1606 0.48 +0.1
1,679,376 + folM b1606 0.52 +0.1
1,679,390 + folM b1606 0.54 -3.3
1,679,484 - folM b1606 0.67 +0.1
1,679,538 + folM b1606 0.74 +0.8
1,679,538 + folM b1606 0.74 -0.8
1,679,887 + ydgC b1607 0.50 -1.2
1,679,897 + ydgC b1607 0.53 -1.8
1,679,924 - ydgC b1607 0.61 -0.4
1,680,147 + -0.2
1,680,268 + rstA b1608 0.12 +0.2
1,680,438 + rstA b1608 0.35 +0.6
1,680,439 + rstA b1608 0.36 -0.3
1,680,439 + rstA b1608 0.36 -1.2
1,680,544 + rstA b1608 0.50 -0.6
1,680,552 - rstA b1608 0.51 +0.2
1,680,552 - rstA b1608 0.51 -0.1
1,680,575 + rstA b1608 0.54 +1.2
1,680,981 + -0.0
1,680,981 + -0.3
1,681,080 + rstB b1609 0.13 -0.7
1,681,099 - rstB b1609 0.15 -0.3
1,681,176 + rstB b1609 0.21 -0.6
1,681,555 + rstB b1609 0.50 -0.5
1,681,555 + rstB b1609 0.50 +0.0
1,681,779 - rstB b1609 0.67 -0.2
1,681,779 - rstB b1609 0.67 +0.0
1,681,827 + rstB b1609 0.71 +1.0

Or see this region's nucleotide sequence