Strain Fitness in Escherichia coli BW25113 around b0684

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybfN and fur are separated by 83 nucleotidesfur and uof overlap by 8 nucleotidesuof and fldA are separated by 209 nucleotidesfldA and ybfE are separated by 139 nucleotidesybfE and ybfF are separated by 139 nucleotides b0682: ybfN - predicted lipoprotein (NCBI), at 709,013 to 709,339 ybfN b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869 fur b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948 uof b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688 fldA b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121 ybfE b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF Position (kb) 710 711Strain fitness (log2 ratio) -1 0 1 2at 709.238 kb on + strand, within ybfNat 709.247 kb on - strand, within ybfNat 709.247 kb on - strand, within ybfNat 709.257 kb on - strand, within ybfNat 709.428 kb on - strandat 709.428 kb on - strandat 709.595 kb on + strand, within furat 709.678 kb on - strand, within furat 709.699 kb on + strand, within furat 709.861 kb on - strandat 710.067 kb on - strandat 710.080 kb on + strandat 710.148 kb on - strandat 710.148 kb on - strandat 710.163 kb on - strandat 710.751 kb on + strandat 710.780 kb on - strandat 710.780 kb on - strandat 710.787 kb on + strandat 710.798 kb on + strandat 710.805 kb on - strandat 710.805 kb on - strandat 710.807 kb on + strandat 710.901 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 710.924 kb on + strand, within ybfEat 711.159 kb on - strandat 711.400 kb on - strand, within ybfFat 711.569 kb on + strand, within ybfFat 711.668 kb on - strand, within ybfFat 711.685 kb on + strand, within ybfF

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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709,238 + ybfN b0682 0.69 -0.6
709,247 - ybfN b0682 0.72 +0.1
709,247 - ybfN b0682 0.72 +1.1
709,257 - ybfN b0682 0.75 -0.7
709,428 - +0.3
709,428 - +0.7
709,595 + fur b0683 0.38 +1.4
709,678 - fur b0683 0.57 +2.0
709,699 + fur b0683 0.62 +2.0
709,861 - +1.6
710,067 - +1.5
710,080 + -1.2
710,148 - +1.1
710,148 - -0.5
710,163 - +1.2
710,751 + -0.3
710,780 - +1.0
710,780 - +0.5
710,787 + -0.5
710,798 + -0.4
710,805 - +1.0
710,805 - -0.3
710,807 + +0.3
710,901 + ybfE b0685 0.25 -1.4
710,924 + ybfE b0685 0.33 -0.0
710,924 + ybfE b0685 0.33 -1.1
711,159 - +1.1
711,400 - ybfF b0686 0.18 +0.1
711,569 + ybfF b0686 0.40 -0.0
711,668 - ybfF b0686 0.53 -1.3
711,685 + ybfF b0686 0.55 -0.3

Or see this region's nucleotide sequence