Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purC and nlpB are separated by 212 nucleotides nlpB and dapA are separated by 16 nucleotides
b2476: purC - phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI), at 2,594,927 to 2,595,640
purC
b2477: nlpB - lipoprotein-34 (VIMSS), at 2,595,853 to 2,596,887
nlpB
b2478: dapA - dihydrodipicolinate synthase (NCBI), at 2,596,904 to 2,597,782
dapA
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2594.872 kb on - strand at 2594.943 kb on - strand at 2594.980 kb on + strand at 2595.179 kb on - strand at 2595.179 kb on - strand, within purC at 2595.364 kb on - strand, within purC at 2595.494 kb on + strand, within purC at 2595.574 kb on + strand at 2595.593 kb on + strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.625 kb on - strand at 2595.837 kb on + strand at 2595.877 kb on - strand at 2595.937 kb on + strand at 2595.937 kb on + strand at 2595.944 kb on - strand at 2595.944 kb on - strand at 2595.991 kb on + strand, within nlpB at 2595.991 kb on + strand, within nlpB at 2596.206 kb on + strand, within nlpB at 2596.295 kb on - strand, within nlpB at 2596.349 kb on - strand, within nlpB at 2596.439 kb on - strand, within nlpB at 2596.441 kb on + strand, within nlpB at 2596.792 kb on + strand at 2596.792 kb on + strand at 2596.883 kb on - strand at 2596.883 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,594,872 - +0.7 2,594,943 - -0.6 2,594,980 + -0.4 2,595,179 - +1.8 2,595,179 - purC b2476 0.35 -0.4 2,595,364 - purC b2476 0.61 +0.0 2,595,494 + purC b2476 0.79 +1.1 2,595,574 + -0.4 2,595,593 + -0.1 2,595,601 - -1.0 2,595,601 - +0.3 2,595,601 - -1.5 2,595,601 - -0.8 2,595,601 - +0.3 2,595,625 - +1.6 2,595,837 + +0.5 2,595,877 - +1.2 2,595,937 + +0.6 2,595,937 + +0.3 2,595,944 - +0.2 2,595,944 - +2.2 2,595,991 + nlpB b2477 0.13 +0.9 2,595,991 + nlpB b2477 0.13 +1.0 2,596,206 + nlpB b2477 0.34 +0.4 2,596,295 - nlpB b2477 0.43 +1.6 2,596,349 - nlpB b2477 0.48 +0.8 2,596,439 - nlpB b2477 0.57 +1.2 2,596,441 + nlpB b2477 0.57 +1.0 2,596,792 + +3.2 2,596,792 + +2.2 2,596,883 - +0.6 2,596,883 - +0.3
Or see this region's nucleotide sequence