Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehS and yehT are separated by 46 nucleotides yehT and yehU overlap by 4 nucleotides yehU and mlrA are separated by 221 nucleotides
b2124: yehS - hypothetical protein (NCBI), at 2,209,748 to 2,210,218
yehS
b2125: yehT - orf, hypothetical protein (VIMSS), at 2,210,265 to 2,210,984
yehT
b2126: yehU - predicted sensory kinase in two-component system with YehT (NCBI), at 2,210,981 to 2,212,666
yehU
b2127: mlrA - DNA-binding transcriptional regulator (NCBI), at 2,212,888 to 2,213,619
mlrA
Position (kb)
2210
2211
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1 at 2210.041 kb on - strand, within yehS at 2210.106 kb on - strand, within yehS at 2210.106 kb on - strand, within yehS at 2210.138 kb on - strand, within yehS at 2210.161 kb on + strand, within yehS at 2210.185 kb on + strand at 2210.193 kb on - strand at 2210.203 kb on - strand at 2210.313 kb on - strand at 2210.408 kb on - strand, within yehT at 2210.411 kb on - strand, within yehT at 2210.492 kb on + strand, within yehT at 2210.492 kb on + strand, within yehT at 2210.498 kb on - strand, within yehT at 2210.502 kb on + strand, within yehT at 2210.695 kb on - strand, within yehT at 2210.695 kb on - strand, within yehT at 2210.715 kb on + strand, within yehT at 2210.715 kb on + strand, within yehT at 2210.858 kb on - strand, within yehT at 2210.858 kb on - strand, within yehT at 2210.936 kb on + strand at 2210.954 kb on - strand at 2210.962 kb on - strand at 2210.962 kb on - strand at 2211.049 kb on - strand at 2211.051 kb on + strand at 2211.130 kb on - strand at 2211.130 kb on - strand at 2211.207 kb on - strand, within yehU at 2211.207 kb on - strand, within yehU at 2211.218 kb on - strand, within yehU at 2211.273 kb on - strand, within yehU at 2211.273 kb on - strand, within yehU at 2211.371 kb on - strand, within yehU at 2211.399 kb on - strand, within yehU at 2211.451 kb on - strand, within yehU at 2211.464 kb on - strand, within yehU at 2211.539 kb on - strand, within yehU at 2211.539 kb on - strand, within yehU at 2211.651 kb on + strand, within yehU at 2211.675 kb on + strand, within yehU at 2211.688 kb on - strand, within yehU at 2211.688 kb on - strand, within yehU at 2211.782 kb on - strand, within yehU at 2211.806 kb on - strand, within yehU at 2211.826 kb on + strand, within yehU at 2211.834 kb on - strand, within yehU at 2211.961 kb on - strand, within yehU at 2211.972 kb on - strand, within yehU at 2211.972 kb on - strand, within yehU at 2212.028 kb on + strand, within yehU at 2212.168 kb on + strand, within yehU at 2212.177 kb on - strand, within yehU at 2212.177 kb on - strand, within yehU at 2212.256 kb on - strand, within yehU at 2212.264 kb on - strand, within yehU at 2212.294 kb on + strand, within yehU at 2212.358 kb on - strand, within yehU at 2212.358 kb on - strand, within yehU at 2212.365 kb on + strand, within yehU at 2212.373 kb on - strand, within yehU at 2212.438 kb on - strand, within yehU at 2212.438 kb on - strand, within yehU at 2212.564 kb on + strand at 2213.000 kb on + strand, within mlrA at 2213.008 kb on - strand, within mlrA at 2213.008 kb on - strand, within mlrA at 2213.066 kb on - strand, within mlrA at 2213.222 kb on - strand, within mlrA at 2213.244 kb on + strand, within mlrA at 2213.370 kb on + strand, within mlrA at 2213.370 kb on + strand, within mlrA at 2213.444 kb on + strand, within mlrA at 2213.484 kb on + strand, within mlrA at 2213.506 kb on + strand, within mlrA at 2213.506 kb on + strand, within mlrA at 2213.513 kb on + strand, within mlrA at 2213.641 kb on + strand at 2213.641 kb on + strand at 2213.646 kb on + strand at 2213.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,210,041 - yehS b2124 0.62 -0.3 2,210,106 - yehS b2124 0.76 +1.1 2,210,106 - yehS b2124 0.76 +1.6 2,210,138 - yehS b2124 0.83 -1.1 2,210,161 + yehS b2124 0.88 +0.9 2,210,185 + -0.5 2,210,193 - -1.2 2,210,203 - -0.5 2,210,313 - -1.8 2,210,408 - yehT b2125 0.20 -0.2 2,210,411 - yehT b2125 0.20 -0.9 2,210,492 + yehT b2125 0.32 +0.0 2,210,492 + yehT b2125 0.32 +0.2 2,210,498 - yehT b2125 0.32 -1.3 2,210,502 + yehT b2125 0.33 +1.0 2,210,695 - yehT b2125 0.60 +1.0 2,210,695 - yehT b2125 0.60 -0.8 2,210,715 + yehT b2125 0.62 -1.0 2,210,715 + yehT b2125 0.62 +0.4 2,210,858 - yehT b2125 0.82 -0.7 2,210,858 - yehT b2125 0.82 -1.3 2,210,936 + -2.2 2,210,954 - +1.0 2,210,962 - -0.6 2,210,962 - -1.8 2,211,049 - +0.5 2,211,051 + -0.8 2,211,130 - -1.4 2,211,130 - -0.5 2,211,207 - yehU b2126 0.13 -1.0 2,211,207 - yehU b2126 0.13 -0.3 2,211,218 - yehU b2126 0.14 -0.9 2,211,273 - yehU b2126 0.17 -2.3 2,211,273 - yehU b2126 0.17 -0.6 2,211,371 - yehU b2126 0.23 +0.3 2,211,399 - yehU b2126 0.25 +0.2 2,211,451 - yehU b2126 0.28 -1.1 2,211,464 - yehU b2126 0.29 +0.2 2,211,539 - yehU b2126 0.33 +0.9 2,211,539 - yehU b2126 0.33 +1.2 2,211,651 + yehU b2126 0.40 -2.1 2,211,675 + yehU b2126 0.41 -1.9 2,211,688 - yehU b2126 0.42 -0.2 2,211,688 - yehU b2126 0.42 -0.1 2,211,782 - yehU b2126 0.48 -1.1 2,211,806 - yehU b2126 0.49 -0.6 2,211,826 + yehU b2126 0.50 -1.1 2,211,834 - yehU b2126 0.51 +0.4 2,211,961 - yehU b2126 0.58 -1.4 2,211,972 - yehU b2126 0.59 -0.6 2,211,972 - yehU b2126 0.59 -2.2 2,212,028 + yehU b2126 0.62 -1.1 2,212,168 + yehU b2126 0.70 -1.4 2,212,177 - yehU b2126 0.71 +0.4 2,212,177 - yehU b2126 0.71 -2.1 2,212,256 - yehU b2126 0.76 -1.9 2,212,264 - yehU b2126 0.76 -2.2 2,212,294 + yehU b2126 0.78 -1.0 2,212,358 - yehU b2126 0.82 -1.3 2,212,358 - yehU b2126 0.82 -1.1 2,212,365 + yehU b2126 0.82 -0.4 2,212,373 - yehU b2126 0.83 -2.0 2,212,438 - yehU b2126 0.86 +0.4 2,212,438 - yehU b2126 0.86 -2.6 2,212,564 + +0.6 2,213,000 + mlrA b2127 0.15 -1.6 2,213,008 - mlrA b2127 0.16 -0.8 2,213,008 - mlrA b2127 0.16 +0.6 2,213,066 - mlrA b2127 0.24 -0.8 2,213,222 - mlrA b2127 0.46 +1.6 2,213,244 + mlrA b2127 0.49 -1.0 2,213,370 + mlrA b2127 0.66 +0.2 2,213,370 + mlrA b2127 0.66 -1.1 2,213,444 + mlrA b2127 0.76 -0.7 2,213,484 + mlrA b2127 0.81 -0.5 2,213,506 + mlrA b2127 0.84 +0.1 2,213,506 + mlrA b2127 0.84 +0.7 2,213,513 + mlrA b2127 0.85 +0.0 2,213,641 + +0.2 2,213,641 + -1.3 2,213,646 + +0.1 2,213,654 - +0.1
Or see this region's nucleotide sequence