Strain Fitness in Escherichia coli BW25113 around b4106

Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnD and phnC are separated by 24 nucleotidesphnC and phnB are separated by 132 nucleotides b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375 phnD b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188 phnC b4107: phnB - hypothetical protein (NCBI), at 4,323,321 to 4,323,764 phnB Position (kb) 4322 4323 4324Strain fitness (log2 ratio) -1 0 1at 4321.421 kb on - strandat 4321.440 kb on - strandat 4321.440 kb on - strandat 4321.512 kb on - strand, within phnDat 4321.572 kb on + strand, within phnDat 4321.637 kb on - strand, within phnDat 4321.637 kb on - strand, within phnDat 4321.713 kb on - strand, within phnDat 4321.713 kb on - strand, within phnDat 4321.716 kb on + strand, within phnDat 4321.834 kb on + strand, within phnDat 4321.834 kb on + strand, within phnDat 4321.915 kb on - strand, within phnDat 4322.004 kb on + strand, within phnDat 4322.088 kb on - strand, within phnDat 4322.155 kb on + strand, within phnDat 4322.155 kb on + strand, within phnDat 4322.249 kb on - strand, within phnDat 4322.310 kb on + strandat 4322.328 kb on + strandat 4322.335 kb on - strandat 4322.415 kb on + strandat 4322.415 kb on + strandat 4322.429 kb on + strandat 4322.463 kb on + strandat 4322.471 kb on + strandat 4322.471 kb on + strandat 4322.525 kb on + strand, within phnCat 4322.538 kb on - strand, within phnCat 4322.563 kb on + strand, within phnCat 4322.563 kb on + strand, within phnCat 4322.841 kb on - strand, within phnCat 4322.856 kb on - strand, within phnCat 4322.915 kb on + strand, within phnCat 4322.915 kb on + strand, within phnCat 4322.963 kb on - strand, within phnCat 4322.988 kb on - strand, within phnCat 4323.255 kb on + strandat 4323.352 kb on + strandat 4323.369 kb on + strand, within phnBat 4323.369 kb on + strand, within phnBat 4323.387 kb on + strand, within phnBat 4323.500 kb on + strand, within phnBat 4323.544 kb on - strand, within phnBat 4323.556 kb on - strand, within phnBat 4323.556 kb on - strand, within phnBat 4323.568 kb on + strand, within phnBat 4323.613 kb on + strand, within phnBat 4323.686 kb on - strand, within phnBat 4323.686 kb on - strand, within phnBat 4323.686 kb on - strand, within phnBat 4323.769 kb on - strandat 4323.769 kb on - strandat 4323.776 kb on + strandat 4323.826 kb on - strandat 4323.860 kb on - strandat 4324.091 kb on + strandat 4324.091 kb on + strandat 4324.101 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
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4,321,421 - -0.4
4,321,440 - -0.4
4,321,440 - -0.1
4,321,512 - phnD b4105 0.15 -0.7
4,321,572 + phnD b4105 0.21 +0.6
4,321,637 - phnD b4105 0.27 +0.2
4,321,637 - phnD b4105 0.27 +0.9
4,321,713 - phnD b4105 0.35 +0.1
4,321,713 - phnD b4105 0.35 -0.2
4,321,716 + phnD b4105 0.35 -0.3
4,321,834 + phnD b4105 0.47 +0.7
4,321,834 + phnD b4105 0.47 +0.3
4,321,915 - phnD b4105 0.55 +0.6
4,322,004 + phnD b4105 0.63 -0.1
4,322,088 - phnD b4105 0.72 -0.2
4,322,155 + phnD b4105 0.78 +0.2
4,322,155 + phnD b4105 0.78 -0.1
4,322,249 - phnD b4105 0.88 -0.7
4,322,310 + +0.5
4,322,328 + +0.8
4,322,335 - -0.2
4,322,415 + +0.3
4,322,415 + -0.1
4,322,429 + +0.5
4,322,463 + -0.3
4,322,471 + +0.2
4,322,471 + -0.3
4,322,525 + phnC b4106 0.16 -0.5
4,322,538 - phnC b4106 0.17 -0.3
4,322,563 + phnC b4106 0.21 +0.1
4,322,563 + phnC b4106 0.21 +0.5
4,322,841 - phnC b4106 0.56 -0.9
4,322,856 - phnC b4106 0.58 -0.5
4,322,915 + phnC b4106 0.65 +0.7
4,322,915 + phnC b4106 0.65 +0.4
4,322,963 - phnC b4106 0.71 +0.3
4,322,988 - phnC b4106 0.75 -0.0
4,323,255 + +0.5
4,323,352 + +0.7
4,323,369 + phnB b4107 0.11 -0.1
4,323,369 + phnB b4107 0.11 -0.1
4,323,387 + phnB b4107 0.15 +0.1
4,323,500 + phnB b4107 0.40 -0.4
4,323,544 - phnB b4107 0.50 +1.0
4,323,556 - phnB b4107 0.53 -0.8
4,323,556 - phnB b4107 0.53 -0.2
4,323,568 + phnB b4107 0.56 +0.0
4,323,613 + phnB b4107 0.66 +0.2
4,323,686 - phnB b4107 0.82 +0.3
4,323,686 - phnB b4107 0.82 +0.1
4,323,686 - phnB b4107 0.82 +1.0
4,323,769 - +1.0
4,323,769 - -0.6
4,323,776 + +0.4
4,323,826 - -0.2
4,323,860 - -0.2
4,324,091 + +0.8
4,324,091 + +0.7
4,324,101 + -0.6

Or see this region's nucleotide sequence