Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsB and rbsK are separated by 125 nucleotides rbsK and rbsR are separated by 3 nucleotides rbsR and hsrA overlap by 35 nucleotides
b3751: rbsB - D-ribose transporter subunit (NCBI), at 3,934,301 to 3,935,191
rbsB
b3752: rbsK - ribokinase (NCBI), at 3,935,317 to 3,936,246
rbsK
b3753: rbsR - DNA-binding transcriptional repressor of ribose metabolism (NCBI), at 3,936,250 to 3,937,242
rbsR
b3754: hsrA - predicted multidrug or homocysteine efflux system (NCBI), at 3,937,208 to 3,938,635
hsrA
Position (kb)
3935
3936
3937 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 3934.323 kb on + strand at 3934.323 kb on + strand at 3934.337 kb on + strand at 3934.383 kb on + strand at 3934.397 kb on - strand, within rbsB at 3934.467 kb on + strand, within rbsB at 3934.478 kb on + strand, within rbsB at 3934.489 kb on - strand, within rbsB at 3934.490 kb on + strand, within rbsB at 3934.528 kb on + strand, within rbsB at 3934.594 kb on - strand, within rbsB at 3934.594 kb on - strand, within rbsB at 3934.643 kb on - strand, within rbsB at 3934.684 kb on + strand, within rbsB at 3934.684 kb on + strand, within rbsB at 3934.687 kb on - strand, within rbsB at 3934.698 kb on - strand, within rbsB at 3934.698 kb on - strand, within rbsB at 3934.714 kb on + strand, within rbsB at 3934.714 kb on + strand, within rbsB at 3934.722 kb on - strand, within rbsB at 3934.751 kb on - strand, within rbsB at 3934.767 kb on + strand, within rbsB at 3934.774 kb on + strand, within rbsB at 3934.781 kb on + strand, within rbsB at 3934.842 kb on - strand, within rbsB at 3934.873 kb on + strand, within rbsB at 3934.881 kb on - strand, within rbsB at 3934.881 kb on - strand, within rbsB at 3934.908 kb on - strand, within rbsB at 3934.927 kb on + strand, within rbsB at 3934.930 kb on - strand, within rbsB at 3934.930 kb on - strand, within rbsB at 3934.931 kb on + strand, within rbsB at 3934.940 kb on - strand, within rbsB at 3934.950 kb on - strand, within rbsB at 3935.013 kb on + strand, within rbsB at 3935.022 kb on + strand, within rbsB at 3935.144 kb on + strand at 3935.155 kb on + strand at 3935.163 kb on - strand at 3935.201 kb on + strand at 3935.212 kb on - strand at 3935.222 kb on + strand at 3935.280 kb on + strand at 3935.420 kb on + strand, within rbsK at 3935.420 kb on + strand, within rbsK at 3935.469 kb on - strand, within rbsK at 3935.485 kb on - strand, within rbsK at 3935.493 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.560 kb on - strand, within rbsK at 3935.577 kb on - strand, within rbsK at 3935.650 kb on + strand, within rbsK at 3935.656 kb on - strand, within rbsK at 3935.661 kb on + strand, within rbsK at 3935.666 kb on + strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.711 kb on + strand, within rbsK at 3935.742 kb on + strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.808 kb on + strand, within rbsK at 3935.942 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.955 kb on - strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.983 kb on + strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.009 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.048 kb on + strand, within rbsK at 3936.055 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.082 kb on + strand, within rbsK at 3936.082 kb on + strand, within rbsK at 3936.197 kb on + strand at 3936.338 kb on - strand at 3936.338 kb on - strand at 3936.395 kb on + strand, within rbsR at 3936.407 kb on + strand, within rbsR at 3936.415 kb on - strand, within rbsR at 3936.444 kb on - strand, within rbsR at 3936.447 kb on - strand, within rbsR at 3936.456 kb on + strand, within rbsR at 3936.456 kb on + strand, within rbsR at 3936.463 kb on - strand, within rbsR at 3936.470 kb on + strand, within rbsR at 3936.470 kb on + strand, within rbsR at 3936.473 kb on - strand, within rbsR at 3936.473 kb on - strand, within rbsR at 3936.496 kb on - strand, within rbsR at 3936.496 kb on - strand, within rbsR at 3936.545 kb on + strand, within rbsR at 3936.659 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.684 kb on - strand, within rbsR at 3936.684 kb on - strand, within rbsR at 3936.686 kb on + strand, within rbsR at 3936.723 kb on - strand, within rbsR at 3936.726 kb on - strand, within rbsR at 3936.763 kb on - strand, within rbsR at 3936.769 kb on + strand, within rbsR at 3936.794 kb on + strand, within rbsR at 3936.842 kb on - strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.875 kb on + strand, within rbsR at 3936.876 kb on - strand, within rbsR at 3936.876 kb on - strand, within rbsR at 3936.878 kb on + strand, within rbsR at 3936.878 kb on + strand, within rbsR at 3936.943 kb on + strand, within rbsR at 3936.953 kb on - strand, within rbsR at 3936.953 kb on - strand, within rbsR at 3936.989 kb on + strand, within rbsR at 3936.989 kb on + strand, within rbsR at 3936.990 kb on + strand, within rbsR at 3936.992 kb on + strand, within rbsR at 3937.039 kb on + strand, within rbsR at 3937.055 kb on - strand, within rbsR at 3937.101 kb on + strand, within rbsR at 3937.124 kb on - strand, within rbsR at 3937.124 kb on - strand, within rbsR at 3937.130 kb on - strand, within rbsR at 3937.137 kb on + strand, within rbsR at 3937.174 kb on + strand at 3937.174 kb on + strand at 3937.177 kb on - strand at 3937.184 kb on - strand at 3937.186 kb on + strand at 3937.186 kb on + strand at 3937.201 kb on + strand at 3937.205 kb on + strand at 3937.244 kb on - strand at 3937.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) remove 3,934,323 + -0.6 3,934,323 + -0.8 3,934,337 + -1.5 3,934,383 + -1.0 3,934,397 - rbsB b3751 0.11 +0.7 3,934,467 + rbsB b3751 0.19 -1.5 3,934,478 + rbsB b3751 0.20 -1.4 3,934,489 - rbsB b3751 0.21 +1.6 3,934,490 + rbsB b3751 0.21 -1.5 3,934,528 + rbsB b3751 0.25 -1.6 3,934,594 - rbsB b3751 0.33 +0.5 3,934,594 - rbsB b3751 0.33 -0.0 3,934,643 - rbsB b3751 0.38 -0.1 3,934,684 + rbsB b3751 0.43 -1.9 3,934,684 + rbsB b3751 0.43 +0.4 3,934,687 - rbsB b3751 0.43 -0.1 3,934,698 - rbsB b3751 0.45 +1.2 3,934,698 - rbsB b3751 0.45 +0.8 3,934,714 + rbsB b3751 0.46 -1.3 3,934,714 + rbsB b3751 0.46 -1.9 3,934,722 - rbsB b3751 0.47 +0.3 3,934,751 - rbsB b3751 0.51 +0.6 3,934,767 + rbsB b3751 0.52 -0.6 3,934,774 + rbsB b3751 0.53 -0.8 3,934,781 + rbsB b3751 0.54 -0.2 3,934,842 - rbsB b3751 0.61 -0.1 3,934,873 + rbsB b3751 0.64 -1.2 3,934,881 - rbsB b3751 0.65 +1.0 3,934,881 - rbsB b3751 0.65 +1.3 3,934,908 - rbsB b3751 0.68 +1.8 3,934,927 + rbsB b3751 0.70 -0.1 3,934,930 - rbsB b3751 0.71 +1.1 3,934,930 - rbsB b3751 0.71 +1.8 3,934,931 + rbsB b3751 0.71 -1.0 3,934,940 - rbsB b3751 0.72 +1.3 3,934,950 - rbsB b3751 0.73 +0.7 3,935,013 + rbsB b3751 0.80 -1.6 3,935,022 + rbsB b3751 0.81 +0.1 3,935,144 + -2.8 3,935,155 + -2.0 3,935,163 - +5.5 3,935,201 + -0.2 3,935,212 - +3.2 3,935,222 + +1.2 3,935,280 + -1.1 3,935,420 + rbsK b3752 0.11 +3.2 3,935,420 + rbsK b3752 0.11 +4.3 3,935,469 - rbsK b3752 0.16 +5.3 3,935,485 - rbsK b3752 0.18 +4.7 3,935,493 - rbsK b3752 0.19 +4.4 3,935,517 - rbsK b3752 0.22 +4.8 3,935,517 - rbsK b3752 0.22 +5.8 3,935,519 + rbsK b3752 0.22 +2.9 3,935,519 + rbsK b3752 0.22 +3.2 3,935,522 - rbsK b3752 0.22 +5.4 3,935,522 - rbsK b3752 0.22 +5.2 3,935,542 - rbsK b3752 0.24 +5.8 3,935,542 - rbsK b3752 0.24 +5.6 3,935,551 + rbsK b3752 0.25 +2.8 3,935,551 + rbsK b3752 0.25 +3.0 3,935,560 - rbsK b3752 0.26 +5.3 3,935,577 - rbsK b3752 0.28 +5.6 3,935,650 + rbsK b3752 0.36 +2.2 3,935,656 - rbsK b3752 0.36 +5.9 3,935,661 + rbsK b3752 0.37 +2.3 3,935,666 + rbsK b3752 0.38 +4.7 3,935,669 - rbsK b3752 0.38 +5.1 3,935,669 - rbsK b3752 0.38 +6.0 3,935,669 - rbsK b3752 0.38 +4.7 3,935,711 + rbsK b3752 0.42 +2.3 3,935,742 + rbsK b3752 0.46 -0.4 3,935,798 - rbsK b3752 0.52 +4.7 3,935,798 - rbsK b3752 0.52 +5.3 3,935,808 + rbsK b3752 0.53 +1.6 3,935,942 + rbsK b3752 0.67 +1.8 3,935,946 + rbsK b3752 0.68 +0.7 3,935,946 + rbsK b3752 0.68 +0.7 3,935,955 - rbsK b3752 0.69 +5.6 3,935,969 + rbsK b3752 0.70 +1.1 3,935,969 + rbsK b3752 0.70 +0.4 3,935,977 - rbsK b3752 0.71 +5.2 3,935,977 - rbsK b3752 0.71 +5.9 3,935,983 + rbsK b3752 0.72 -0.7 3,936,007 - rbsK b3752 0.74 +4.5 3,936,007 - rbsK b3752 0.74 +5.9 3,936,007 - rbsK b3752 0.74 +4.8 3,936,009 - rbsK b3752 0.74 +5.3 3,936,028 - rbsK b3752 0.76 +5.2 3,936,028 - rbsK b3752 0.76 +5.6 3,936,048 + rbsK b3752 0.79 +0.2 3,936,055 - rbsK b3752 0.79 +6.3 3,936,074 - rbsK b3752 0.81 +5.5 3,936,074 - rbsK b3752 0.81 +5.5 3,936,082 + rbsK b3752 0.82 +1.2 3,936,082 + rbsK b3752 0.82 +1.0 3,936,197 + +1.3 3,936,338 - +5.6 3,936,338 - +5.9 3,936,395 + rbsR b3753 0.15 +6.1 3,936,407 + rbsR b3753 0.16 +5.2 3,936,415 - rbsR b3753 0.17 +7.9 3,936,444 - rbsR b3753 0.20 +5.9 3,936,447 - rbsR b3753 0.20 +5.4 3,936,456 + rbsR b3753 0.21 +5.3 3,936,456 + rbsR b3753 0.21 +5.6 3,936,463 - rbsR b3753 0.21 +5.7 3,936,470 + rbsR b3753 0.22 +6.3 3,936,470 + rbsR b3753 0.22 +5.6 3,936,473 - rbsR b3753 0.22 +4.4 3,936,473 - rbsR b3753 0.22 +5.4 3,936,496 - rbsR b3753 0.25 +5.7 3,936,496 - rbsR b3753 0.25 +4.6 3,936,545 + rbsR b3753 0.30 +5.3 3,936,659 + rbsR b3753 0.41 +5.8 3,936,667 + rbsR b3753 0.42 +6.4 3,936,667 + rbsR b3753 0.42 +5.1 3,936,667 + rbsR b3753 0.42 +4.8 3,936,684 - rbsR b3753 0.44 +4.4 3,936,684 - rbsR b3753 0.44 +4.8 3,936,686 + rbsR b3753 0.44 +5.4 3,936,723 - rbsR b3753 0.48 +5.3 3,936,726 - rbsR b3753 0.48 +4.6 3,936,763 - rbsR b3753 0.52 +4.7 3,936,769 + rbsR b3753 0.52 +5.1 3,936,794 + rbsR b3753 0.55 +5.9 3,936,842 - rbsR b3753 0.60 +5.9 3,936,868 + rbsR b3753 0.62 +5.7 3,936,868 + rbsR b3753 0.62 +5.5 3,936,868 + rbsR b3753 0.62 +6.0 3,936,875 + rbsR b3753 0.63 +6.0 3,936,876 - rbsR b3753 0.63 +6.0 3,936,876 - rbsR b3753 0.63 +5.7 3,936,878 + rbsR b3753 0.63 +5.3 3,936,878 + rbsR b3753 0.63 +5.2 3,936,943 + rbsR b3753 0.70 +5.2 3,936,953 - rbsR b3753 0.71 +5.5 3,936,953 - rbsR b3753 0.71 +6.1 3,936,989 + rbsR b3753 0.74 +5.0 3,936,989 + rbsR b3753 0.74 +5.7 3,936,990 + rbsR b3753 0.75 +4.7 3,936,992 + rbsR b3753 0.75 +4.8 3,937,039 + rbsR b3753 0.79 +5.0 3,937,055 - rbsR b3753 0.81 +6.5 3,937,101 + rbsR b3753 0.86 +4.9 3,937,124 - rbsR b3753 0.88 +5.3 3,937,124 - rbsR b3753 0.88 +5.7 3,937,130 - rbsR b3753 0.89 +5.5 3,937,137 + rbsR b3753 0.89 +6.4 3,937,174 + +6.6 3,937,174 + +6.4 3,937,177 - +5.1 3,937,184 - +4.3 3,937,186 + +4.8 3,937,186 + +5.6 3,937,201 + +4.1 3,937,205 + +5.5 3,937,244 - -0.6 3,937,244 - -0.4
Or see this region's nucleotide sequence