Strain Fitness in Escherichia coli BW25113 around b2742

Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoS and nlpD are separated by 62 nucleotidesnlpD and pcm are separated by 139 nucleotidespcm and surE overlap by 7 nucleotides b2741: rpoS - RNA polymerase sigma factor (NCBI), at 2,864,581 to 2,865,573 rpoS b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775 nlpD b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541 pcm b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296 surE Position (kb) 2865 2866 2867Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 2864.641 kb on + strandat 2864.656 kb on - strandat 2864.698 kb on + strand, within rpoSat 2864.840 kb on - strandat 2864.866 kb on + strand, within rpoSat 2864.925 kb on + strand, within rpoSat 2864.925 kb on + strand, within rpoSat 2865.021 kb on + strand, within rpoSat 2865.037 kb on + strand, within rpoSat 2865.044 kb on + strand, within rpoSat 2865.047 kb on - strand, within rpoSat 2865.085 kb on - strand, within rpoSat 2865.116 kb on - strand, within rpoSat 2865.175 kb on + strand, within rpoSat 2865.190 kb on + strand, within rpoSat 2865.195 kb on + strand, within rpoSat 2865.198 kb on - strand, within rpoSat 2865.376 kb on - strand, within rpoSat 2865.389 kb on + strand, within rpoSat 2865.415 kb on + strand, within rpoSat 2865.447 kb on - strand, within rpoSat 2865.490 kb on - strandat 2865.512 kb on - strandat 2865.598 kb on - strandat 2865.598 kb on - strandat 2865.764 kb on - strand, within nlpDat 2865.788 kb on + strand, within nlpDat 2865.788 kb on + strand, within nlpDat 2865.805 kb on - strand, within nlpDat 2865.817 kb on - strand, within nlpDat 2865.867 kb on + strand, within nlpDat 2866.116 kb on + strand, within nlpDat 2866.116 kb on + strand, within nlpDat 2866.149 kb on + strand, within nlpDat 2866.172 kb on + strand, within nlpDat 2866.210 kb on + strand, within nlpDat 2866.212 kb on + strand, within nlpDat 2866.265 kb on - strand, within nlpDat 2866.380 kb on + strand, within nlpDat 2866.380 kb on + strand, within nlpDat 2866.414 kb on - strand, within nlpDat 2866.414 kb on - strand, within nlpDat 2866.436 kb on + strand, within nlpDat 2866.441 kb on + strand, within nlpDat 2866.463 kb on - strand, within nlpDat 2866.463 kb on - strand, within nlpDat 2866.511 kb on - strand, within nlpDat 2866.511 kb on - strand, within nlpDat 2866.658 kb on + strand, within nlpDat 2866.722 kb on - strandat 2866.836 kb on - strandat 2866.998 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.359 kb on - strand, within pcmat 2867.541 kb on - strandat 2867.541 kb on - strandat 2867.616 kb on - strand, within surEat 2867.623 kb on + strand, within surEat 2867.631 kb on + strand, within surEat 2867.672 kb on - strand, within surEat 2867.701 kb on + strand, within surEat 2867.709 kb on - strand, within surEat 2867.747 kb on + strand, within surEat 2867.747 kb on + strand, within surE

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
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2,864,641 + +2.9
2,864,656 - +6.2
2,864,698 + rpoS b2741 0.12 +5.3
2,864,840 - +2.9
2,864,866 + rpoS b2741 0.29 +4.8
2,864,925 + rpoS b2741 0.35 +3.7
2,864,925 + rpoS b2741 0.35 +3.6
2,865,021 + rpoS b2741 0.44 +5.0
2,865,037 + rpoS b2741 0.46 +4.2
2,865,044 + rpoS b2741 0.47 +4.3
2,865,047 - rpoS b2741 0.47 +5.2
2,865,085 - rpoS b2741 0.51 +4.5
2,865,116 - rpoS b2741 0.54 +4.6
2,865,175 + rpoS b2741 0.60 +3.2
2,865,190 + rpoS b2741 0.61 +4.0
2,865,195 + rpoS b2741 0.62 +3.9
2,865,198 - rpoS b2741 0.62 +4.8
2,865,376 - rpoS b2741 0.80 +4.9
2,865,389 + rpoS b2741 0.81 +5.2
2,865,415 + rpoS b2741 0.84 +5.8
2,865,447 - rpoS b2741 0.87 +5.2
2,865,490 - +3.1
2,865,512 - +5.0
2,865,598 - +0.7
2,865,598 - -0.1
2,865,764 - nlpD b2742 0.11 +1.6
2,865,788 + nlpD b2742 0.13 +4.5
2,865,788 + nlpD b2742 0.13 +4.8
2,865,805 - nlpD b2742 0.15 -0.3
2,865,817 - nlpD b2742 0.16 +0.1
2,865,867 + nlpD b2742 0.20 +2.6
2,866,116 + nlpD b2742 0.42 +3.8
2,866,116 + nlpD b2742 0.42 +3.5
2,866,149 + nlpD b2742 0.45 +2.8
2,866,172 + nlpD b2742 0.47 +0.2
2,866,210 + nlpD b2742 0.50 +0.3
2,866,212 + nlpD b2742 0.51 -0.0
2,866,265 - nlpD b2742 0.55 +0.4
2,866,380 + nlpD b2742 0.65 +1.0
2,866,380 + nlpD b2742 0.65 +1.6
2,866,414 - nlpD b2742 0.68 -0.1
2,866,414 - nlpD b2742 0.68 -0.0
2,866,436 + nlpD b2742 0.70 -0.8
2,866,441 + nlpD b2742 0.71 -1.2
2,866,463 - nlpD b2742 0.73 -0.2
2,866,463 - nlpD b2742 0.73 -0.0
2,866,511 - nlpD b2742 0.77 -0.1
2,866,511 - nlpD b2742 0.77 -0.4
2,866,658 + nlpD b2742 0.90 -0.4
2,866,722 - +0.3
2,866,836 - +1.2
2,866,998 + pcm b2743 0.13 +1.5
2,867,354 + pcm b2743 0.70 -0.5
2,867,354 + pcm b2743 0.70 +1.6
2,867,354 + pcm b2743 0.70 +0.3
2,867,354 + pcm b2743 0.70 +0.7
2,867,354 + pcm b2743 0.70 +0.8
2,867,359 - pcm b2743 0.71 +0.6
2,867,541 - +0.7
2,867,541 - +0.8
2,867,616 - surE b2744 0.11 +1.4
2,867,623 + surE b2744 0.12 -0.5
2,867,631 + surE b2744 0.13 +0.3
2,867,672 - surE b2744 0.18 -0.6
2,867,701 + surE b2744 0.22 -0.8
2,867,709 - surE b2744 0.23 -0.4
2,867,747 + surE b2744 0.28 +0.9
2,867,747 + surE b2744 0.28 +0.6

Or see this region's nucleotide sequence