Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciT and yciZ are separated by 89 nucleotides yciZ and gmr are separated by 147 nucleotides gmr and ymiB are separated by 53 nucleotides ymiB and rnb are separated by 77 nucleotides
b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370
yciT
b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633
yciZ
b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766
gmr
b4672: ymiB - expressed protein (RefSeq), at 1,344,820 to 1,344,924
ymiB
b1286: rnb - exoribonuclease II (NCBI), at 1,345,002 to 1,346,936
rnb
Position (kb)
1342
1343
1344
1345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1341.823 kb on - strand, within yciT at 1342.104 kb on + strand, within yciT at 1342.104 kb on + strand, within yciT at 1342.216 kb on - strand, within yciT at 1342.306 kb on - strand at 1342.313 kb on + strand at 1342.313 kb on + strand at 1342.329 kb on + strand at 1342.478 kb on + strand, within yciZ at 1342.478 kb on + strand, within yciZ at 1342.708 kb on - strand at 1342.809 kb on - strand at 1343.191 kb on - strand, within gmr at 1343.224 kb on + strand, within gmr at 1343.224 kb on + strand, within gmr at 1343.235 kb on + strand, within gmr at 1343.621 kb on + strand, within gmr at 1343.621 kb on + strand, within gmr at 1343.700 kb on - strand, within gmr at 1343.705 kb on + strand, within gmr at 1343.740 kb on + strand, within gmr at 1343.840 kb on + strand, within gmr at 1343.849 kb on + strand, within gmr at 1343.988 kb on + strand, within gmr at 1343.988 kb on + strand, within gmr at 1344.018 kb on + strand, within gmr at 1344.084 kb on - strand, within gmr at 1344.111 kb on + strand, within gmr at 1344.215 kb on + strand, within gmr at 1344.256 kb on - strand, within gmr at 1344.290 kb on - strand, within gmr at 1344.481 kb on + strand, within gmr at 1345.121 kb on + strand at 1345.306 kb on - strand, within rnb at 1345.321 kb on - strand, within rnb at 1345.321 kb on - strand, within rnb at 1345.492 kb on - strand, within rnb at 1345.550 kb on - strand, within rnb at 1345.727 kb on + strand, within rnb at 1345.736 kb on - strand, within rnb
Per-strain Table
Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) remove 1,341,823 - yciT b1284 0.27 +1.6 1,342,104 + yciT b1284 0.64 +0.8 1,342,104 + yciT b1284 0.64 +0.9 1,342,216 - yciT b1284 0.79 +1.8 1,342,306 - +1.9 1,342,313 + +1.8 1,342,313 + +0.4 1,342,329 + +0.8 1,342,478 + yciZ b4596 0.10 +0.1 1,342,478 + yciZ b4596 0.10 +1.3 1,342,708 - -0.4 1,342,809 - -1.0 1,343,191 - gmr b1285 0.21 -0.8 1,343,224 + gmr b1285 0.22 -1.0 1,343,224 + gmr b1285 0.22 -1.1 1,343,235 + gmr b1285 0.23 -0.6 1,343,621 + gmr b1285 0.42 -1.8 1,343,621 + gmr b1285 0.42 -1.1 1,343,700 - gmr b1285 0.46 -0.9 1,343,705 + gmr b1285 0.47 -1.6 1,343,740 + gmr b1285 0.48 -2.9 1,343,840 + gmr b1285 0.53 -1.7 1,343,849 + gmr b1285 0.54 -2.5 1,343,988 + gmr b1285 0.61 +0.3 1,343,988 + gmr b1285 0.61 +0.2 1,344,018 + gmr b1285 0.62 -0.3 1,344,084 - gmr b1285 0.66 -1.9 1,344,111 + gmr b1285 0.67 -0.7 1,344,215 + gmr b1285 0.72 -1.1 1,344,256 - gmr b1285 0.74 -2.7 1,344,290 - gmr b1285 0.76 -1.4 1,344,481 + gmr b1285 0.86 -1.2 1,345,121 + +2.3 1,345,306 - rnb b1286 0.16 +2.0 1,345,321 - rnb b1286 0.16 +1.9 1,345,321 - rnb b1286 0.16 +0.5 1,345,492 - rnb b1286 0.25 +3.6 1,345,550 - rnb b1286 0.28 +0.2 1,345,727 + rnb b1286 0.37 +1.6 1,345,736 - rnb b1286 0.38 +1.8
Or see this region's nucleotide sequence