Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt udk and yegE are separated by 317 nucleotides yegE and alkA are separated by 108 nucleotides
b2066: udk - uridine/cytidine kinase (VIMSS), at 2,140,331 to 2,140,972
udk
b2067: yegE - predicted diguanylate cyclase, GGDEF domain signalling protein (NCBI), at 2,141,290 to 2,144,607
yegE
b2068: alkA - 3-methyl-adenine DNA glycosylase II (NCBI), at 2,144,716 to 2,145,564
alkA
Position (kb)
2141
2142
2143
2144
2145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2140.303 kb on + strand at 2140.303 kb on + strand at 2140.303 kb on + strand at 2140.311 kb on - strand at 2140.363 kb on + strand at 2140.494 kb on - strand at 2140.494 kb on - strand, within udk at 2140.505 kb on + strand, within udk at 2140.640 kb on - strand, within udk at 2140.769 kb on - strand, within udk at 2140.772 kb on + strand, within udk at 2140.772 kb on + strand, within udk at 2140.819 kb on - strand, within udk at 2140.819 kb on - strand, within udk at 2140.848 kb on - strand, within udk at 2140.861 kb on - strand, within udk at 2140.906 kb on - strand, within udk at 2140.940 kb on + strand at 2140.952 kb on - strand at 2140.968 kb on + strand at 2140.976 kb on - strand at 2140.995 kb on - strand at 2141.000 kb on + strand at 2141.007 kb on - strand at 2141.245 kb on - strand at 2141.265 kb on + strand at 2141.351 kb on + strand at 2141.403 kb on + strand at 2141.403 kb on + strand at 2141.430 kb on - strand at 2141.435 kb on - strand at 2141.435 kb on - strand at 2141.595 kb on + strand at 2141.608 kb on - strand at 2141.608 kb on - strand at 2141.631 kb on + strand, within yegE at 2141.631 kb on + strand, within yegE at 2141.631 kb on + strand, within yegE at 2141.631 kb on + strand, within yegE at 2141.639 kb on - strand, within yegE at 2141.639 kb on - strand, within yegE at 2141.639 kb on - strand, within yegE at 2142.076 kb on + strand, within yegE at 2142.076 kb on + strand, within yegE at 2142.132 kb on + strand, within yegE at 2142.140 kb on - strand, within yegE at 2142.140 kb on - strand, within yegE at 2142.259 kb on - strand, within yegE at 2142.302 kb on + strand, within yegE at 2142.519 kb on + strand, within yegE at 2142.555 kb on + strand, within yegE at 2142.555 kb on + strand, within yegE at 2142.560 kb on + strand, within yegE at 2142.560 kb on + strand, within yegE at 2142.583 kb on + strand, within yegE at 2142.583 kb on + strand, within yegE at 2142.588 kb on + strand, within yegE at 2142.722 kb on - strand, within yegE at 2142.722 kb on - strand, within yegE at 2142.812 kb on + strand, within yegE at 2142.924 kb on + strand, within yegE at 2142.924 kb on + strand, within yegE at 2143.046 kb on + strand, within yegE at 2143.104 kb on - strand, within yegE at 2143.183 kb on - strand, within yegE at 2143.246 kb on + strand, within yegE at 2143.281 kb on - strand, within yegE at 2143.282 kb on + strand, within yegE at 2143.297 kb on - strand, within yegE at 2143.339 kb on - strand, within yegE at 2143.476 kb on - strand, within yegE at 2143.478 kb on - strand, within yegE at 2143.478 kb on - strand, within yegE at 2143.531 kb on - strand, within yegE at 2143.858 kb on - strand, within yegE at 2143.862 kb on + strand, within yegE at 2143.862 kb on + strand, within yegE at 2143.864 kb on + strand, within yegE at 2143.894 kb on + strand, within yegE at 2143.894 kb on + strand, within yegE at 2144.000 kb on - strand, within yegE at 2144.201 kb on + strand, within yegE at 2144.299 kb on + strand at 2144.299 kb on + strand at 2144.383 kb on - strand at 2144.501 kb on + strand at 2144.614 kb on + strand at 2144.721 kb on - strand at 2144.721 kb on - strand at 2144.753 kb on + strand at 2144.877 kb on + strand, within alkA at 2144.963 kb on - strand, within alkA at 2145.185 kb on + strand, within alkA at 2145.267 kb on + strand, within alkA at 2145.336 kb on + strand, within alkA at 2145.336 kb on + strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.452 kb on - strand, within alkA at 2145.480 kb on + strand at 2145.540 kb on - strand at 2145.557 kb on + strand at 2145.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) remove 2,140,303 + +0.4 2,140,303 + -1.9 2,140,303 + -0.0 2,140,311 - -0.1 2,140,363 + +0.1 2,140,494 - +0.9 2,140,494 - udk b2066 0.25 +0.6 2,140,505 + udk b2066 0.27 +0.4 2,140,640 - udk b2066 0.48 -0.0 2,140,769 - udk b2066 0.68 +0.2 2,140,772 + udk b2066 0.69 -0.0 2,140,772 + udk b2066 0.69 -1.6 2,140,819 - udk b2066 0.76 -3.0 2,140,819 - udk b2066 0.76 +0.9 2,140,848 - udk b2066 0.81 +0.1 2,140,861 - udk b2066 0.83 -1.1 2,140,906 - udk b2066 0.90 +0.2 2,140,940 + -1.4 2,140,952 - +0.5 2,140,968 + +1.3 2,140,976 - +0.6 2,140,995 - -0.8 2,141,000 + -0.3 2,141,007 - -0.0 2,141,245 - +1.8 2,141,265 + +0.8 2,141,351 + +3.0 2,141,403 + +0.9 2,141,403 + +1.2 2,141,430 - +1.8 2,141,435 - +0.8 2,141,435 - +1.5 2,141,595 + +1.6 2,141,608 - +1.0 2,141,608 - +1.2 2,141,631 + yegE b2067 0.10 +1.5 2,141,631 + yegE b2067 0.10 +0.3 2,141,631 + yegE b2067 0.10 +1.0 2,141,631 + yegE b2067 0.10 +1.1 2,141,639 - yegE b2067 0.11 +2.1 2,141,639 - yegE b2067 0.11 +1.8 2,141,639 - yegE b2067 0.11 +1.7 2,142,076 + yegE b2067 0.24 +1.1 2,142,076 + yegE b2067 0.24 +0.7 2,142,132 + yegE b2067 0.25 +1.8 2,142,140 - yegE b2067 0.26 +1.8 2,142,140 - yegE b2067 0.26 +0.6 2,142,259 - yegE b2067 0.29 +0.8 2,142,302 + yegE b2067 0.31 +1.9 2,142,519 + yegE b2067 0.37 +1.2 2,142,555 + yegE b2067 0.38 +1.1 2,142,555 + yegE b2067 0.38 +1.8 2,142,560 + yegE b2067 0.38 +1.2 2,142,560 + yegE b2067 0.38 +1.4 2,142,583 + yegE b2067 0.39 +0.0 2,142,583 + yegE b2067 0.39 +2.0 2,142,588 + yegE b2067 0.39 +0.4 2,142,722 - yegE b2067 0.43 +2.3 2,142,722 - yegE b2067 0.43 +0.1 2,142,812 + yegE b2067 0.46 +1.7 2,142,924 + yegE b2067 0.49 +0.8 2,142,924 + yegE b2067 0.49 -0.1 2,143,046 + yegE b2067 0.53 +1.8 2,143,104 - yegE b2067 0.55 +1.0 2,143,183 - yegE b2067 0.57 +1.3 2,143,246 + yegE b2067 0.59 +0.1 2,143,281 - yegE b2067 0.60 +4.2 2,143,282 + yegE b2067 0.60 +1.3 2,143,297 - yegE b2067 0.60 +1.6 2,143,339 - yegE b2067 0.62 +1.6 2,143,476 - yegE b2067 0.66 +0.1 2,143,478 - yegE b2067 0.66 +0.4 2,143,478 - yegE b2067 0.66 +0.7 2,143,531 - yegE b2067 0.68 +0.8 2,143,858 - yegE b2067 0.77 +2.2 2,143,862 + yegE b2067 0.78 +1.0 2,143,862 + yegE b2067 0.78 +0.9 2,143,864 + yegE b2067 0.78 +0.3 2,143,894 + yegE b2067 0.78 +0.9 2,143,894 + yegE b2067 0.78 +1.8 2,144,000 - yegE b2067 0.82 +1.6 2,144,201 + yegE b2067 0.88 +1.1 2,144,299 + +1.0 2,144,299 + +1.6 2,144,383 - +2.0 2,144,501 + +1.4 2,144,614 + -1.0 2,144,721 - -0.1 2,144,721 - +0.5 2,144,753 + -0.7 2,144,877 + alkA b2068 0.19 +0.4 2,144,963 - alkA b2068 0.29 -0.6 2,145,185 + alkA b2068 0.55 -0.6 2,145,267 + alkA b2068 0.65 +0.4 2,145,336 + alkA b2068 0.73 +0.4 2,145,336 + alkA b2068 0.73 +1.8 2,145,398 - alkA b2068 0.80 +0.8 2,145,398 - alkA b2068 0.80 +0.9 2,145,398 - alkA b2068 0.80 -0.2 2,145,398 - alkA b2068 0.80 -1.9 2,145,452 - alkA b2068 0.87 -1.1 2,145,480 + -0.2 2,145,540 - +0.7 2,145,557 + -1.1 2,145,565 - +0.1
Or see this region's nucleotide sequence