Strain Fitness in Escherichia coli BW25113 around b1128

Experiment: LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepT and ycfD are separated by 48 nucleotidesycfD and phoQ are separated by 75 nucleotides b1127: pepT - peptidase T (NCBI), at 1,185,067 to 1,186,293 pepT b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463 ycfD b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999 phoQ Position (kb) 1186 1187 1188Strain fitness (log2 ratio) -2 -1 0 1at 1185.409 kb on + strand, within pepTat 1185.639 kb on + strand, within pepTat 1185.647 kb on + strand, within pepTat 1185.720 kb on + strand, within pepTat 1185.746 kb on - strand, within pepTat 1185.779 kb on - strand, within pepTat 1185.886 kb on + strand, within pepTat 1185.986 kb on - strand, within pepTat 1186.009 kb on + strand, within pepTat 1186.182 kb on - strandat 1186.468 kb on - strand, within ycfDat 1186.507 kb on - strand, within ycfDat 1186.533 kb on - strand, within ycfDat 1186.778 kb on + strand, within ycfDat 1186.778 kb on + strand, within ycfDat 1186.805 kb on - strand, within ycfDat 1186.876 kb on - strand, within ycfDat 1186.942 kb on - strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1187.202 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfDat 1187.349 kb on + strand, within ycfDat 1187.349 kb on + strand, within ycfDat 1187.446 kb on + strandat 1187.521 kb on + strandat 1187.672 kb on - strandat 1187.672 kb on - strandat 1187.674 kb on - strandat 1187.674 kb on - strandat 1187.685 kb on + strand, within phoQat 1187.775 kb on - strand, within phoQat 1187.776 kb on - strand, within phoQat 1187.776 kb on - strand, within phoQat 1187.779 kb on + strand, within phoQat 1187.844 kb on - strand, within phoQat 1187.955 kb on + strand, within phoQat 1187.973 kb on - strand, within phoQat 1188.100 kb on - strand, within phoQat 1188.191 kb on + strand, within phoQat 1188.246 kb on + strand, within phoQat 1188.320 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.461 kb on + strand, within phoQ

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
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1,185,409 + pepT b1127 0.28 +0.4
1,185,639 + pepT b1127 0.47 +0.2
1,185,647 + pepT b1127 0.47 +0.9
1,185,720 + pepT b1127 0.53 +0.5
1,185,746 - pepT b1127 0.55 +0.2
1,185,779 - pepT b1127 0.58 +1.1
1,185,886 + pepT b1127 0.67 +0.5
1,185,986 - pepT b1127 0.75 +1.6
1,186,009 + pepT b1127 0.77 -0.2
1,186,182 - +0.4
1,186,468 - ycfD b1128 0.11 +1.3
1,186,507 - ycfD b1128 0.15 +0.3
1,186,533 - ycfD b1128 0.17 +1.2
1,186,778 + ycfD b1128 0.39 +1.1
1,186,778 + ycfD b1128 0.39 +0.3
1,186,805 - ycfD b1128 0.41 +0.9
1,186,876 - ycfD b1128 0.48 +1.6
1,186,942 - ycfD b1128 0.53 +1.0
1,186,950 + ycfD b1128 0.54 +0.9
1,186,950 + ycfD b1128 0.54 +0.9
1,187,202 + ycfD b1128 0.77 +1.4
1,187,292 + ycfD b1128 0.85 +0.4
1,187,292 + ycfD b1128 0.85 +0.3
1,187,349 + ycfD b1128 0.90 -0.7
1,187,349 + ycfD b1128 0.90 +1.1
1,187,446 + +0.6
1,187,521 + -0.2
1,187,672 - -1.4
1,187,672 - -0.1
1,187,674 - -0.9
1,187,674 - -1.4
1,187,685 + phoQ b1129 0.10 +1.4
1,187,775 - phoQ b1129 0.16 -1.6
1,187,776 - phoQ b1129 0.16 -1.6
1,187,776 - phoQ b1129 0.16 -0.1
1,187,779 + phoQ b1129 0.16 -0.9
1,187,844 - phoQ b1129 0.21 -0.9
1,187,955 + phoQ b1129 0.28 -1.1
1,187,973 - phoQ b1129 0.30 -1.7
1,188,100 - phoQ b1129 0.38 +1.4
1,188,191 + phoQ b1129 0.45 +1.4
1,188,246 + phoQ b1129 0.48 +1.4
1,188,320 + phoQ b1129 0.53 -2.1
1,188,342 + phoQ b1129 0.55 +0.4
1,188,342 + phoQ b1129 0.55 -1.4
1,188,435 - phoQ b1129 0.61 +0.4
1,188,435 - phoQ b1129 0.61 -1.9
1,188,461 + phoQ b1129 0.63 -0.6

Or see this region's nucleotide sequence