Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpK and glpF are separated by 22 nucleotides glpF and yiiU are separated by 424 nucleotides yiiU and rraA are separated by 84 nucleotides
b3926: glpK - glycerol kinase (NCBI), at 4,113,737 to 4,115,245
glpK
b3927: glpF - glycerol facilitator (NCBI), at 4,115,268 to 4,116,113
glpF
b3928: yiiU - hypothetical protein (NCBI), at 4,116,538 to 4,116,783
yiiU
b3929: rraA - ribonuclease activity regulator protein RraA (NCBI), at 4,116,868 to 4,117,353
rraA
Position (kb)
4115
4116
4117 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4114.318 kb on + strand, within glpK at 4114.342 kb on - strand, within glpK at 4114.429 kb on + strand, within glpK at 4114.429 kb on + strand, within glpK at 4114.437 kb on - strand, within glpK at 4114.437 kb on - strand, within glpK at 4114.437 kb on - strand, within glpK at 4114.437 kb on - strand, within glpK at 4114.453 kb on - strand, within glpK at 4114.474 kb on - strand, within glpK at 4114.510 kb on - strand, within glpK at 4114.539 kb on - strand, within glpK at 4114.557 kb on + strand, within glpK at 4114.557 kb on + strand, within glpK at 4114.569 kb on - strand, within glpK at 4114.656 kb on + strand, within glpK at 4114.657 kb on - strand, within glpK at 4114.664 kb on + strand, within glpK at 4114.664 kb on + strand, within glpK at 4114.684 kb on - strand, within glpK at 4114.684 kb on - strand, within glpK at 4114.703 kb on + strand, within glpK at 4114.703 kb on + strand, within glpK at 4114.710 kb on + strand, within glpK at 4114.710 kb on + strand, within glpK at 4114.723 kb on - strand, within glpK at 4114.723 kb on - strand, within glpK at 4114.738 kb on + strand, within glpK at 4114.743 kb on + strand, within glpK at 4114.750 kb on - strand, within glpK at 4114.770 kb on - strand, within glpK at 4114.775 kb on + strand, within glpK at 4114.777 kb on + strand, within glpK at 4114.784 kb on + strand, within glpK at 4114.794 kb on - strand, within glpK at 4114.814 kb on + strand, within glpK at 4114.817 kb on + strand, within glpK at 4114.833 kb on + strand, within glpK at 4114.870 kb on + strand, within glpK at 4114.887 kb on - strand at 4114.939 kb on - strand, within glpK at 4114.939 kb on - strand, within glpK at 4114.951 kb on + strand, within glpK at 4114.951 kb on + strand, within glpK at 4114.952 kb on - strand, within glpK at 4114.989 kb on - strand, within glpK at 4114.989 kb on - strand, within glpK at 4115.009 kb on + strand, within glpK at 4115.009 kb on + strand, within glpK at 4115.010 kb on - strand, within glpK at 4115.010 kb on - strand, within glpK at 4115.044 kb on - strand, within glpK at 4115.055 kb on + strand, within glpK at 4115.063 kb on - strand, within glpK at 4115.063 kb on - strand, within glpK at 4115.065 kb on + strand, within glpK at 4115.065 kb on + strand, within glpK at 4115.072 kb on + strand, within glpK at 4115.073 kb on - strand, within glpK at 4115.081 kb on + strand, within glpK at 4115.087 kb on + strand, within glpK at 4115.099 kb on + strand at 4115.100 kb on - strand at 4115.102 kb on + strand at 4115.102 kb on + strand at 4115.112 kb on - strand at 4115.112 kb on - strand at 4115.112 kb on - strand at 4115.113 kb on + strand at 4115.157 kb on - strand at 4115.164 kb on - strand at 4115.164 kb on - strand at 4115.167 kb on - strand at 4115.171 kb on + strand at 4115.180 kb on + strand at 4115.182 kb on - strand at 4115.184 kb on - strand at 4115.184 kb on - strand at 4115.188 kb on - strand at 4115.188 kb on - strand at 4115.254 kb on - strand at 4115.254 kb on - strand at 4115.262 kb on + strand at 4115.265 kb on + strand at 4115.285 kb on + strand at 4115.296 kb on + strand at 4115.325 kb on + strand at 4115.363 kb on + strand, within glpF at 4115.363 kb on + strand, within glpF at 4115.371 kb on + strand, within glpF at 4115.381 kb on - strand, within glpF at 4115.388 kb on + strand, within glpF at 4115.415 kb on + strand, within glpF at 4115.430 kb on - strand, within glpF at 4115.431 kb on - strand, within glpF at 4115.449 kb on + strand, within glpF at 4115.462 kb on + strand, within glpF at 4115.544 kb on + strand, within glpF at 4115.544 kb on + strand, within glpF at 4115.549 kb on - strand, within glpF at 4115.552 kb on + strand, within glpF at 4115.552 kb on + strand, within glpF at 4115.564 kb on - strand, within glpF at 4115.566 kb on - strand, within glpF at 4115.577 kb on + strand, within glpF at 4115.585 kb on - strand, within glpF at 4115.585 kb on - strand, within glpF at 4115.589 kb on + strand, within glpF at 4115.589 kb on + strand, within glpF at 4115.602 kb on - strand, within glpF at 4115.602 kb on - strand, within glpF at 4115.602 kb on - strand, within glpF at 4115.602 kb on - strand, within glpF at 4115.612 kb on - strand, within glpF at 4115.612 kb on - strand, within glpF at 4115.632 kb on - strand, within glpF at 4115.632 kb on - strand, within glpF at 4115.634 kb on + strand, within glpF at 4115.642 kb on - strand, within glpF at 4115.647 kb on - strand, within glpF at 4115.657 kb on + strand, within glpF at 4115.692 kb on + strand, within glpF at 4115.723 kb on + strand, within glpF at 4115.742 kb on + strand, within glpF at 4115.742 kb on + strand, within glpF at 4115.761 kb on - strand, within glpF at 4115.793 kb on - strand, within glpF at 4115.793 kb on - strand, within glpF at 4115.865 kb on - strand, within glpF at 4115.865 kb on - strand, within glpF at 4115.886 kb on + strand, within glpF at 4115.889 kb on + strand, within glpF at 4115.893 kb on - strand, within glpF at 4115.953 kb on + strand, within glpF at 4115.956 kb on + strand, within glpF at 4115.956 kb on + strand, within glpF at 4115.960 kb on + strand, within glpF at 4115.960 kb on + strand, within glpF at 4115.964 kb on - strand, within glpF at 4115.966 kb on + strand, within glpF at 4115.968 kb on - strand, within glpF at 4116.014 kb on + strand, within glpF at 4116.023 kb on + strand, within glpF at 4116.023 kb on + strand, within glpF at 4116.027 kb on + strand, within glpF at 4116.036 kb on - strand at 4116.056 kb on + strand at 4116.081 kb on + strand at 4116.101 kb on + strand at 4116.101 kb on + strand at 4116.103 kb on + strand at 4116.138 kb on - strand at 4116.148 kb on + strand at 4116.171 kb on + strand at 4116.174 kb on + strand at 4116.175 kb on + strand at 4116.176 kb on + strand at 4116.193 kb on - strand at 4116.236 kb on + strand at 4116.236 kb on + strand at 4116.236 kb on - strand at 4116.236 kb on - strand at 4116.241 kb on - strand at 4116.241 kb on - strand at 4116.289 kb on + strand at 4116.289 kb on + strand at 4116.292 kb on + strand at 4116.293 kb on - strand at 4116.355 kb on - strand at 4116.360 kb on - strand at 4116.381 kb on + strand at 4116.398 kb on + strand at 4116.415 kb on + strand at 4116.415 kb on + strand at 4116.415 kb on + strand at 4116.418 kb on - strand at 4116.420 kb on + strand at 4116.459 kb on - strand at 4116.528 kb on - strand at 4116.533 kb on - strand at 4116.534 kb on + strand at 4116.535 kb on + strand at 4116.542 kb on - strand at 4116.543 kb on - strand at 4116.543 kb on - strand at 4116.548 kb on + strand at 4116.548 kb on + strand at 4116.548 kb on + strand at 4116.564 kb on - strand, within yiiU at 4116.580 kb on + strand, within yiiU at 4116.592 kb on + strand, within yiiU at 4116.597 kb on + strand, within yiiU at 4116.599 kb on + strand, within yiiU at 4116.599 kb on + strand, within yiiU at 4116.603 kb on - strand, within yiiU at 4116.607 kb on + strand, within yiiU at 4116.647 kb on + strand, within yiiU at 4116.647 kb on + strand, within yiiU at 4116.654 kb on + strand, within yiiU at 4116.683 kb on + strand, within yiiU at 4116.686 kb on - strand, within yiiU at 4116.686 kb on - strand, within yiiU at 4116.691 kb on + strand, within yiiU at 4116.691 kb on + strand, within yiiU at 4116.748 kb on + strand, within yiiU at 4116.767 kb on + strand at 4116.767 kb on + strand at 4116.767 kb on + strand at 4116.775 kb on - strand at 4116.782 kb on + strand at 4116.782 kb on + strand at 4116.785 kb on - strand at 4116.789 kb on - strand at 4116.789 kb on - strand at 4116.816 kb on + strand at 4116.816 kb on + strand at 4116.816 kb on + strand at 4116.824 kb on - strand at 4116.824 kb on - strand at 4116.824 kb on - strand at 4116.824 kb on - strand at 4116.824 kb on - strand at 4116.858 kb on + strand at 4116.883 kb on - strand at 4116.883 kb on - strand at 4116.930 kb on + strand, within rraA at 4116.930 kb on + strand, within rraA at 4116.932 kb on - strand, within rraA at 4116.932 kb on - strand, within rraA at 4116.940 kb on + strand, within rraA at 4116.960 kb on - strand, within rraA at 4117.029 kb on - strand, within rraA at 4117.029 kb on - strand, within rraA at 4117.039 kb on - strand, within rraA at 4117.050 kb on - strand, within rraA at 4117.083 kb on - strand, within rraA at 4117.083 kb on - strand, within rraA
Per-strain Table
Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) remove 4,114,318 + glpK b3926 0.39 -2.8 4,114,342 - glpK b3926 0.40 -3.2 4,114,429 + glpK b3926 0.46 -3.8 4,114,429 + glpK b3926 0.46 -3.9 4,114,437 - glpK b3926 0.46 -3.4 4,114,437 - glpK b3926 0.46 -1.5 4,114,437 - glpK b3926 0.46 -4.0 4,114,437 - glpK b3926 0.46 -1.7 4,114,453 - glpK b3926 0.47 -1.6 4,114,474 - glpK b3926 0.49 -3.0 4,114,510 - glpK b3926 0.51 -4.0 4,114,539 - glpK b3926 0.53 -1.3 4,114,557 + glpK b3926 0.54 -3.1 4,114,557 + glpK b3926 0.54 -3.2 4,114,569 - glpK b3926 0.55 -4.0 4,114,656 + glpK b3926 0.61 -1.8 4,114,657 - glpK b3926 0.61 -1.3 4,114,664 + glpK b3926 0.61 -1.2 4,114,664 + glpK b3926 0.61 -2.7 4,114,684 - glpK b3926 0.63 -2.9 4,114,684 - glpK b3926 0.63 -0.2 4,114,703 + glpK b3926 0.64 -2.6 4,114,703 + glpK b3926 0.64 -3.0 4,114,710 + glpK b3926 0.64 -0.8 4,114,710 + glpK b3926 0.64 -0.8 4,114,723 - glpK b3926 0.65 -4.6 4,114,723 - glpK b3926 0.65 -2.0 4,114,738 + glpK b3926 0.66 -4.0 4,114,743 + glpK b3926 0.67 -2.4 4,114,750 - glpK b3926 0.67 -3.8 4,114,770 - glpK b3926 0.68 -1.8 4,114,775 + glpK b3926 0.69 -1.9 4,114,777 + glpK b3926 0.69 -4.4 4,114,784 + glpK b3926 0.69 -3.8 4,114,794 - glpK b3926 0.70 -4.5 4,114,814 + glpK b3926 0.71 -3.2 4,114,817 + glpK b3926 0.72 -3.9 4,114,833 + glpK b3926 0.73 -3.5 4,114,870 + glpK b3926 0.75 -1.1 4,114,887 - -0.7 4,114,939 - glpK b3926 0.80 -2.8 4,114,939 - glpK b3926 0.80 -4.2 4,114,951 + glpK b3926 0.80 -1.4 4,114,951 + glpK b3926 0.80 -4.4 4,114,952 - glpK b3926 0.81 -3.7 4,114,989 - glpK b3926 0.83 -2.8 4,114,989 - glpK b3926 0.83 -2.5 4,115,009 + glpK b3926 0.84 -2.9 4,115,009 + glpK b3926 0.84 -2.0 4,115,010 - glpK b3926 0.84 -1.6 4,115,010 - glpK b3926 0.84 -1.6 4,115,044 - glpK b3926 0.87 -2.9 4,115,055 + glpK b3926 0.87 -3.4 4,115,063 - glpK b3926 0.88 -3.9 4,115,063 - glpK b3926 0.88 -4.0 4,115,065 + glpK b3926 0.88 -1.8 4,115,065 + glpK b3926 0.88 -2.4 4,115,072 + glpK b3926 0.88 -3.3 4,115,073 - glpK b3926 0.89 -3.6 4,115,081 + glpK b3926 0.89 -4.4 4,115,087 + glpK b3926 0.89 -1.0 4,115,099 + -3.2 4,115,100 - -3.1 4,115,102 + -3.3 4,115,102 + -1.5 4,115,112 - -3.2 4,115,112 - -2.0 4,115,112 - -3.3 4,115,113 + -2.8 4,115,157 - -1.8 4,115,164 - -1.8 4,115,164 - -5.4 4,115,167 - -1.3 4,115,171 + -4.2 4,115,180 + -3.3 4,115,182 - -2.8 4,115,184 - -3.9 4,115,184 - -1.1 4,115,188 - -2.4 4,115,188 - -3.4 4,115,254 - -1.8 4,115,254 - -3.0 4,115,262 + -2.5 4,115,265 + -0.8 4,115,285 + -2.2 4,115,296 + -1.2 4,115,325 + -4.5 4,115,363 + glpF b3927 0.11 -2.0 4,115,363 + glpF b3927 0.11 -2.3 4,115,371 + glpF b3927 0.12 -3.0 4,115,381 - glpF b3927 0.13 -0.9 4,115,388 + glpF b3927 0.14 -2.6 4,115,415 + glpF b3927 0.17 -3.9 4,115,430 - glpF b3927 0.19 -0.5 4,115,431 - glpF b3927 0.19 -1.2 4,115,449 + glpF b3927 0.21 -3.9 4,115,462 + glpF b3927 0.23 -4.2 4,115,544 + glpF b3927 0.33 -2.9 4,115,544 + glpF b3927 0.33 -2.7 4,115,549 - glpF b3927 0.33 -1.3 4,115,552 + glpF b3927 0.34 -0.9 4,115,552 + glpF b3927 0.34 -1.6 4,115,564 - glpF b3927 0.35 +0.1 4,115,566 - glpF b3927 0.35 -1.0 4,115,577 + glpF b3927 0.37 -1.4 4,115,585 - glpF b3927 0.37 +0.8 4,115,585 - glpF b3927 0.37 -0.2 4,115,589 + glpF b3927 0.38 -2.8 4,115,589 + glpF b3927 0.38 -1.9 4,115,602 - glpF b3927 0.39 -0.9 4,115,602 - glpF b3927 0.39 -0.8 4,115,602 - glpF b3927 0.39 -2.1 4,115,602 - glpF b3927 0.39 -0.9 4,115,612 - glpF b3927 0.41 -1.6 4,115,612 - glpF b3927 0.41 -0.9 4,115,632 - glpF b3927 0.43 -0.4 4,115,632 - glpF b3927 0.43 -1.2 4,115,634 + glpF b3927 0.43 -2.7 4,115,642 - glpF b3927 0.44 -1.3 4,115,647 - glpF b3927 0.45 -1.9 4,115,657 + glpF b3927 0.46 -1.3 4,115,692 + glpF b3927 0.50 -2.0 4,115,723 + glpF b3927 0.54 -2.6 4,115,742 + glpF b3927 0.56 -3.1 4,115,742 + glpF b3927 0.56 -2.7 4,115,761 - glpF b3927 0.58 -0.9 4,115,793 - glpF b3927 0.62 -1.8 4,115,793 - glpF b3927 0.62 -3.6 4,115,865 - glpF b3927 0.71 -0.9 4,115,865 - glpF b3927 0.71 -1.5 4,115,886 + glpF b3927 0.73 -2.0 4,115,889 + glpF b3927 0.73 -2.4 4,115,893 - glpF b3927 0.74 -1.6 4,115,953 + glpF b3927 0.81 -1.6 4,115,956 + glpF b3927 0.81 -3.9 4,115,956 + glpF b3927 0.81 -1.7 4,115,960 + glpF b3927 0.82 -3.7 4,115,960 + glpF b3927 0.82 -2.8 4,115,964 - glpF b3927 0.82 -1.0 4,115,966 + glpF b3927 0.83 -3.8 4,115,968 - glpF b3927 0.83 -1.9 4,116,014 + glpF b3927 0.88 -4.1 4,116,023 + glpF b3927 0.89 -2.6 4,116,023 + glpF b3927 0.89 -3.2 4,116,027 + glpF b3927 0.90 -3.5 4,116,036 - -0.8 4,116,056 + -4.0 4,116,081 + -2.0 4,116,101 + -0.9 4,116,101 + -3.8 4,116,103 + -1.6 4,116,138 - -0.7 4,116,148 + -2.5 4,116,171 + -0.2 4,116,174 + -2.6 4,116,175 + -4.7 4,116,176 + -2.2 4,116,193 - -0.9 4,116,236 + -3.8 4,116,236 + -2.9 4,116,236 - -0.7 4,116,236 - -3.0 4,116,241 - -1.9 4,116,241 - +0.9 4,116,289 + -1.3 4,116,289 + +0.0 4,116,292 + +0.2 4,116,293 - -0.8 4,116,355 - -0.4 4,116,360 - -0.2 4,116,381 + +0.2 4,116,398 + +1.3 4,116,415 + +0.2 4,116,415 + -0.1 4,116,415 + -0.9 4,116,418 - -1.4 4,116,420 + +1.0 4,116,459 - +0.0 4,116,528 - +0.6 4,116,533 - -1.3 4,116,534 + -0.5 4,116,535 + -0.1 4,116,542 - -0.1 4,116,543 - -0.6 4,116,543 - +0.6 4,116,548 + +0.7 4,116,548 + +1.3 4,116,548 + -0.3 4,116,564 - yiiU b3928 0.11 -0.2 4,116,580 + yiiU b3928 0.17 +0.4 4,116,592 + yiiU b3928 0.22 +0.3 4,116,597 + yiiU b3928 0.24 -0.3 4,116,599 + yiiU b3928 0.25 -1.3 4,116,599 + yiiU b3928 0.25 +0.2 4,116,603 - yiiU b3928 0.26 +0.3 4,116,607 + yiiU b3928 0.28 -0.6 4,116,647 + yiiU b3928 0.44 -0.0 4,116,647 + yiiU b3928 0.44 +0.3 4,116,654 + yiiU b3928 0.47 -0.5 4,116,683 + yiiU b3928 0.59 -0.2 4,116,686 - yiiU b3928 0.60 -1.2 4,116,686 - yiiU b3928 0.60 +0.9 4,116,691 + yiiU b3928 0.62 -0.4 4,116,691 + yiiU b3928 0.62 +1.0 4,116,748 + yiiU b3928 0.85 +0.4 4,116,767 + -0.3 4,116,767 + -0.8 4,116,767 + -0.3 4,116,775 - +0.6 4,116,782 + -0.1 4,116,782 + -0.9 4,116,785 - -1.1 4,116,789 - -2.4 4,116,789 - +0.4 4,116,816 + -0.1 4,116,816 + +1.0 4,116,816 + +0.1 4,116,824 - +0.5 4,116,824 - +0.5 4,116,824 - +0.4 4,116,824 - +0.2 4,116,824 - +0.9 4,116,858 + +1.0 4,116,883 - +0.3 4,116,883 - +0.9 4,116,930 + rraA b3929 0.13 +1.6 4,116,930 + rraA b3929 0.13 +1.8 4,116,932 - rraA b3929 0.13 +1.3 4,116,932 - rraA b3929 0.13 +1.5 4,116,940 + rraA b3929 0.15 +1.7 4,116,960 - rraA b3929 0.19 +1.8 4,117,029 - rraA b3929 0.33 +2.0 4,117,029 - rraA b3929 0.33 +2.5 4,117,039 - rraA b3929 0.35 +1.6 4,117,050 - rraA b3929 0.37 +1.1 4,117,083 - rraA b3929 0.44 +1.3 4,117,083 - rraA b3929 0.44 +1.1
Or see this region's nucleotide sequence