Strain Fitness in Escherichia coli BW25113 around b3459

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
3,595,035	+	livK	b3458	0.51	-0.0
3,595,043	-	livK	b3458	0.51	-0.5
3,595,053	-	livK	b3458	0.52	+0.2
3,595,097	+	livK	b3458	0.56	-0.8
3,595,109	-	livK	b3458	0.57	-0.0
3,595,142	+	livK	b3458	0.60	-1.8
3,595,199	+	livK	b3458	0.65	-2.4
3,595,207	-	livK	b3458	0.66	-1.7
3,595,207	-	livK	b3458	0.66	-0.4
3,595,207	-	livK	b3458	0.66	-1.9
3,595,264	+	livK	b3458	0.71	-3.4
3,595,276	-	livK	b3458	0.72	-0.7
3,595,343	-	livK	b3458	0.78	-1.0
3,595,343	-	livK	b3458	0.78	-1.6
3,595,354	-	livK	b3458	0.79	-0.4
3,595,365	+	livK	b3458	0.80	-1.8
3,595,427	-	livK	b3458	0.86	-0.6
3,595,466	+	livK	b3458	0.89	-1.5
3,595,466	+	livK	b3458	0.89	-0.3
3,595,510	-				-2.8
3,595,517	+				-1.0
3,595,517	+				-1.6
3,595,549	-				-1.0
3,595,553	-				-0.7
3,595,553	-				-1.6
3,595,554	+				-0.4
3,595,637	+				-1.4
3,595,669	+				-1.2
3,595,968	+				-0.1
3,595,975	+				-1.2
3,596,005	+				+4.8
3,596,005	-				-0.8
3,596,005	-				-1.1
3,596,007	+				-0.1
3,596,011	-				+0.7
3,596,012	-				-0.1
3,596,013	-				-0.2
3,596,043	+				-1.8
3,596,048	+	yhhK	b3459	0.11	+0.9
3,596,048	+	yhhK	b3459	0.11	-0.9
3,596,056	-	yhhK	b3459	0.13	+0.5
3,596,056	-	yhhK	b3459	0.13	-0.6
3,596,061	-	yhhK	b3459	0.14	+1.0
3,596,117	+	yhhK	b3459	0.29	-0.5
3,596,128	-	yhhK	b3459	0.32	-1.6
3,596,163	-	yhhK	b3459	0.41	-1.4
3,596,163	-	yhhK	b3459	0.41	+0.7
3,596,169	+	yhhK	b3459	0.42	-1.7
3,596,209	+	yhhK	b3459	0.53	+1.0
3,596,209	+	yhhK	b3459	0.53	-0.9
3,596,251	-	yhhK	b3459	0.64	-2.0
3,596,251	-	yhhK	b3459	0.64	+0.7
3,596,267	+	yhhK	b3459	0.68	+0.2
3,596,267	+	yhhK	b3459	0.68	-0.5
3,596,288	+	yhhK	b3459	0.73	+0.6
3,596,334	+	yhhK	b3459	0.85	+0.6
3,596,420	+				-0.1
3,596,427	+				+0.7
3,596,427	+				+0.1
3,596,500	+				+0.1
3,596,588	-				+1.0
3,596,657	+				-0.3
3,596,737	+	livJ	b3460	0.14	-0.6
3,596,906	+	livJ	b3460	0.30	+0.1
3,596,914	-	livJ	b3460	0.30	-1.1
3,596,914	-	livJ	b3460	0.30	-0.4
3,596,994	-	livJ	b3460	0.38	-0.8
3,596,994	-	livJ	b3460	0.38	-1.4
3,597,006	+	livJ	b3460	0.39	-1.9
3,597,133	+	livJ	b3460	0.50	-0.3
3,597,141	-	livJ	b3460	0.51	+0.5
3,597,141	-	livJ	b3460	0.51	+0.0
3,597,141	-	livJ	b3460	0.51	+0.1
3,597,141	-	livJ	b3460	0.51	-1.4
3,597,148	+	livJ	b3460	0.52	-0.6
3,597,154	+	livJ	b3460	0.52	+0.2
3,597,154	+	livJ	b3460	0.52	-0.4
3,597,156	-	livJ	b3460	0.52	-0.8
3,597,161	+	livJ	b3460	0.53	-0.4
3,597,188	+	livJ	b3460	0.55	+0.1
3,597,188	+	livJ	b3460	0.55	-0.5
3,597,240	+	livJ	b3460	0.60	-1.2
3,597,240	+	livJ	b3460	0.60	-2.0
3,597,240	-	livJ	b3460	0.60	-0.7
3,597,250	+	livJ	b3460	0.61	-0.9
3,597,250	+	livJ	b3460	0.61	-0.0
3,597,251	-	livJ	b3460	0.61	+0.3
3,597,251	-	livJ	b3460	0.61	+0.1
3,597,251	-	livJ	b3460	0.61	-0.0
3,597,276	+	livJ	b3460	0.63	-0.8
3,597,312	+	livJ	b3460	0.66	-0.7
3,597,312	+	livJ	b3460	0.66	-1.4
3,597,320	-	livJ	b3460	0.67	-1.6
3,597,377	+	livJ	b3460	0.72	-0.1
3,597,377	+	livJ	b3460	0.72	-0.6
3,597,385	-	livJ	b3460	0.73	+0.1
3,597,385	-	livJ	b3460	0.73	-0.5
3,597,385	-	livJ	b3460	0.73	-1.3
3,597,385	-	livJ	b3460	0.73	-0.3