Strain Fitness in Escherichia coli BW25113 around b3457

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlivM and livH overlap by 4 nucleotideslivH and livK are separated by 47 nucleotides b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503 livM b3457: livH - leucine/isoleucine/valine transporter subunit (NCBI), at 3,593,500 to 3,594,426 livH b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583 livK Position (kb) 3593 3594 3595Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3592.590 kb on - strand, within livMat 3592.640 kb on + strand, within livMat 3592.763 kb on + strand, within livMat 3592.763 kb on + strand, within livMat 3592.768 kb on + strand, within livMat 3592.768 kb on + strand, within livMat 3592.822 kb on + strand, within livMat 3592.822 kb on + strand, within livMat 3592.834 kb on + strand, within livMat 3593.081 kb on + strand, within livMat 3593.081 kb on + strand, within livMat 3593.103 kb on - strand, within livMat 3593.103 kb on - strand, within livMat 3593.139 kb on + strand, within livMat 3593.210 kb on + strand, within livMat 3593.210 kb on + strand, within livMat 3593.244 kb on + strand, within livMat 3593.339 kb on + strand, within livMat 3593.375 kb on - strand, within livMat 3593.383 kb on - strandat 3593.383 kb on - strandat 3593.401 kb on + strandat 3593.409 kb on - strandat 3593.424 kb on - strandat 3593.438 kb on - strandat 3593.438 kb on - strandat 3593.524 kb on + strandat 3593.620 kb on - strand, within livHat 3593.620 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.660 kb on + strand, within livHat 3593.660 kb on + strand, within livHat 3593.749 kb on + strand, within livHat 3593.825 kb on + strand, within livHat 3593.833 kb on - strand, within livHat 3593.869 kb on + strand, within livHat 3593.942 kb on + strand, within livHat 3593.942 kb on + strand, within livHat 3593.966 kb on - strand, within livHat 3594.055 kb on - strand, within livHat 3594.098 kb on - strand, within livHat 3594.150 kb on + strand, within livHat 3594.150 kb on + strand, within livHat 3594.220 kb on + strand, within livHat 3594.232 kb on - strand, within livHat 3594.232 kb on - strand, within livHat 3594.236 kb on + strand, within livHat 3594.236 kb on + strand, within livHat 3594.346 kb on - strandat 3594.350 kb on - strandat 3594.350 kb on - strandat 3594.404 kb on - strandat 3594.404 kb on - strandat 3594.417 kb on + strandat 3594.469 kb on + strandat 3594.485 kb on - strandat 3594.518 kb on - strandat 3594.593 kb on + strand, within livKat 3594.638 kb on + strand, within livKat 3594.639 kb on + strand, within livKat 3594.639 kb on + strand, within livKat 3594.639 kb on + strand, within livKat 3594.639 kb on + strand, within livKat 3594.743 kb on - strand, within livKat 3594.821 kb on + strand, within livKat 3594.876 kb on + strand, within livKat 3594.876 kb on + strand, within livKat 3594.908 kb on + strand, within livKat 3594.954 kb on + strand, within livKat 3595.035 kb on + strand, within livKat 3595.043 kb on - strand, within livKat 3595.053 kb on - strand, within livKat 3595.097 kb on + strand, within livKat 3595.109 kb on - strand, within livKat 3595.142 kb on + strand, within livKat 3595.199 kb on + strand, within livKat 3595.207 kb on - strand, within livKat 3595.207 kb on - strand, within livKat 3595.207 kb on - strand, within livKat 3595.264 kb on + strand, within livKat 3595.276 kb on - strand, within livKat 3595.343 kb on - strand, within livKat 3595.343 kb on - strand, within livKat 3595.354 kb on - strand, within livKat 3595.365 kb on + strand, within livK

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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3,592,590 - livM b3456 0.28 -1.2
3,592,640 + livM b3456 0.32 -1.7
3,592,763 + livM b3456 0.42 -0.1
3,592,763 + livM b3456 0.42 -0.0
3,592,768 + livM b3456 0.42 -2.6
3,592,768 + livM b3456 0.42 -2.1
3,592,822 + livM b3456 0.47 -1.2
3,592,822 + livM b3456 0.47 -0.9
3,592,834 + livM b3456 0.48 -1.4
3,593,081 + livM b3456 0.67 -1.3
3,593,081 + livM b3456 0.67 -0.4
3,593,103 - livM b3456 0.69 -3.8
3,593,103 - livM b3456 0.69 -1.8
3,593,139 + livM b3456 0.71 -1.2
3,593,210 + livM b3456 0.77 -1.6
3,593,210 + livM b3456 0.77 -0.9
3,593,244 + livM b3456 0.80 -1.2
3,593,339 + livM b3456 0.87 -1.3
3,593,375 - livM b3456 0.90 -0.6
3,593,383 - -2.3
3,593,383 - -1.5
3,593,401 + -0.6
3,593,409 - -2.3
3,593,424 - -1.6
3,593,438 - -2.6
3,593,438 - -0.9
3,593,524 + -1.3
3,593,620 - livH b3457 0.13 -1.0
3,593,620 - livH b3457 0.13 -0.3
3,593,655 - livH b3457 0.17 -0.7
3,593,655 - livH b3457 0.17 -2.9
3,593,655 - livH b3457 0.17 -2.1
3,593,655 - livH b3457 0.17 -1.1
3,593,660 + livH b3457 0.17 -2.3
3,593,660 + livH b3457 0.17 -1.6
3,593,749 + livH b3457 0.27 -0.5
3,593,825 + livH b3457 0.35 -2.2
3,593,833 - livH b3457 0.36 -0.0
3,593,869 + livH b3457 0.40 -0.9
3,593,942 + livH b3457 0.48 -1.8
3,593,942 + livH b3457 0.48 -3.3
3,593,966 - livH b3457 0.50 -0.6
3,594,055 - livH b3457 0.60 -1.4
3,594,098 - livH b3457 0.65 -2.2
3,594,150 + livH b3457 0.70 -0.4
3,594,150 + livH b3457 0.70 -1.2
3,594,220 + livH b3457 0.78 -1.7
3,594,232 - livH b3457 0.79 -0.5
3,594,232 - livH b3457 0.79 -0.4
3,594,236 + livH b3457 0.79 -0.4
3,594,236 + livH b3457 0.79 -1.0
3,594,346 - -1.3
3,594,350 - +0.3
3,594,350 - -3.2
3,594,404 - -0.4
3,594,404 - -0.7
3,594,417 + -0.8
3,594,469 + -1.3
3,594,485 - +0.8
3,594,518 - -0.2
3,594,593 + livK b3458 0.11 -0.9
3,594,638 + livK b3458 0.15 -0.1
3,594,639 + livK b3458 0.15 -0.9
3,594,639 + livK b3458 0.15 -0.6
3,594,639 + livK b3458 0.15 -0.8
3,594,639 + livK b3458 0.15 -1.8
3,594,743 - livK b3458 0.24 -0.9
3,594,821 + livK b3458 0.31 -2.4
3,594,876 + livK b3458 0.36 +0.3
3,594,876 + livK b3458 0.36 -1.2
3,594,908 + livK b3458 0.39 +1.0
3,594,954 + livK b3458 0.43 -0.2
3,595,035 + livK b3458 0.51 -0.0
3,595,043 - livK b3458 0.51 -0.5
3,595,053 - livK b3458 0.52 +0.2
3,595,097 + livK b3458 0.56 -0.8
3,595,109 - livK b3458 0.57 -0.0
3,595,142 + livK b3458 0.60 -1.8
3,595,199 + livK b3458 0.65 -2.4
3,595,207 - livK b3458 0.66 -1.7
3,595,207 - livK b3458 0.66 -0.4
3,595,207 - livK b3458 0.66 -1.9
3,595,264 + livK b3458 0.71 -3.4
3,595,276 - livK b3458 0.72 -0.7
3,595,343 - livK b3458 0.78 -1.0
3,595,343 - livK b3458 0.78 -1.6
3,595,354 - livK b3458 0.79 -0.4
3,595,365 + livK b3458 0.80 -1.8

Or see this region's nucleotide sequence