Strain Fitness in Escherichia coli BW25113 around b3456

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlivF and livG are separated by 1 nucleotideslivG and livM overlap by 4 nucleotideslivM and livH overlap by 4 nucleotideslivH and livK are separated by 47 nucleotides b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460 livF b3455: livG - leucine/isoleucine/valine transporter subunit (NCBI), at 3,591,462 to 3,592,229 livG b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503 livM b3457: livH - leucine/isoleucine/valine transporter subunit (NCBI), at 3,593,500 to 3,594,426 livH b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583 livK Position (kb) 3592 3593 3594Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3591.252 kb on + strand, within livFat 3591.252 kb on + strand, within livFat 3591.381 kb on - strand, within livFat 3591.381 kb on - strand, within livFat 3591.419 kb on - strandat 3591.479 kb on - strandat 3591.479 kb on - strandat 3591.547 kb on - strand, within livGat 3591.584 kb on + strand, within livGat 3591.592 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.748 kb on - strand, within livGat 3591.765 kb on + strand, within livGat 3591.768 kb on - strand, within livGat 3591.834 kb on + strand, within livGat 3591.834 kb on + strand, within livGat 3591.881 kb on + strand, within livGat 3591.907 kb on + strand, within livGat 3591.937 kb on - strand, within livGat 3591.943 kb on - strand, within livGat 3591.962 kb on - strand, within livGat 3591.974 kb on - strand, within livGat 3592.053 kb on + strand, within livGat 3592.148 kb on - strand, within livGat 3592.229 kb on + strandat 3592.321 kb on - strandat 3592.436 kb on + strand, within livMat 3592.436 kb on + strand, within livMat 3592.590 kb on - strand, within livMat 3592.640 kb on + strand, within livMat 3592.763 kb on + strand, within livMat 3592.763 kb on + strand, within livMat 3592.768 kb on + strand, within livMat 3592.768 kb on + strand, within livMat 3592.822 kb on + strand, within livMat 3592.822 kb on + strand, within livMat 3592.834 kb on + strand, within livMat 3593.081 kb on + strand, within livMat 3593.081 kb on + strand, within livMat 3593.103 kb on - strand, within livMat 3593.103 kb on - strand, within livMat 3593.139 kb on + strand, within livMat 3593.210 kb on + strand, within livMat 3593.210 kb on + strand, within livMat 3593.244 kb on + strand, within livMat 3593.339 kb on + strand, within livMat 3593.375 kb on - strand, within livMat 3593.383 kb on - strandat 3593.383 kb on - strandat 3593.401 kb on + strandat 3593.409 kb on - strandat 3593.424 kb on - strandat 3593.438 kb on - strandat 3593.438 kb on - strandat 3593.524 kb on + strandat 3593.620 kb on - strand, within livHat 3593.620 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.655 kb on - strand, within livHat 3593.660 kb on + strand, within livHat 3593.660 kb on + strand, within livHat 3593.749 kb on + strand, within livHat 3593.825 kb on + strand, within livHat 3593.833 kb on - strand, within livHat 3593.869 kb on + strand, within livHat 3593.942 kb on + strand, within livHat 3593.942 kb on + strand, within livHat 3593.966 kb on - strand, within livHat 3594.055 kb on - strand, within livHat 3594.098 kb on - strand, within livHat 3594.150 kb on + strand, within livHat 3594.150 kb on + strand, within livHat 3594.220 kb on + strand, within livHat 3594.232 kb on - strand, within livHat 3594.232 kb on - strand, within livHat 3594.236 kb on + strand, within livHat 3594.236 kb on + strand, within livHat 3594.346 kb on - strandat 3594.350 kb on - strandat 3594.350 kb on - strandat 3594.404 kb on - strandat 3594.404 kb on - strandat 3594.417 kb on + strandat 3594.469 kb on + strandat 3594.485 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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3,591,252 + livF b3454 0.71 -1.9
3,591,252 + livF b3454 0.71 -2.0
3,591,381 - livF b3454 0.89 -1.8
3,591,381 - livF b3454 0.89 -0.5
3,591,419 - -0.8
3,591,479 - -2.8
3,591,479 - -1.5
3,591,547 - livG b3455 0.11 -1.5
3,591,584 + livG b3455 0.16 +1.5
3,591,592 - livG b3455 0.17 -1.2
3,591,748 - livG b3455 0.37 -1.3
3,591,748 - livG b3455 0.37 -1.2
3,591,748 - livG b3455 0.37 -1.2
3,591,765 + livG b3455 0.39 -1.8
3,591,768 - livG b3455 0.40 -3.9
3,591,834 + livG b3455 0.48 -0.9
3,591,834 + livG b3455 0.48 -1.5
3,591,881 + livG b3455 0.55 +2.1
3,591,907 + livG b3455 0.58 -1.7
3,591,937 - livG b3455 0.62 -3.1
3,591,943 - livG b3455 0.63 -1.6
3,591,962 - livG b3455 0.65 -2.2
3,591,974 - livG b3455 0.67 +0.8
3,592,053 + livG b3455 0.77 -0.4
3,592,148 - livG b3455 0.89 -1.7
3,592,229 + -1.1
3,592,321 - -0.5
3,592,436 + livM b3456 0.16 -1.4
3,592,436 + livM b3456 0.16 -0.6
3,592,590 - livM b3456 0.28 -1.2
3,592,640 + livM b3456 0.32 -1.7
3,592,763 + livM b3456 0.42 -0.1
3,592,763 + livM b3456 0.42 -0.0
3,592,768 + livM b3456 0.42 -2.6
3,592,768 + livM b3456 0.42 -2.1
3,592,822 + livM b3456 0.47 -1.2
3,592,822 + livM b3456 0.47 -0.9
3,592,834 + livM b3456 0.48 -1.4
3,593,081 + livM b3456 0.67 -1.3
3,593,081 + livM b3456 0.67 -0.4
3,593,103 - livM b3456 0.69 -3.8
3,593,103 - livM b3456 0.69 -1.8
3,593,139 + livM b3456 0.71 -1.2
3,593,210 + livM b3456 0.77 -1.6
3,593,210 + livM b3456 0.77 -0.9
3,593,244 + livM b3456 0.80 -1.2
3,593,339 + livM b3456 0.87 -1.3
3,593,375 - livM b3456 0.90 -0.6
3,593,383 - -2.3
3,593,383 - -1.5
3,593,401 + -0.6
3,593,409 - -2.3
3,593,424 - -1.6
3,593,438 - -2.6
3,593,438 - -0.9
3,593,524 + -1.3
3,593,620 - livH b3457 0.13 -1.0
3,593,620 - livH b3457 0.13 -0.3
3,593,655 - livH b3457 0.17 -0.7
3,593,655 - livH b3457 0.17 -2.9
3,593,655 - livH b3457 0.17 -2.1
3,593,655 - livH b3457 0.17 -1.1
3,593,660 + livH b3457 0.17 -2.3
3,593,660 + livH b3457 0.17 -1.6
3,593,749 + livH b3457 0.27 -0.5
3,593,825 + livH b3457 0.35 -2.2
3,593,833 - livH b3457 0.36 -0.0
3,593,869 + livH b3457 0.40 -0.9
3,593,942 + livH b3457 0.48 -1.8
3,593,942 + livH b3457 0.48 -3.3
3,593,966 - livH b3457 0.50 -0.6
3,594,055 - livH b3457 0.60 -1.4
3,594,098 - livH b3457 0.65 -2.2
3,594,150 + livH b3457 0.70 -0.4
3,594,150 + livH b3457 0.70 -1.2
3,594,220 + livH b3457 0.78 -1.7
3,594,232 - livH b3457 0.79 -0.5
3,594,232 - livH b3457 0.79 -0.4
3,594,236 + livH b3457 0.79 -0.4
3,594,236 + livH b3457 0.79 -1.0
3,594,346 - -1.3
3,594,350 - +0.3
3,594,350 - -3.2
3,594,404 - -0.4
3,594,404 - -0.7
3,594,417 + -0.8
3,594,469 + -1.3
3,594,485 - +0.8

Or see this region's nucleotide sequence