Strain Fitness in Escherichia coli BW25113 around b2218

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrcsD and rcsB are separated by 16 nucleotidesrcsB and rcsC are separated by 199 nucleotidesrcsC and atoS are separated by 166 nucleotides b2216: rcsD - phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI), at 2,311,510 to 2,314,182 rcsD b2217: rcsB - DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI), at 2,314,199 to 2,314,849 rcsB b2218: rcsC - hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq), at 2,315,049 to 2,317,898 rcsC b2219: atoS - sensory histidine kinase in two-component regulatory system with AtoC (NCBI), at 2,318,065 to 2,319,891 atoS Position (kb) 2315 2316 2317 2318Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 2314.102 kb on + strandat 2314.202 kb on - strandat 2314.311 kb on + strand, within rcsBat 2314.319 kb on + strand, within rcsBat 2314.323 kb on + strand, within rcsBat 2314.362 kb on - strand, within rcsBat 2314.362 kb on - strand, within rcsBat 2314.439 kb on - strand, within rcsBat 2314.439 kb on - strand, within rcsBat 2314.490 kb on + strand, within rcsBat 2314.490 kb on + strand, within rcsBat 2314.565 kb on + strand, within rcsBat 2314.599 kb on + strand, within rcsBat 2314.773 kb on + strand, within rcsBat 2314.812 kb on - strandat 2315.032 kb on - strandat 2315.045 kb on - strandat 2315.139 kb on + strandat 2315.341 kb on + strand, within rcsCat 2315.548 kb on + strand, within rcsCat 2315.559 kb on - strand, within rcsCat 2315.678 kb on + strand, within rcsCat 2315.702 kb on + strand, within rcsCat 2315.745 kb on + strand, within rcsCat 2315.782 kb on + strand, within rcsCat 2315.785 kb on - strand, within rcsCat 2315.785 kb on - strand, within rcsCat 2315.875 kb on - strand, within rcsCat 2315.875 kb on - strand, within rcsCat 2315.894 kb on + strand, within rcsCat 2315.894 kb on + strand, within rcsCat 2315.947 kb on + strand, within rcsCat 2315.994 kb on - strand, within rcsCat 2316.032 kb on + strand, within rcsCat 2316.095 kb on - strand, within rcsCat 2316.101 kb on + strand, within rcsCat 2316.101 kb on + strand, within rcsCat 2316.127 kb on + strand, within rcsCat 2316.130 kb on - strand, within rcsCat 2316.130 kb on - strand, within rcsCat 2316.249 kb on + strand, within rcsCat 2316.264 kb on - strand, within rcsCat 2316.286 kb on + strand, within rcsCat 2316.294 kb on - strand, within rcsCat 2316.381 kb on - strand, within rcsCat 2316.542 kb on - strand, within rcsCat 2316.695 kb on + strand, within rcsCat 2316.703 kb on - strand, within rcsCat 2316.726 kb on - strand, within rcsCat 2316.726 kb on - strand, within rcsCat 2316.775 kb on - strand, within rcsCat 2316.783 kb on - strand, within rcsCat 2316.909 kb on - strand, within rcsCat 2316.977 kb on - strand, within rcsCat 2317.003 kb on + strand, within rcsCat 2317.012 kb on + strand, within rcsCat 2317.046 kb on + strand, within rcsCat 2317.051 kb on - strand, within rcsCat 2317.181 kb on + strand, within rcsCat 2317.315 kb on + strand, within rcsCat 2317.395 kb on + strand, within rcsCat 2317.416 kb on - strand, within rcsCat 2317.538 kb on - strand, within rcsCat 2317.589 kb on + strand, within rcsCat 2317.635 kb on - strandat 2317.682 kb on + strandat 2317.818 kb on - strandat 2317.822 kb on - strandat 2317.931 kb on + strandat 2317.936 kb on + strandat 2318.000 kb on - strandat 2318.000 kb on - strandat 2318.067 kb on + strandat 2318.114 kb on + strandat 2318.128 kb on - strandat 2318.163 kb on - strandat 2318.189 kb on - strandat 2318.244 kb on + strandat 2318.265 kb on - strand, within atoSat 2318.371 kb on - strand, within atoSat 2318.461 kb on - strand, within atoSat 2318.462 kb on - strand, within atoSat 2318.473 kb on - strand, within atoSat 2318.473 kb on - strand, within atoSat 2318.473 kb on - strand, within atoSat 2318.514 kb on + strand, within atoSat 2318.514 kb on + strand, within atoSat 2318.558 kb on + strand, within atoSat 2318.558 kb on + strand, within atoSat 2318.610 kb on + strand, within atoSat 2318.664 kb on + strand, within atoSat 2318.672 kb on - strand, within atoSat 2318.810 kb on + strand, within atoSat 2318.827 kb on - strand, within atoS

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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2,314,102 + -0.3
2,314,202 - -0.7
2,314,311 + rcsB b2217 0.17 -0.0
2,314,319 + rcsB b2217 0.18 -1.1
2,314,323 + rcsB b2217 0.19 -0.8
2,314,362 - rcsB b2217 0.25 -1.4
2,314,362 - rcsB b2217 0.25 -1.3
2,314,439 - rcsB b2217 0.37 -0.9
2,314,439 - rcsB b2217 0.37 -2.1
2,314,490 + rcsB b2217 0.45 -3.1
2,314,490 + rcsB b2217 0.45 -4.2
2,314,565 + rcsB b2217 0.56 -0.1
2,314,599 + rcsB b2217 0.61 -0.0
2,314,773 + rcsB b2217 0.88 -0.8
2,314,812 - -2.4
2,315,032 - +0.3
2,315,045 - +0.7
2,315,139 + -0.6
2,315,341 + rcsC b2218 0.10 +2.5
2,315,548 + rcsC b2218 0.18 +2.3
2,315,559 - rcsC b2218 0.18 +2.6
2,315,678 + rcsC b2218 0.22 +4.1
2,315,702 + rcsC b2218 0.23 +1.4
2,315,745 + rcsC b2218 0.24 +2.1
2,315,782 + rcsC b2218 0.26 +1.4
2,315,785 - rcsC b2218 0.26 +2.7
2,315,785 - rcsC b2218 0.26 +3.0
2,315,875 - rcsC b2218 0.29 +2.5
2,315,875 - rcsC b2218 0.29 +2.2
2,315,894 + rcsC b2218 0.30 +4.3
2,315,894 + rcsC b2218 0.30 +3.1
2,315,947 + rcsC b2218 0.32 +2.8
2,315,994 - rcsC b2218 0.33 +2.2
2,316,032 + rcsC b2218 0.34 +2.4
2,316,095 - rcsC b2218 0.37 +2.0
2,316,101 + rcsC b2218 0.37 +1.3
2,316,101 + rcsC b2218 0.37 +2.2
2,316,127 + rcsC b2218 0.38 +1.9
2,316,130 - rcsC b2218 0.38 +1.6
2,316,130 - rcsC b2218 0.38 +2.1
2,316,249 + rcsC b2218 0.42 +1.8
2,316,264 - rcsC b2218 0.43 +1.6
2,316,286 + rcsC b2218 0.43 +3.7
2,316,294 - rcsC b2218 0.44 +3.1
2,316,381 - rcsC b2218 0.47 +3.2
2,316,542 - rcsC b2218 0.52 +1.9
2,316,695 + rcsC b2218 0.58 +1.9
2,316,703 - rcsC b2218 0.58 +2.0
2,316,726 - rcsC b2218 0.59 +2.9
2,316,726 - rcsC b2218 0.59 +3.0
2,316,775 - rcsC b2218 0.61 +2.0
2,316,783 - rcsC b2218 0.61 +3.7
2,316,909 - rcsC b2218 0.65 +3.9
2,316,977 - rcsC b2218 0.68 +0.1
2,317,003 + rcsC b2218 0.69 +3.1
2,317,012 + rcsC b2218 0.69 +1.7
2,317,046 + rcsC b2218 0.70 +2.4
2,317,051 - rcsC b2218 0.70 +1.8
2,317,181 + rcsC b2218 0.75 +2.6
2,317,315 + rcsC b2218 0.80 +2.9
2,317,395 + rcsC b2218 0.82 +2.3
2,317,416 - rcsC b2218 0.83 +4.1
2,317,538 - rcsC b2218 0.87 +3.2
2,317,589 + rcsC b2218 0.89 +1.5
2,317,635 - +3.8
2,317,682 + +2.3
2,317,818 - +2.8
2,317,822 - +3.4
2,317,931 + +0.8
2,317,936 + -0.0
2,318,000 - +0.2
2,318,000 - -1.4
2,318,067 + +0.2
2,318,114 + +0.1
2,318,128 - -0.5
2,318,163 - +1.1
2,318,189 - -0.7
2,318,244 + -0.8
2,318,265 - atoS b2219 0.11 -2.1
2,318,371 - atoS b2219 0.17 -0.7
2,318,461 - atoS b2219 0.22 -0.4
2,318,462 - atoS b2219 0.22 +0.7
2,318,473 - atoS b2219 0.22 -2.2
2,318,473 - atoS b2219 0.22 +0.8
2,318,473 - atoS b2219 0.22 -0.9
2,318,514 + atoS b2219 0.25 -0.5
2,318,514 + atoS b2219 0.25 +1.2
2,318,558 + atoS b2219 0.27 -0.7
2,318,558 + atoS b2219 0.27 -0.4
2,318,610 + atoS b2219 0.30 -1.8
2,318,664 + atoS b2219 0.33 -0.3
2,318,672 - atoS b2219 0.33 +0.0
2,318,810 + atoS b2219 0.41 -0.7
2,318,827 - atoS b2219 0.42 +0.1

Or see this region's nucleotide sequence