Strain Fitness in Escherichia coli BW25113 around b1193

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldcA and mltE are separated by 99 nucleotidesmltE and ycgR are separated by 1 nucleotidesycgR and ymgE are separated by 200 nucleotides b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303 ldcA b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014 mltE b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750 ycgR b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205 ymgE Position (kb) 1242 1243 1244Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1241.424 kb on + strandat 1241.425 kb on - strandat 1241.433 kb on + strandat 1241.501 kb on + strand, within ldcAat 1241.572 kb on - strand, within ldcAat 1241.581 kb on + strand, within ldcAat 1241.747 kb on + strand, within ldcAat 1241.936 kb on - strand, within ldcAat 1241.936 kb on - strand, within ldcAat 1241.961 kb on - strand, within ldcAat 1241.961 kb on - strand, within ldcAat 1241.999 kb on - strand, within ldcAat 1242.033 kb on + strand, within ldcAat 1242.208 kb on - strand, within ldcAat 1242.237 kb on - strandat 1242.284 kb on + strandat 1242.305 kb on - strandat 1242.337 kb on + strandat 1242.355 kb on - strandat 1242.365 kb on - strandat 1242.365 kb on - strandat 1242.465 kb on + strand, within mltEat 1242.655 kb on + strand, within mltEat 1243.159 kb on + strand, within ycgRat 1243.291 kb on - strand, within ycgRat 1243.291 kb on - strand, within ycgRat 1243.521 kb on + strand, within ycgRat 1243.589 kb on + strand, within ycgRat 1243.637 kb on - strand, within ycgRat 1243.688 kb on + strandat 1243.722 kb on - strandat 1243.732 kb on + strandat 1243.798 kb on + strandat 1243.864 kb on + strandat 1243.875 kb on + strandat 1243.890 kb on + strandat 1243.893 kb on - strandat 1243.899 kb on + strandat 1243.904 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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1,241,424 + -4.9
1,241,425 - -5.7
1,241,433 + -6.7
1,241,501 + ldcA b1192 0.12 -5.7
1,241,572 - ldcA b1192 0.20 -6.3
1,241,581 + ldcA b1192 0.21 -5.0
1,241,747 + ldcA b1192 0.39 -3.8
1,241,936 - ldcA b1192 0.60 -6.8
1,241,936 - ldcA b1192 0.60 -7.7
1,241,961 - ldcA b1192 0.63 -6.3
1,241,961 - ldcA b1192 0.63 -6.1
1,241,999 - ldcA b1192 0.67 -5.5
1,242,033 + ldcA b1192 0.70 -6.6
1,242,208 - ldcA b1192 0.90 -4.3
1,242,237 - -6.2
1,242,284 + -3.9
1,242,305 - -4.8
1,242,337 + -0.8
1,242,355 - -0.4
1,242,365 - +0.9
1,242,365 - -1.7
1,242,465 + mltE b1193 0.10 -2.7
1,242,655 + mltE b1193 0.41 +0.9
1,243,159 + ycgR b1194 0.19 +0.2
1,243,291 - ycgR b1194 0.37 -1.4
1,243,291 - ycgR b1194 0.37 -0.8
1,243,521 + ycgR b1194 0.69 +0.4
1,243,589 + ycgR b1194 0.78 +0.4
1,243,637 - ycgR b1194 0.84 -1.6
1,243,688 + -0.1
1,243,722 - +1.6
1,243,732 + +0.9
1,243,798 + -0.1
1,243,864 + +0.3
1,243,875 + +0.9
1,243,890 + +0.8
1,243,893 - -1.1
1,243,899 + +0.3
1,243,904 + +0.3

Or see this region's nucleotide sequence