Strain Fitness in Escherichia coli BW25113 around b2743

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnlpD and pcm are separated by 139 nucleotidespcm and surE overlap by 7 nucleotidessurE and truD overlap by 20 nucleotides b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775 nlpD b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541 pcm b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296 surE b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326 truD Position (kb) 2866 2867 2868Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2866.116 kb on + strand, within nlpDat 2866.116 kb on + strand, within nlpDat 2866.149 kb on + strand, within nlpDat 2866.172 kb on + strand, within nlpDat 2866.210 kb on + strand, within nlpDat 2866.212 kb on + strand, within nlpDat 2866.265 kb on - strand, within nlpDat 2866.380 kb on + strand, within nlpDat 2866.380 kb on + strand, within nlpDat 2866.414 kb on - strand, within nlpDat 2866.414 kb on - strand, within nlpDat 2866.436 kb on + strand, within nlpDat 2866.441 kb on + strand, within nlpDat 2866.463 kb on - strand, within nlpDat 2866.463 kb on - strand, within nlpDat 2866.511 kb on - strand, within nlpDat 2866.511 kb on - strand, within nlpDat 2866.658 kb on + strand, within nlpDat 2866.722 kb on - strandat 2866.836 kb on - strandat 2866.998 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.359 kb on - strand, within pcmat 2867.541 kb on - strandat 2867.541 kb on - strandat 2867.616 kb on - strand, within surEat 2867.623 kb on + strand, within surEat 2867.631 kb on + strand, within surEat 2867.672 kb on - strand, within surEat 2867.701 kb on + strand, within surEat 2867.709 kb on - strand, within surEat 2867.747 kb on + strand, within surEat 2867.747 kb on + strand, within surEat 2867.862 kb on - strand, within surEat 2867.862 kb on - strand, within surEat 2867.862 kb on - strand, within surEat 2867.883 kb on + strand, within surEat 2867.883 kb on + strand, within surEat 2868.038 kb on - strand, within surEat 2868.043 kb on - strand, within surEat 2868.043 kb on - strand, within surEat 2868.152 kb on + strand, within surEat 2868.266 kb on - strandat 2868.284 kb on + strandat 2868.332 kb on - strandat 2868.421 kb on - strand, within truD

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
remove
2,866,116 + nlpD b2742 0.42 +3.5
2,866,116 + nlpD b2742 0.42 +3.1
2,866,149 + nlpD b2742 0.45 +2.7
2,866,172 + nlpD b2742 0.47 -0.6
2,866,210 + nlpD b2742 0.50 +0.2
2,866,212 + nlpD b2742 0.51 -0.1
2,866,265 - nlpD b2742 0.55 +0.3
2,866,380 + nlpD b2742 0.65 +1.5
2,866,380 + nlpD b2742 0.65 -1.8
2,866,414 - nlpD b2742 0.68 -0.1
2,866,414 - nlpD b2742 0.68 +0.2
2,866,436 + nlpD b2742 0.70 -0.2
2,866,441 + nlpD b2742 0.71 -0.2
2,866,463 - nlpD b2742 0.73 +0.5
2,866,463 - nlpD b2742 0.73 +0.1
2,866,511 - nlpD b2742 0.77 -0.6
2,866,511 - nlpD b2742 0.77 -0.8
2,866,658 + nlpD b2742 0.90 -0.7
2,866,722 - +0.3
2,866,836 - +0.6
2,866,998 + pcm b2743 0.13 -0.1
2,867,354 + pcm b2743 0.70 +0.6
2,867,354 + pcm b2743 0.70 +0.2
2,867,354 + pcm b2743 0.70 +0.1
2,867,354 + pcm b2743 0.70 +0.5
2,867,354 + pcm b2743 0.70 -0.6
2,867,359 - pcm b2743 0.71 +0.2
2,867,541 - +0.5
2,867,541 - +0.3
2,867,616 - surE b2744 0.11 -1.1
2,867,623 + surE b2744 0.12 -0.3
2,867,631 + surE b2744 0.13 +0.4
2,867,672 - surE b2744 0.18 -0.1
2,867,701 + surE b2744 0.22 -3.8
2,867,709 - surE b2744 0.23 -1.1
2,867,747 + surE b2744 0.28 -1.2
2,867,747 + surE b2744 0.28 +0.3
2,867,862 - surE b2744 0.43 -0.3
2,867,862 - surE b2744 0.43 -0.5
2,867,862 - surE b2744 0.43 +0.3
2,867,883 + surE b2744 0.46 +0.8
2,867,883 + surE b2744 0.46 -0.8
2,868,038 - surE b2744 0.66 -0.9
2,868,043 - surE b2744 0.67 -0.1
2,868,043 - surE b2744 0.67 +0.1
2,868,152 + surE b2744 0.81 +0.8
2,868,266 - -0.5
2,868,284 + -1.0
2,868,332 - +0.8
2,868,421 - truD b2745 0.14 +0.2

Or see this region's nucleotide sequence