Strain Fitness in Escherichia coli BW25113 around b1725

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpfkB and ydiZ are separated by 100 nucleotidesydiZ and yniA are separated by 105 nucleotidesyniA and yniB are separated by 40 nucleotidesyniB and yniC are separated by 146 nucleotides b1723: pfkB - 6-phosphofructokinase II; suppressor of pfkA (VIMSS), at 1,804,394 to 1,805,323 pfkB b1724: ydiZ - hypothetical protein (NCBI), at 1,805,424 to 1,805,714 ydiZ b1725: yniA - predicted phosphotransferase/kinase (NCBI), at 1,805,820 to 1,806,680 yniA b1726: yniB - predicted inner membrane protein (NCBI), at 1,806,721 to 1,807,257 yniB b1727: yniC - predicted hydrolase (NCBI), at 1,807,404 to 1,808,072 yniC Position (kb) 1805 1806 1807Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 1804.911 kb on + strand, within pfkBat 1804.911 kb on + strand, within pfkBat 1804.911 kb on + strand, within pfkBat 1805.063 kb on + strand, within pfkBat 1805.063 kb on + strand, within pfkBat 1805.077 kb on - strand, within pfkBat 1805.136 kb on - strand, within pfkBat 1805.145 kb on + strand, within pfkBat 1805.355 kb on + strandat 1805.471 kb on - strand, within ydiZat 1805.648 kb on - strand, within ydiZat 1805.853 kb on + strandat 1805.863 kb on - strandat 1805.863 kb on - strandat 1805.980 kb on - strand, within yniAat 1806.165 kb on - strand, within yniAat 1806.217 kb on - strand, within yniAat 1806.333 kb on + strand, within yniAat 1806.412 kb on + strand, within yniAat 1806.412 kb on + strand, within yniAat 1806.430 kb on - strand, within yniAat 1806.458 kb on + strand, within yniAat 1806.458 kb on + strand, within yniAat 1806.464 kb on + strand, within yniAat 1806.563 kb on + strand, within yniAat 1806.563 kb on + strand, within yniAat 1806.573 kb on + strand, within yniAat 1806.593 kb on - strand, within yniAat 1806.593 kb on - strand, within yniAat 1806.598 kb on - strandat 1806.599 kb on - strandat 1806.599 kb on - strandat 1806.665 kb on + strandat 1806.684 kb on + strandat 1806.684 kb on + strandat 1806.686 kb on - strandat 1806.686 kb on - strandat 1806.687 kb on - strandat 1806.697 kb on + strandat 1806.772 kb on - strandat 1806.781 kb on + strand, within yniBat 1807.061 kb on + strand, within yniBat 1807.103 kb on - strand, within yniBat 1807.241 kb on + strandat 1807.253 kb on + strandat 1807.288 kb on + strandat 1807.353 kb on + strandat 1807.363 kb on + strandat 1807.387 kb on - strandat 1807.387 kb on - strandat 1807.470 kb on + strandat 1807.489 kb on + strand, within yniC

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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1,804,911 + pfkB b1723 0.56 +0.3
1,804,911 + pfkB b1723 0.56 +0.2
1,804,911 + pfkB b1723 0.56 +0.0
1,805,063 + pfkB b1723 0.72 +1.4
1,805,063 + pfkB b1723 0.72 +0.2
1,805,077 - pfkB b1723 0.73 +0.3
1,805,136 - pfkB b1723 0.80 -0.2
1,805,145 + pfkB b1723 0.81 -2.5
1,805,355 + -2.6
1,805,471 - ydiZ b1724 0.16 +0.2
1,805,648 - ydiZ b1724 0.77 +1.0
1,805,853 + +0.9
1,805,863 - -1.9
1,805,863 - -2.2
1,805,980 - yniA b1725 0.19 +1.0
1,806,165 - yniA b1725 0.40 +0.9
1,806,217 - yniA b1725 0.46 +0.3
1,806,333 + yniA b1725 0.60 +0.6
1,806,412 + yniA b1725 0.69 +0.5
1,806,412 + yniA b1725 0.69 -1.3
1,806,430 - yniA b1725 0.71 +0.5
1,806,458 + yniA b1725 0.74 +1.2
1,806,458 + yniA b1725 0.74 +0.6
1,806,464 + yniA b1725 0.75 +0.2
1,806,563 + yniA b1725 0.86 +1.1
1,806,563 + yniA b1725 0.86 +0.1
1,806,573 + yniA b1725 0.87 +0.8
1,806,593 - yniA b1725 0.90 +6.0
1,806,593 - yniA b1725 0.90 +5.7
1,806,598 - +1.0
1,806,599 - +1.2
1,806,599 - +0.4
1,806,665 + +0.6
1,806,684 + -0.9
1,806,684 + -0.1
1,806,686 - -0.3
1,806,686 - +0.2
1,806,687 - -0.7
1,806,697 + -1.1
1,806,772 - -0.8
1,806,781 + yniB b1726 0.11 +1.2
1,807,061 + yniB b1726 0.63 -0.3
1,807,103 - yniB b1726 0.71 +0.2
1,807,241 + -0.8
1,807,253 + +0.2
1,807,288 + +0.4
1,807,353 + +0.3
1,807,363 + +1.0
1,807,387 - -0.2
1,807,387 - +0.1
1,807,470 + -0.5
1,807,489 + yniC b1727 0.13 +0.1

Or see this region's nucleotide sequence