Strain Fitness in Escherichia coli BW25113 around b1284

Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrF and yciH overlap by 1 nucleotidesyciH and osmB are separated by 125 nucleotidesosmB and yciT are separated by 268 nucleotidesyciT and yciZ are separated by 89 nucleotidesyciZ and gmr are separated by 147 nucleotides b1281: pyrF - orotidine 5'-phosphate decarboxylase (NCBI), at 1,339,945 to 1,340,682 pyrF b1282: yciH - orf, hypothetical protein (VIMSS), at 1,340,682 to 1,341,008 yciH b1283: osmB - lipoprotein (NCBI), at 1,341,134 to 1,341,352 osmB b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370 yciT b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633 yciZ b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766 gmr Position (kb) 1341 1342 1343Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1340.691 kb on + strandat 1340.708 kb on - strandat 1340.708 kb on - strandat 1340.748 kb on + strand, within yciHat 1340.781 kb on - strand, within yciHat 1340.902 kb on + strand, within yciHat 1340.937 kb on + strand, within yciHat 1340.940 kb on + strand, within yciHat 1340.986 kb on + strandat 1341.128 kb on - strandat 1341.179 kb on - strand, within osmBat 1341.179 kb on - strand, within osmBat 1341.187 kb on - strand, within osmBat 1341.191 kb on - strand, within osmBat 1341.191 kb on - strand, within osmBat 1341.191 kb on - strand, within osmBat 1341.203 kb on - strand, within osmBat 1341.211 kb on + strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.214 kb on - strand, within osmBat 1341.219 kb on - strand, within osmBat 1341.219 kb on - strand, within osmBat 1341.219 kb on - strand, within osmBat 1341.219 kb on - strand, within osmBat 1341.219 kb on - strand, within osmBat 1341.223 kb on - strand, within osmBat 1341.229 kb on - strand, within osmBat 1341.274 kb on + strand, within osmBat 1341.274 kb on + strand, within osmBat 1341.282 kb on - strand, within osmBat 1341.288 kb on + strand, within osmBat 1341.293 kb on - strand, within osmBat 1341.296 kb on - strand, within osmBat 1341.296 kb on - strand, within osmBat 1341.296 kb on - strand, within osmBat 1341.296 kb on - strand, within osmBat 1341.369 kb on - strandat 1341.369 kb on - strandat 1341.400 kb on - strandat 1341.421 kb on - strandat 1341.459 kb on - strandat 1341.465 kb on - strandat 1341.473 kb on - strandat 1341.473 kb on - strandat 1341.483 kb on - strandat 1341.494 kb on - strandat 1341.495 kb on - strandat 1341.495 kb on - strandat 1341.543 kb on - strandat 1341.585 kb on - strandat 1341.585 kb on - strandat 1341.609 kb on - strandat 1341.632 kb on - strandat 1341.645 kb on - strandat 1341.654 kb on + strandat 1341.654 kb on + strandat 1341.656 kb on + strandat 1341.719 kb on + strand, within yciTat 1341.719 kb on + strand, within yciTat 1341.725 kb on - strand, within yciTat 1341.768 kb on + strand, within yciTat 1341.823 kb on - strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.216 kb on - strand, within yciTat 1342.306 kb on - strandat 1342.313 kb on + strandat 1342.313 kb on + strandat 1342.329 kb on + strandat 1342.478 kb on + strand, within yciZat 1342.478 kb on + strand, within yciZat 1342.708 kb on - strandat 1342.809 kb on - strandat 1343.191 kb on - strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.235 kb on + strand, within gmr

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Per-strain Table

Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
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1,340,691 + +0.2
1,340,708 - -0.8
1,340,708 - -1.2
1,340,748 + yciH b1282 0.20 -0.5
1,340,781 - yciH b1282 0.30 +1.2
1,340,902 + yciH b1282 0.67 -1.3
1,340,937 + yciH b1282 0.78 -0.1
1,340,940 + yciH b1282 0.79 -0.2
1,340,986 + -0.3
1,341,128 - -0.5
1,341,179 - osmB b1283 0.21 +0.6
1,341,179 - osmB b1283 0.21 -1.6
1,341,187 - osmB b1283 0.24 -1.6
1,341,191 - osmB b1283 0.26 +0.7
1,341,191 - osmB b1283 0.26 -2.8
1,341,191 - osmB b1283 0.26 +0.0
1,341,203 - osmB b1283 0.32 +0.2
1,341,211 + osmB b1283 0.35 +1.2
1,341,214 - osmB b1283 0.37 -1.1
1,341,214 - osmB b1283 0.37 -0.4
1,341,214 - osmB b1283 0.37 +0.4
1,341,214 - osmB b1283 0.37 -2.1
1,341,214 - osmB b1283 0.37 -0.8
1,341,214 - osmB b1283 0.37 -1.2
1,341,219 - osmB b1283 0.39 -1.3
1,341,219 - osmB b1283 0.39 +0.4
1,341,219 - osmB b1283 0.39 -0.4
1,341,219 - osmB b1283 0.39 +1.0
1,341,219 - osmB b1283 0.39 -0.2
1,341,223 - osmB b1283 0.41 +0.1
1,341,229 - osmB b1283 0.43 -0.4
1,341,274 + osmB b1283 0.64 +1.2
1,341,274 + osmB b1283 0.64 +1.2
1,341,282 - osmB b1283 0.68 +0.3
1,341,288 + osmB b1283 0.70 +1.2
1,341,293 - osmB b1283 0.73 -0.2
1,341,296 - osmB b1283 0.74 +1.0
1,341,296 - osmB b1283 0.74 -2.6
1,341,296 - osmB b1283 0.74 -0.3
1,341,296 - osmB b1283 0.74 +1.5
1,341,369 - -0.3
1,341,369 - -1.5
1,341,400 - +0.3
1,341,421 - +0.6
1,341,459 - +0.0
1,341,465 - -0.9
1,341,473 - +0.1
1,341,473 - +0.8
1,341,483 - -0.6
1,341,494 - +0.1
1,341,495 - -0.6
1,341,495 - -0.4
1,341,543 - -0.8
1,341,585 - +0.1
1,341,585 - -0.1
1,341,609 - +0.2
1,341,632 - +1.9
1,341,645 - +2.8
1,341,654 + +3.8
1,341,654 + -2.1
1,341,656 + +1.2
1,341,719 + yciT b1284 0.13 +1.7
1,341,719 + yciT b1284 0.13 +1.2
1,341,725 - yciT b1284 0.14 +2.8
1,341,768 + yciT b1284 0.20 +2.5
1,341,823 - yciT b1284 0.27 +2.7
1,342,104 + yciT b1284 0.64 +1.7
1,342,104 + yciT b1284 0.64 +1.6
1,342,216 - yciT b1284 0.79 +2.7
1,342,306 - +2.8
1,342,313 + +2.7
1,342,313 + +0.7
1,342,329 + +1.9
1,342,478 + yciZ b4596 0.10 +0.8
1,342,478 + yciZ b4596 0.10 +3.0
1,342,708 - +0.5
1,342,809 - -1.9
1,343,191 - gmr b1285 0.21 -1.5
1,343,224 + gmr b1285 0.22 -1.3
1,343,224 + gmr b1285 0.22 -1.6
1,343,235 + gmr b1285 0.23 -0.1

Or see this region's nucleotide sequence