Experiment: LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpB and dapA are separated by 16 nucleotides dapA and gcvR are separated by 145 nucleotides gcvR and bcp overlap by 1 nucleotides
b2477: nlpB - lipoprotein-34 (VIMSS), at 2,595,853 to 2,596,887
nlpB
b2478: dapA - dihydrodipicolinate synthase (NCBI), at 2,596,904 to 2,597,782
dapA
b2479: gcvR - transcriptional regulation of gcv operon (VIMSS), at 2,597,928 to 2,598,500
gcvR
b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970
bcp
Position (kb)
2596
2597
2598 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2595.937 kb on + strand at 2595.937 kb on + strand at 2595.944 kb on - strand at 2595.944 kb on - strand at 2595.991 kb on + strand, within nlpB at 2595.991 kb on + strand, within nlpB at 2596.206 kb on + strand, within nlpB at 2596.295 kb on - strand, within nlpB at 2596.349 kb on - strand, within nlpB at 2596.439 kb on - strand, within nlpB at 2596.441 kb on + strand, within nlpB at 2596.792 kb on + strand at 2596.792 kb on + strand at 2596.883 kb on - strand at 2596.883 kb on - strand at 2597.909 kb on + strand at 2597.917 kb on - strand at 2597.917 kb on - strand at 2597.962 kb on - strand at 2597.988 kb on - strand, within gcvR at 2598.033 kb on + strand, within gcvR at 2598.054 kb on + strand, within gcvR at 2598.128 kb on - strand, within gcvR at 2598.129 kb on - strand, within gcvR at 2598.129 kb on - strand, within gcvR at 2598.132 kb on + strand, within gcvR at 2598.132 kb on + strand, within gcvR at 2598.139 kb on - strand, within gcvR at 2598.142 kb on + strand, within gcvR at 2598.212 kb on + strand, within gcvR at 2598.282 kb on + strand, within gcvR at 2598.285 kb on + strand, within gcvR at 2598.303 kb on - strand, within gcvR at 2598.303 kb on - strand, within gcvR at 2598.346 kb on - strand, within gcvR at 2598.400 kb on - strand, within gcvR at 2598.400 kb on - strand, within gcvR at 2598.418 kb on - strand, within gcvR at 2598.427 kb on - strand, within gcvR at 2598.513 kb on + strand at 2598.621 kb on + strand, within bcp at 2598.621 kb on + strand, within bcp at 2598.628 kb on - strand, within bcp at 2598.639 kb on - strand, within bcp at 2598.644 kb on - strand, within bcp at 2598.714 kb on - strand, within bcp at 2598.757 kb on + strand, within bcp
Per-strain Table
Position Strand Gene LocusTag Fraction LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) remove 2,595,937 + -0.8 2,595,937 + +0.3 2,595,944 - +0.6 2,595,944 - -1.4 2,595,991 + nlpB b2477 0.13 -0.0 2,595,991 + nlpB b2477 0.13 -0.0 2,596,206 + nlpB b2477 0.34 +0.5 2,596,295 - nlpB b2477 0.43 -1.1 2,596,349 - nlpB b2477 0.48 +0.5 2,596,439 - nlpB b2477 0.57 -0.5 2,596,441 + nlpB b2477 0.57 +0.2 2,596,792 + +0.7 2,596,792 + -0.2 2,596,883 - -0.2 2,596,883 - +0.3 2,597,909 + -0.3 2,597,917 - -3.8 2,597,917 - -1.6 2,597,962 - -2.6 2,597,988 - gcvR b2479 0.10 -3.5 2,598,033 + gcvR b2479 0.18 -2.2 2,598,054 + gcvR b2479 0.22 -2.0 2,598,128 - gcvR b2479 0.35 -2.5 2,598,129 - gcvR b2479 0.35 -1.0 2,598,129 - gcvR b2479 0.35 -2.4 2,598,132 + gcvR b2479 0.36 -3.6 2,598,132 + gcvR b2479 0.36 -3.2 2,598,139 - gcvR b2479 0.37 -3.5 2,598,142 + gcvR b2479 0.37 -1.7 2,598,212 + gcvR b2479 0.50 -1.4 2,598,282 + gcvR b2479 0.62 -3.8 2,598,285 + gcvR b2479 0.62 -2.6 2,598,303 - gcvR b2479 0.65 -2.4 2,598,303 - gcvR b2479 0.65 -2.3 2,598,346 - gcvR b2479 0.73 -1.5 2,598,400 - gcvR b2479 0.82 -3.6 2,598,400 - gcvR b2479 0.82 -2.1 2,598,418 - gcvR b2479 0.86 -1.8 2,598,427 - gcvR b2479 0.87 -3.6 2,598,513 + +1.3 2,598,621 + bcp b2480 0.26 +0.9 2,598,621 + bcp b2480 0.26 +2.3 2,598,628 - bcp b2480 0.27 +0.2 2,598,639 - bcp b2480 0.30 +0.6 2,598,644 - bcp b2480 0.31 +1.3 2,598,714 - bcp b2480 0.45 +0.8 2,598,757 + bcp b2480 0.55 +0.8
Or see this region's nucleotide sequence