Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aldB and yiaY are separated by 164 nucleotides yiaY and selB are separated by 189 nucleotides
b3588: aldB - aldehyde dehydrogenase B (lactaldehyde dehydrogenase) (VIMSS), at 3,752,996 to 3,754,534
aldB
b3589: yiaY - predicted Fe-containing alcohol dehydrogenase (NCBI), at 3,754,699 to 3,755,850
yiaY
b3590: selB - selenocysteinyl-tRNA-specific translation factor (NCBI), at 3,756,040 to 3,757,884
selB
Position (kb)
3754
3755
3756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3753.730 kb on + strand, within aldB at 3753.759 kb on - strand, within aldB at 3753.759 kb on - strand, within aldB at 3753.784 kb on - strand, within aldB at 3753.857 kb on + strand, within aldB at 3753.893 kb on + strand, within aldB at 3753.893 kb on - strand, within aldB at 3753.901 kb on - strand, within aldB at 3753.937 kb on + strand, within aldB at 3753.969 kb on - strand, within aldB at 3753.984 kb on - strand, within aldB at 3754.029 kb on + strand, within aldB at 3754.050 kb on - strand, within aldB at 3754.057 kb on - strand, within aldB at 3754.080 kb on + strand, within aldB at 3754.100 kb on - strand, within aldB at 3754.103 kb on - strand, within aldB at 3754.103 kb on - strand, within aldB at 3754.228 kb on - strand, within aldB at 3754.228 kb on - strand, within aldB at 3754.243 kb on - strand, within aldB at 3754.280 kb on + strand, within aldB at 3754.280 kb on + strand, within aldB at 3754.280 kb on + strand, within aldB at 3754.288 kb on - strand, within aldB at 3754.288 kb on - strand, within aldB at 3754.307 kb on - strand, within aldB at 3754.307 kb on - strand, within aldB at 3754.308 kb on - strand, within aldB at 3754.308 kb on - strand, within aldB at 3754.326 kb on + strand, within aldB at 3754.326 kb on + strand, within aldB at 3754.329 kb on - strand, within aldB at 3754.336 kb on - strand, within aldB at 3754.368 kb on + strand, within aldB at 3754.369 kb on - strand, within aldB at 3754.369 kb on - strand, within aldB at 3754.376 kb on - strand, within aldB at 3754.379 kb on - strand, within aldB at 3754.386 kb on - strand at 3754.386 kb on - strand at 3754.465 kb on - strand at 3754.500 kb on + strand at 3754.508 kb on - strand at 3754.639 kb on + strand at 3754.692 kb on - strand at 3754.752 kb on + strand at 3754.752 kb on + strand at 3754.895 kb on - strand, within yiaY at 3754.948 kb on + strand, within yiaY at 3754.948 kb on + strand, within yiaY at 3754.953 kb on + strand, within yiaY at 3754.953 kb on + strand, within yiaY at 3755.008 kb on + strand, within yiaY at 3755.010 kb on + strand, within yiaY at 3755.018 kb on - strand, within yiaY at 3755.037 kb on - strand, within yiaY at 3755.037 kb on - strand, within yiaY at 3755.044 kb on + strand, within yiaY at 3755.046 kb on + strand, within yiaY at 3755.066 kb on + strand, within yiaY at 3755.066 kb on + strand, within yiaY at 3755.092 kb on - strand, within yiaY at 3755.113 kb on + strand, within yiaY at 3755.115 kb on - strand, within yiaY at 3755.117 kb on - strand, within yiaY at 3755.231 kb on - strand, within yiaY at 3755.231 kb on - strand, within yiaY at 3755.231 kb on - strand, within yiaY at 3755.285 kb on - strand, within yiaY at 3755.285 kb on - strand, within yiaY at 3755.315 kb on + strand, within yiaY at 3755.319 kb on - strand, within yiaY at 3755.331 kb on - strand, within yiaY at 3755.353 kb on + strand, within yiaY at 3755.396 kb on + strand, within yiaY at 3755.396 kb on + strand, within yiaY at 3755.423 kb on + strand, within yiaY at 3755.423 kb on + strand, within yiaY at 3755.432 kb on + strand, within yiaY at 3755.435 kb on + strand, within yiaY at 3755.476 kb on - strand, within yiaY at 3755.479 kb on + strand, within yiaY at 3755.530 kb on + strand, within yiaY at 3755.535 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.545 kb on + strand, within yiaY at 3755.548 kb on + strand, within yiaY at 3755.550 kb on + strand, within yiaY at 3755.553 kb on - strand, within yiaY at 3755.553 kb on - strand, within yiaY at 3755.553 kb on - strand, within yiaY at 3755.553 kb on - strand, within yiaY at 3755.556 kb on + strand, within yiaY at 3755.556 kb on - strand, within yiaY at 3755.556 kb on - strand, within yiaY at 3755.558 kb on + strand, within yiaY at 3755.558 kb on + strand, within yiaY at 3755.562 kb on + strand, within yiaY at 3755.562 kb on + strand, within yiaY at 3755.564 kb on + strand, within yiaY at 3755.567 kb on + strand, within yiaY at 3755.567 kb on + strand, within yiaY at 3755.571 kb on + strand, within yiaY at 3755.595 kb on + strand, within yiaY at 3755.595 kb on + strand, within yiaY at 3755.617 kb on + strand, within yiaY at 3755.629 kb on - strand, within yiaY at 3755.632 kb on + strand, within yiaY at 3755.635 kb on + strand, within yiaY at 3755.636 kb on + strand, within yiaY at 3755.636 kb on + strand, within yiaY at 3755.636 kb on + strand, within yiaY at 3755.636 kb on + strand, within yiaY at 3755.636 kb on + strand, within yiaY at 3755.637 kb on + strand, within yiaY at 3755.637 kb on + strand, within yiaY at 3755.638 kb on + strand, within yiaY at 3755.638 kb on + strand, within yiaY at 3755.641 kb on - strand, within yiaY at 3755.642 kb on + strand, within yiaY at 3755.642 kb on + strand, within yiaY at 3755.653 kb on + strand, within yiaY at 3755.653 kb on + strand, within yiaY at 3755.697 kb on + strand, within yiaY at 3755.697 kb on + strand, within yiaY at 3755.702 kb on + strand, within yiaY at 3755.708 kb on + strand, within yiaY at 3755.708 kb on + strand, within yiaY at 3755.708 kb on + strand, within yiaY at 3755.713 kb on + strand, within yiaY at 3755.713 kb on + strand, within yiaY at 3755.742 kb on + strand at 3755.754 kb on + strand at 3755.754 kb on + strand at 3755.781 kb on + strand at 3755.781 kb on + strand at 3755.792 kb on + strand at 3755.792 kb on + strand at 3755.792 kb on + strand at 3755.792 kb on + strand at 3755.800 kb on - strand at 3755.812 kb on + strand at 3755.825 kb on + strand at 3755.833 kb on + strand at 3755.833 kb on + strand at 3755.833 kb on - strand at 3755.833 kb on - strand at 3755.835 kb on + strand at 3755.836 kb on + strand at 3755.836 kb on + strand at 3755.846 kb on + strand at 3755.846 kb on + strand at 3755.846 kb on + strand at 3755.846 kb on + strand at 3755.847 kb on + strand at 3755.849 kb on + strand at 3755.849 kb on + strand at 3755.857 kb on + strand at 3755.857 kb on + strand at 3755.866 kb on + strand at 3755.866 kb on + strand at 3756.092 kb on - strand at 3756.092 kb on - strand at 3756.092 kb on - strand at 3756.092 kb on - strand at 3756.103 kb on - strand at 3756.103 kb on - strand at 3756.104 kb on - strand at 3756.109 kb on - strand at 3756.109 kb on - strand at 3756.109 kb on - strand at 3756.150 kb on - strand at 3756.150 kb on - strand at 3756.150 kb on - strand at 3756.152 kb on + strand at 3756.154 kb on + strand at 3756.156 kb on - strand at 3756.156 kb on - strand at 3756.156 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.160 kb on - strand at 3756.161 kb on - strand at 3756.161 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.162 kb on - strand at 3756.184 kb on + strand at 3756.184 kb on + strand at 3756.184 kb on + strand at 3756.187 kb on - strand at 3756.187 kb on - strand at 3756.188 kb on + strand at 3756.188 kb on + strand at 3756.188 kb on + strand at 3756.188 kb on + strand at 3756.189 kb on + strand at 3756.192 kb on - strand at 3756.192 kb on - strand at 3756.192 kb on - strand at 3756.192 kb on - strand at 3756.193 kb on + strand at 3756.194 kb on + strand at 3756.194 kb on - strand at 3756.196 kb on - strand at 3756.197 kb on - strand at 3756.201 kb on + strand at 3756.204 kb on - strand at 3756.204 kb on - strand at 3756.204 kb on - strand at 3756.204 kb on - strand at 3756.204 kb on - strand at 3756.207 kb on - strand at 3756.207 kb on - strand at 3756.260 kb on - strand, within selB at 3756.268 kb on - strand, within selB at 3756.283 kb on - strand, within selB at 3756.283 kb on - strand, within selB at 3756.301 kb on + strand, within selB at 3756.301 kb on + strand, within selB at 3756.301 kb on + strand, within selB at 3756.306 kb on + strand, within selB at 3756.309 kb on - strand, within selB at 3756.309 kb on - strand, within selB at 3756.309 kb on - strand, within selB at 3756.309 kb on - strand, within selB at 3756.313 kb on - strand, within selB at 3756.314 kb on + strand, within selB at 3756.322 kb on - strand, within selB at 3756.326 kb on - strand, within selB at 3756.342 kb on + strand, within selB at 3756.352 kb on + strand, within selB at 3756.353 kb on - strand, within selB at 3756.359 kb on + strand, within selB at 3756.359 kb on + strand, within selB at 3756.364 kb on - strand, within selB at 3756.367 kb on - strand, within selB at 3756.369 kb on - strand, within selB at 3756.369 kb on - strand, within selB at 3756.370 kb on - strand, within selB at 3756.370 kb on - strand, within selB at 3756.414 kb on + strand, within selB at 3756.417 kb on - strand, within selB at 3756.429 kb on - strand, within selB at 3756.444 kb on - strand, within selB at 3756.466 kb on + strand, within selB at 3756.545 kb on - strand, within selB at 3756.658 kb on + strand, within selB at 3756.666 kb on - strand, within selB at 3756.672 kb on + strand, within selB at 3756.672 kb on + strand, within selB at 3756.672 kb on + strand, within selB at 3756.680 kb on - strand, within selB at 3756.680 kb on - strand, within selB at 3756.794 kb on + strand, within selB at 3756.802 kb on - strand, within selB at 3756.804 kb on + strand, within selB at 3756.804 kb on + strand, within selB
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) remove 3,753,730 + aldB b3588 0.48 +0.6 3,753,759 - aldB b3588 0.50 +0.1 3,753,759 - aldB b3588 0.50 +0.5 3,753,784 - aldB b3588 0.51 -0.2 3,753,857 + aldB b3588 0.56 -1.2 3,753,893 + aldB b3588 0.58 -0.4 3,753,893 - aldB b3588 0.58 -0.1 3,753,901 - aldB b3588 0.59 +0.1 3,753,937 + aldB b3588 0.61 +0.2 3,753,969 - aldB b3588 0.63 +0.3 3,753,984 - aldB b3588 0.64 +0.4 3,754,029 + aldB b3588 0.67 -0.7 3,754,050 - aldB b3588 0.68 +0.1 3,754,057 - aldB b3588 0.69 +0.4 3,754,080 + aldB b3588 0.70 +0.5 3,754,100 - aldB b3588 0.72 +0.7 3,754,103 - aldB b3588 0.72 +0.6 3,754,103 - aldB b3588 0.72 +0.1 3,754,228 - aldB b3588 0.80 +0.5 3,754,228 - aldB b3588 0.80 +0.3 3,754,243 - aldB b3588 0.81 -0.7 3,754,280 + aldB b3588 0.83 -0.1 3,754,280 + aldB b3588 0.83 +0.5 3,754,280 + aldB b3588 0.83 +0.2 3,754,288 - aldB b3588 0.84 -1.5 3,754,288 - aldB b3588 0.84 -0.5 3,754,307 - aldB b3588 0.85 -0.3 3,754,307 - aldB b3588 0.85 +0.1 3,754,308 - aldB b3588 0.85 +0.4 3,754,308 - aldB b3588 0.85 -0.3 3,754,326 + aldB b3588 0.86 -0.0 3,754,326 + aldB b3588 0.86 -0.3 3,754,329 - aldB b3588 0.87 -0.1 3,754,336 - aldB b3588 0.87 +0.3 3,754,368 + aldB b3588 0.89 -0.5 3,754,369 - aldB b3588 0.89 +0.8 3,754,369 - aldB b3588 0.89 +1.5 3,754,376 - aldB b3588 0.90 -0.2 3,754,379 - aldB b3588 0.90 +0.0 3,754,386 - -0.1 3,754,386 - +0.7 3,754,465 - -0.8 3,754,500 + -0.5 3,754,508 - -0.6 3,754,639 + +0.4 3,754,692 - +0.2 3,754,752 + -0.5 3,754,752 + -0.9 3,754,895 - yiaY b3589 0.17 +0.0 3,754,948 + yiaY b3589 0.22 +0.4 3,754,948 + yiaY b3589 0.22 +0.6 3,754,953 + yiaY b3589 0.22 +0.4 3,754,953 + yiaY b3589 0.22 -1.0 3,755,008 + yiaY b3589 0.27 -0.4 3,755,010 + yiaY b3589 0.27 -0.0 3,755,018 - yiaY b3589 0.28 +0.3 3,755,037 - yiaY b3589 0.29 -0.3 3,755,037 - yiaY b3589 0.29 +0.4 3,755,044 + yiaY b3589 0.30 -0.8 3,755,046 + yiaY b3589 0.30 +0.6 3,755,066 + yiaY b3589 0.32 +0.1 3,755,066 + yiaY b3589 0.32 +0.2 3,755,092 - yiaY b3589 0.34 -0.2 3,755,113 + yiaY b3589 0.36 -0.3 3,755,115 - yiaY b3589 0.36 +0.4 3,755,117 - yiaY b3589 0.36 +0.4 3,755,231 - yiaY b3589 0.46 -0.2 3,755,231 - yiaY b3589 0.46 -0.0 3,755,231 - yiaY b3589 0.46 +0.9 3,755,285 - yiaY b3589 0.51 +1.1 3,755,285 - yiaY b3589 0.51 +1.1 3,755,315 + yiaY b3589 0.53 -0.3 3,755,319 - yiaY b3589 0.54 -0.0 3,755,331 - yiaY b3589 0.55 -0.5 3,755,353 + yiaY b3589 0.57 +0.5 3,755,396 + yiaY b3589 0.61 +0.9 3,755,396 + yiaY b3589 0.61 +0.3 3,755,423 + yiaY b3589 0.63 -0.6 3,755,423 + yiaY b3589 0.63 -0.4 3,755,432 + yiaY b3589 0.64 -0.2 3,755,435 + yiaY b3589 0.64 +0.9 3,755,476 - yiaY b3589 0.67 -0.9 3,755,479 + yiaY b3589 0.68 -0.1 3,755,530 + yiaY b3589 0.72 -0.2 3,755,535 + yiaY b3589 0.73 -1.2 3,755,545 + yiaY b3589 0.73 +0.5 3,755,545 + yiaY b3589 0.73 -0.1 3,755,545 + yiaY b3589 0.73 +0.3 3,755,545 + yiaY b3589 0.73 +0.2 3,755,545 + yiaY b3589 0.73 -0.4 3,755,545 + yiaY b3589 0.73 -0.1 3,755,548 + yiaY b3589 0.74 -0.8 3,755,550 + yiaY b3589 0.74 -0.1 3,755,553 - yiaY b3589 0.74 +2.1 3,755,553 - yiaY b3589 0.74 +1.1 3,755,553 - yiaY b3589 0.74 -0.9 3,755,553 - yiaY b3589 0.74 -0.9 3,755,556 + yiaY b3589 0.74 -0.1 3,755,556 - yiaY b3589 0.74 +1.1 3,755,556 - yiaY b3589 0.74 +0.5 3,755,558 + yiaY b3589 0.75 -0.6 3,755,558 + yiaY b3589 0.75 -0.9 3,755,562 + yiaY b3589 0.75 +0.2 3,755,562 + yiaY b3589 0.75 -0.2 3,755,564 + yiaY b3589 0.75 -0.3 3,755,567 + yiaY b3589 0.75 +0.6 3,755,567 + yiaY b3589 0.75 +0.1 3,755,571 + yiaY b3589 0.76 -0.0 3,755,595 + yiaY b3589 0.78 -0.6 3,755,595 + yiaY b3589 0.78 -0.9 3,755,617 + yiaY b3589 0.80 -0.3 3,755,629 - yiaY b3589 0.81 +1.6 3,755,632 + yiaY b3589 0.81 -0.0 3,755,635 + yiaY b3589 0.81 +0.5 3,755,636 + yiaY b3589 0.81 -0.0 3,755,636 + yiaY b3589 0.81 +1.2 3,755,636 + yiaY b3589 0.81 +0.2 3,755,636 + yiaY b3589 0.81 +0.4 3,755,636 + yiaY b3589 0.81 +0.5 3,755,637 + yiaY b3589 0.81 +0.3 3,755,637 + yiaY b3589 0.81 -0.4 3,755,638 + yiaY b3589 0.82 +0.5 3,755,638 + yiaY b3589 0.82 +0.4 3,755,641 - yiaY b3589 0.82 +2.1 3,755,642 + yiaY b3589 0.82 +1.3 3,755,642 + yiaY b3589 0.82 +0.1 3,755,653 + yiaY b3589 0.83 -0.4 3,755,653 + yiaY b3589 0.83 -0.5 3,755,697 + yiaY b3589 0.87 -0.9 3,755,697 + yiaY b3589 0.87 -0.1 3,755,702 + yiaY b3589 0.87 -0.3 3,755,708 + yiaY b3589 0.88 -0.3 3,755,708 + yiaY b3589 0.88 -0.7 3,755,708 + yiaY b3589 0.88 +0.6 3,755,713 + yiaY b3589 0.88 -0.5 3,755,713 + yiaY b3589 0.88 +1.1 3,755,742 + -0.2 3,755,754 + -0.7 3,755,754 + -0.1 3,755,781 + -0.4 3,755,781 + +0.6 3,755,792 + +0.0 3,755,792 + -0.7 3,755,792 + -0.8 3,755,792 + +0.3 3,755,800 - +0.5 3,755,812 + -0.1 3,755,825 + +0.3 3,755,833 + -0.0 3,755,833 + +0.1 3,755,833 - +2.1 3,755,833 - +2.1 3,755,835 + -0.3 3,755,836 + +0.9 3,755,836 + -0.1 3,755,846 + -0.3 3,755,846 + -0.1 3,755,846 + -0.2 3,755,846 + -0.4 3,755,847 + +0.2 3,755,849 + +0.4 3,755,849 + +0.1 3,755,857 + +0.1 3,755,857 + -0.0 3,755,866 + +1.4 3,755,866 + +0.7 3,756,092 - -0.5 3,756,092 - -0.0 3,756,092 - +0.9 3,756,092 - -0.3 3,756,103 - +0.3 3,756,103 - -0.1 3,756,104 - -0.1 3,756,109 - +0.1 3,756,109 - -0.7 3,756,109 - +0.4 3,756,150 - +0.4 3,756,150 - +0.0 3,756,150 - +0.0 3,756,152 + +0.9 3,756,154 + -3.1 3,756,156 - -1.1 3,756,156 - +0.4 3,756,156 - -1.0 3,756,160 - +0.2 3,756,160 - -0.3 3,756,160 - -0.2 3,756,160 - -0.4 3,756,160 - +0.7 3,756,160 - +0.5 3,756,160 - -0.4 3,756,160 - -0.4 3,756,161 - +0.4 3,756,161 - +0.1 3,756,162 - +0.3 3,756,162 - -0.6 3,756,162 - +0.0 3,756,162 - -0.5 3,756,162 - -1.1 3,756,162 - -0.6 3,756,162 - +0.3 3,756,162 - -0.0 3,756,184 + -0.3 3,756,184 + +0.2 3,756,184 + -0.1 3,756,187 - -0.0 3,756,187 - -0.7 3,756,188 + +0.3 3,756,188 + -0.2 3,756,188 + +0.0 3,756,188 + +0.3 3,756,189 + +1.3 3,756,192 - +0.1 3,756,192 - +0.1 3,756,192 - -0.7 3,756,192 - -0.5 3,756,193 + -0.3 3,756,194 + -0.1 3,756,194 - +0.3 3,756,196 - -0.4 3,756,197 - +0.5 3,756,201 + -0.1 3,756,204 - -0.3 3,756,204 - +0.1 3,756,204 - -0.7 3,756,204 - +0.3 3,756,204 - +0.2 3,756,207 - -0.5 3,756,207 - +0.4 3,756,260 - selB b3590 0.12 -0.0 3,756,268 - selB b3590 0.12 +0.1 3,756,283 - selB b3590 0.13 +0.3 3,756,283 - selB b3590 0.13 -0.8 3,756,301 + selB b3590 0.14 -0.4 3,756,301 + selB b3590 0.14 +0.0 3,756,301 + selB b3590 0.14 -0.5 3,756,306 + selB b3590 0.14 +0.2 3,756,309 - selB b3590 0.15 -0.1 3,756,309 - selB b3590 0.15 -0.5 3,756,309 - selB b3590 0.15 -0.5 3,756,309 - selB b3590 0.15 -0.4 3,756,313 - selB b3590 0.15 +0.1 3,756,314 + selB b3590 0.15 +0.5 3,756,322 - selB b3590 0.15 -0.5 3,756,326 - selB b3590 0.16 +0.0 3,756,342 + selB b3590 0.16 -0.1 3,756,352 + selB b3590 0.17 -0.5 3,756,353 - selB b3590 0.17 -0.5 3,756,359 + selB b3590 0.17 +0.4 3,756,359 + selB b3590 0.17 -0.1 3,756,364 - selB b3590 0.18 -0.3 3,756,367 - selB b3590 0.18 +0.1 3,756,369 - selB b3590 0.18 -0.6 3,756,369 - selB b3590 0.18 +0.0 3,756,370 - selB b3590 0.18 +0.3 3,756,370 - selB b3590 0.18 +1.0 3,756,414 + selB b3590 0.20 +0.4 3,756,417 - selB b3590 0.20 +0.2 3,756,429 - selB b3590 0.21 -0.3 3,756,444 - selB b3590 0.22 +0.6 3,756,466 + selB b3590 0.23 -0.6 3,756,545 - selB b3590 0.27 -0.2 3,756,658 + selB b3590 0.33 -0.3 3,756,666 - selB b3590 0.34 +0.0 3,756,672 + selB b3590 0.34 -0.1 3,756,672 + selB b3590 0.34 -0.3 3,756,672 + selB b3590 0.34 +0.1 3,756,680 - selB b3590 0.35 -1.3 3,756,680 - selB b3590 0.35 -1.4 3,756,794 + selB b3590 0.41 +0.3 3,756,802 - selB b3590 0.41 -0.6 3,756,804 + selB b3590 0.41 -0.1 3,756,804 + selB b3590 0.41 -0.2
Or see this region's nucleotide sequence