Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfiQ and pssA are separated by 113 nucleotides pssA and yfiM are separated by 45 nucleotides yfiM and kgtP overlap by 4 nucleotides
b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635
yfiQ
b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104
pssA
b2586: yfiM - predicted protein (RefSeq), at 2,722,150 to 2,722,473
yfiM
b2587: kgtP - alpha-ketoglutarate transporter (NCBI), at 2,722,470 to 2,723,768
kgtP
Position (kb)
2720
2721
2722
2723 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2719.852 kb on - strand, within yfiQ at 2719.931 kb on - strand, within yfiQ at 2720.013 kb on + strand, within yfiQ at 2720.013 kb on + strand, within yfiQ at 2720.093 kb on - strand, within yfiQ at 2720.105 kb on - strand, within yfiQ at 2720.235 kb on - strand, within yfiQ at 2720.288 kb on - strand, within yfiQ at 2720.288 kb on - strand, within yfiQ at 2720.368 kb on + strand, within yfiQ at 2720.434 kb on - strand at 2720.438 kb on + strand at 2720.440 kb on - strand at 2720.440 kb on - strand at 2720.545 kb on + strand at 2720.545 kb on + strand at 2720.642 kb on - strand at 2721.348 kb on + strand, within pssA at 2722.110 kb on + strand at 2722.113 kb on - strand at 2722.115 kb on + strand at 2722.171 kb on + strand at 2722.227 kb on + strand, within yfiM at 2722.332 kb on + strand, within yfiM at 2722.387 kb on - strand, within yfiM at 2722.415 kb on + strand, within yfiM at 2722.435 kb on + strand, within yfiM at 2722.539 kb on - strand at 2722.539 kb on - strand at 2722.557 kb on + strand at 2722.557 kb on + strand at 2722.570 kb on + strand at 2722.604 kb on - strand, within kgtP at 2722.604 kb on - strand, within kgtP at 2722.633 kb on + strand, within kgtP at 2722.641 kb on - strand, within kgtP at 2722.783 kb on + strand, within kgtP at 2722.801 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2723.060 kb on - strand, within kgtP at 2723.060 kb on - strand, within kgtP
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) remove 2,719,852 - yfiQ b2584 0.71 +0.4 2,719,931 - yfiQ b2584 0.74 -0.4 2,720,013 + yfiQ b2584 0.77 -0.7 2,720,013 + yfiQ b2584 0.77 -0.2 2,720,093 - yfiQ b2584 0.80 -1.1 2,720,105 - yfiQ b2584 0.80 -0.3 2,720,235 - yfiQ b2584 0.85 -0.2 2,720,288 - yfiQ b2584 0.87 -0.1 2,720,288 - yfiQ b2584 0.87 +0.7 2,720,368 + yfiQ b2584 0.90 -0.4 2,720,434 - -2.4 2,720,438 + -0.4 2,720,440 - +0.2 2,720,440 - +0.4 2,720,545 + +0.4 2,720,545 + +1.0 2,720,642 - +0.3 2,721,348 + pssA b2585 0.44 -0.6 2,722,110 + -0.2 2,722,113 - -0.5 2,722,115 + -0.0 2,722,171 + -0.0 2,722,227 + yfiM b2586 0.24 -0.4 2,722,332 + yfiM b2586 0.56 -0.1 2,722,387 - yfiM b2586 0.73 -0.9 2,722,415 + yfiM b2586 0.82 -0.2 2,722,435 + yfiM b2586 0.88 +1.7 2,722,539 - +0.7 2,722,539 - +0.8 2,722,557 + +0.8 2,722,557 + -0.6 2,722,570 + +1.7 2,722,604 - kgtP b2587 0.10 -0.1 2,722,604 - kgtP b2587 0.10 +0.8 2,722,633 + kgtP b2587 0.13 +0.6 2,722,641 - kgtP b2587 0.13 -0.2 2,722,783 + kgtP b2587 0.24 +0.3 2,722,801 + kgtP b2587 0.25 +0.0 2,722,943 + kgtP b2587 0.36 +2.3 2,722,943 + kgtP b2587 0.36 -0.3 2,723,060 - kgtP b2587 0.45 +0.1 2,723,060 - kgtP b2587 0.45 +0.8
Or see this region's nucleotide sequence