Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbD and b1408 are separated by 170 nucleotides b1408 and ynbB overlap by 1 nucleotides ynbB and ynbC are separated by 15 nucleotides
b1407: ydbD - orf, hypothetical protein (VIMSS), at 1,473,168 to 1,475,474
ydbD
b1408: b1408 - probable enzyme (VIMSS), at 1,475,645 to 1,476,250
b1408
b1409: ynbB - predicted CDP-diglyceride synthase (NCBI), at 1,476,250 to 1,477,146
ynbB
b1410: ynbC - predicted hydrolase (NCBI), at 1,477,162 to 1,478,919
ynbC
Position (kb)
1475
1476
1477 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1474.854 kb on + strand, within ydbD at 1474.889 kb on + strand, within ydbD at 1474.897 kb on - strand, within ydbD at 1474.943 kb on - strand, within ydbD at 1475.091 kb on - strand, within ydbD at 1475.101 kb on + strand, within ydbD at 1475.101 kb on - strand, within ydbD at 1475.134 kb on - strand, within ydbD at 1475.145 kb on + strand, within ydbD at 1475.145 kb on + strand, within ydbD at 1475.145 kb on - strand, within ydbD at 1475.145 kb on - strand, within ydbD at 1475.155 kb on - strand, within ydbD at 1475.183 kb on + strand, within ydbD at 1475.232 kb on + strand, within ydbD at 1475.242 kb on + strand, within ydbD at 1475.254 kb on + strand at 1475.348 kb on + strand at 1475.383 kb on - strand at 1475.610 kb on - strand at 1475.691 kb on - strand at 1475.719 kb on + strand, within b1408 at 1475.771 kb on + strand, within b1408 at 1475.771 kb on + strand, within b1408 at 1475.790 kb on + strand, within b1408 at 1475.838 kb on + strand, within b1408 at 1475.838 kb on + strand, within b1408 at 1475.850 kb on + strand, within b1408 at 1475.850 kb on + strand, within b1408 at 1475.862 kb on + strand, within b1408 at 1475.862 kb on + strand, within b1408 at 1475.866 kb on + strand, within b1408 at 1475.866 kb on + strand, within b1408 at 1475.866 kb on - strand, within b1408 at 1475.903 kb on + strand, within b1408 at 1476.147 kb on + strand, within b1408 at 1476.147 kb on + strand, within b1408 at 1476.882 kb on - strand, within ynbB at 1476.882 kb on - strand, within ynbB at 1476.894 kb on - strand, within ynbB at 1476.902 kb on + strand, within ynbB at 1476.924 kb on + strand, within ynbB at 1476.934 kb on + strand, within ynbB at 1476.934 kb on + strand, within ynbB at 1477.000 kb on + strand, within ynbB at 1477.008 kb on + strand, within ynbB at 1477.055 kb on + strand, within ynbB at 1477.064 kb on - strand at 1477.066 kb on + strand at 1477.074 kb on + strand at 1477.088 kb on + strand at 1477.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) remove 1,474,854 + ydbD b1407 0.73 +1.8 1,474,889 + ydbD b1407 0.75 +1.8 1,474,897 - ydbD b1407 0.75 +1.8 1,474,943 - ydbD b1407 0.77 +0.8 1,475,091 - ydbD b1407 0.83 +0.2 1,475,101 + ydbD b1407 0.84 +1.8 1,475,101 - ydbD b1407 0.84 -0.5 1,475,134 - ydbD b1407 0.85 +1.0 1,475,145 + ydbD b1407 0.86 +0.8 1,475,145 + ydbD b1407 0.86 -1.9 1,475,145 - ydbD b1407 0.86 +1.1 1,475,145 - ydbD b1407 0.86 -0.4 1,475,155 - ydbD b1407 0.86 -1.2 1,475,183 + ydbD b1407 0.87 +1.8 1,475,232 + ydbD b1407 0.89 -1.0 1,475,242 + ydbD b1407 0.90 -0.0 1,475,254 + -0.3 1,475,348 + +0.4 1,475,383 - -0.6 1,475,610 - +0.4 1,475,691 - +1.8 1,475,719 + b1408 b1408 0.12 -0.5 1,475,771 + b1408 b1408 0.21 +0.9 1,475,771 + b1408 b1408 0.21 +0.8 1,475,790 + b1408 b1408 0.24 +0.7 1,475,838 + b1408 b1408 0.32 -0.1 1,475,838 + b1408 b1408 0.32 +0.9 1,475,850 + b1408 b1408 0.34 +2.2 1,475,850 + b1408 b1408 0.34 +0.1 1,475,862 + b1408 b1408 0.36 -0.1 1,475,862 + b1408 b1408 0.36 +0.2 1,475,866 + b1408 b1408 0.36 -0.4 1,475,866 + b1408 b1408 0.36 -0.4 1,475,866 - b1408 b1408 0.36 +1.8 1,475,903 + b1408 b1408 0.43 -1.4 1,476,147 + b1408 b1408 0.83 +0.2 1,476,147 + b1408 b1408 0.83 +0.5 1,476,882 - ynbB b1409 0.70 +0.8 1,476,882 - ynbB b1409 0.70 +1.8 1,476,894 - ynbB b1409 0.72 +1.8 1,476,902 + ynbB b1409 0.73 -0.8 1,476,924 + ynbB b1409 0.75 -0.5 1,476,934 + ynbB b1409 0.76 -0.8 1,476,934 + ynbB b1409 0.76 +1.8 1,477,000 + ynbB b1409 0.84 +1.8 1,477,008 + ynbB b1409 0.85 +0.5 1,477,055 + ynbB b1409 0.90 -0.2 1,477,064 - +1.8 1,477,066 + +1.8 1,477,074 + +1.4 1,477,088 + -1.0 1,477,214 - -1.4
Or see this region's nucleotide sequence