Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acnB and yacL are separated by 175 nucleotides yacL and speD are separated by 37 nucleotides speD and speE are separated by 15 nucleotides
b0118: acnB - aconitate hydratase (NCBI), at 131,615 to 134,212
acnB
b0119: yacL - orf, hypothetical protein (VIMSS), at 134,388 to 134,750
yacL
b0120: speD - S-adenosylmethionine decarboxylase proenzyme (NCBI), at 134,788 to 135,582
speD
b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464
speE
Position (kb) 134
135 Strain fitness (log2 ratio) -1
0
1 at 134.194 kb on + strand at 134.338 kb on + strand at 134.338 kb on + strand at 134.373 kb on + strand at 134.384 kb on + strand at 134.410 kb on + strand at 134.410 kb on + strand at 134.418 kb on - strand at 134.429 kb on + strand, within yacL at 134.430 kb on - strand, within yacL at 134.430 kb on - strand, within yacL at 134.432 kb on + strand, within yacL at 134.445 kb on + strand, within yacL at 134.445 kb on + strand, within yacL at 134.454 kb on - strand, within yacL at 134.454 kb on - strand, within yacL at 134.454 kb on - strand, within yacL at 134.456 kb on + strand, within yacL at 134.456 kb on + strand, within yacL at 134.549 kb on + strand, within yacL at 134.549 kb on + strand, within yacL at 134.553 kb on + strand, within yacL at 134.580 kb on + strand, within yacL at 134.598 kb on - strand, within yacL at 134.601 kb on - strand, within yacL at 134.657 kb on + strand, within yacL at 134.657 kb on + strand, within yacL at 134.666 kb on - strand, within yacL at 134.683 kb on + strand, within yacL at 134.690 kb on - strand, within yacL at 134.690 kb on - strand, within yacL at 134.831 kb on + strand at 134.878 kb on - strand, within speD at 134.878 kb on - strand, within speD at 134.899 kb on - strand, within speD at 134.899 kb on - strand, within speD at 134.900 kb on + strand, within speD at 134.900 kb on + strand, within speD at 134.903 kb on - strand, within speD at 134.980 kb on + strand, within speD at 134.980 kb on + strand, within speD at 135.008 kb on + strand, within speD at 135.018 kb on + strand, within speD at 135.048 kb on + strand, within speD at 135.048 kb on + strand, within speD at 135.237 kb on - strand, within speD at 135.238 kb on - strand, within speD at 135.270 kb on - strand, within speD at 135.351 kb on + strand, within speD at 135.355 kb on - strand, within speD at 135.355 kb on - strand, within speD at 135.359 kb on - strand, within speD at 135.359 kb on - strand, within speD at 135.368 kb on + strand, within speD at 135.431 kb on + strand, within speD at 135.444 kb on + strand, within speD at 135.457 kb on - strand, within speD at 135.482 kb on - strand, within speD at 135.482 kb on - strand, within speD at 135.643 kb on + strand at 135.705 kb on - strand, within speE at 135.749 kb on - strand, within speE
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 mM (C) remove 134,194 + +0.0 134,338 + -0.3 134,338 + -0.5 134,373 + +0.6 134,384 + +0.6 134,410 + +0.2 134,410 + +0.2 134,418 - +0.2 134,429 + yacL b0119 0.11 -0.3 134,430 - yacL b0119 0.12 +0.1 134,430 - yacL b0119 0.12 -0.3 134,432 + yacL b0119 0.12 -0.3 134,445 + yacL b0119 0.16 +0.1 134,445 + yacL b0119 0.16 -0.1 134,454 - yacL b0119 0.18 -0.1 134,454 - yacL b0119 0.18 -0.3 134,454 - yacL b0119 0.18 +0.2 134,456 + yacL b0119 0.19 -0.1 134,456 + yacL b0119 0.19 -0.2 134,549 + yacL b0119 0.44 +0.1 134,549 + yacL b0119 0.44 -0.3 134,553 + yacL b0119 0.45 -0.2 134,580 + yacL b0119 0.53 -0.4 134,598 - yacL b0119 0.58 -0.1 134,601 - yacL b0119 0.59 -0.1 134,657 + yacL b0119 0.74 -0.1 134,657 + yacL b0119 0.74 +0.2 134,666 - yacL b0119 0.77 +0.3 134,683 + yacL b0119 0.81 -0.8 134,690 - yacL b0119 0.83 -0.2 134,690 - yacL b0119 0.83 +1.2 134,831 + +0.1 134,878 - speD b0120 0.11 +0.1 134,878 - speD b0120 0.11 +0.1 134,899 - speD b0120 0.14 -0.7 134,899 - speD b0120 0.14 -0.0 134,900 + speD b0120 0.14 -0.1 134,900 + speD b0120 0.14 +0.6 134,903 - speD b0120 0.14 +0.2 134,980 + speD b0120 0.24 -0.0 134,980 + speD b0120 0.24 +0.5 135,008 + speD b0120 0.28 +0.3 135,018 + speD b0120 0.29 -0.1 135,048 + speD b0120 0.33 +0.6 135,048 + speD b0120 0.33 -1.4 135,237 - speD b0120 0.56 +0.1 135,238 - speD b0120 0.57 +0.4 135,270 - speD b0120 0.61 +0.3 135,351 + speD b0120 0.71 -0.1 135,355 - speD b0120 0.71 +0.0 135,355 - speD b0120 0.71 +0.9 135,359 - speD b0120 0.72 +0.7 135,359 - speD b0120 0.72 +1.2 135,368 + speD b0120 0.73 +0.5 135,431 + speD b0120 0.81 -0.5 135,444 + speD b0120 0.83 -0.1 135,457 - speD b0120 0.84 +0.4 135,482 - speD b0120 0.87 -0.1 135,482 - speD b0120 0.87 +0.4 135,643 + +0.1 135,705 - speE b0121 0.12 -0.9 135,749 - speE b0121 0.17 +0.3
Or see this region's nucleotide sequence