Strain Fitness in Escherichia coli BW25113 around b3248

Experiment: Uridine 0.4095 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcafA and yhdE overlap by 11 nucleotidesyhdE and mreD are separated by 8 nucleotidesmreD and mreC overlap by 1 nucleotides b3247: cafA - bundles of cytoplasmic filaments (VIMSS), at 3,394,348 to 3,395,817 cafA b3248: yhdE - Maf-like protein (NCBI), at 3,395,807 to 3,396,400 yhdE b3249: mreD - cell wall structural complex MreBCD transmembrane component MreD (NCBI), at 3,396,409 to 3,396,897 mreD b3250: mreC - cell wall structural complex MreBCD transmembrane component MreC (NCBI), at 3,396,897 to 3,398,000 mreC Position (kb) 3395 3396 3397Strain fitness (log2 ratio) -2 -1 0 1at 3394.818 kb on + strand, within cafAat 3394.898 kb on + strand, within cafAat 3394.922 kb on + strand, within cafAat 3395.036 kb on + strand, within cafAat 3395.041 kb on - strand, within cafAat 3395.041 kb on - strand, within cafAat 3395.044 kb on - strand, within cafAat 3395.097 kb on - strand, within cafAat 3395.105 kb on - strand, within cafAat 3395.121 kb on + strand, within cafAat 3395.139 kb on - strand, within cafAat 3395.188 kb on + strand, within cafAat 3395.193 kb on + strand, within cafAat 3395.201 kb on - strand, within cafAat 3395.231 kb on + strand, within cafAat 3395.254 kb on - strand, within cafAat 3395.254 kb on - strand, within cafAat 3395.272 kb on - strand, within cafAat 3395.283 kb on + strand, within cafAat 3395.291 kb on - strand, within cafAat 3395.292 kb on + strand, within cafAat 3395.292 kb on + strand, within cafAat 3395.318 kb on - strand, within cafAat 3395.318 kb on - strand, within cafAat 3395.328 kb on - strand, within cafAat 3395.330 kb on + strand, within cafAat 3395.330 kb on + strand, within cafAat 3395.335 kb on + strand, within cafAat 3395.387 kb on - strand, within cafAat 3395.397 kb on + strand, within cafAat 3395.397 kb on + strand, within cafAat 3395.414 kb on - strand, within cafAat 3395.479 kb on - strand, within cafAat 3395.532 kb on + strand, within cafAat 3395.532 kb on + strand, within cafAat 3395.542 kb on + strand, within cafAat 3395.560 kb on - strand, within cafAat 3395.568 kb on - strand, within cafAat 3395.568 kb on - strand, within cafAat 3395.599 kb on + strand, within cafAat 3395.599 kb on + strand, within cafAat 3395.666 kb on - strand, within cafAat 3395.677 kb on + strandat 3395.689 kb on + strandat 3395.690 kb on + strandat 3395.690 kb on + strandat 3395.787 kb on + strandat 3395.893 kb on - strand, within yhdEat 3395.897 kb on - strand, within yhdEat 3395.897 kb on - strand, within yhdEat 3395.923 kb on + strand, within yhdEat 3395.923 kb on + strand, within yhdEat 3395.941 kb on + strand, within yhdEat 3395.956 kb on - strand, within yhdEat 3395.964 kb on - strand, within yhdEat 3395.966 kb on + strand, within yhdEat 3395.987 kb on - strand, within yhdEat 3395.987 kb on - strand, within yhdEat 3396.058 kb on - strand, within yhdEat 3396.117 kb on - strand, within yhdEat 3396.139 kb on + strand, within yhdEat 3396.166 kb on - strand, within yhdEat 3396.166 kb on - strand, within yhdEat 3396.176 kb on - strand, within yhdEat 3396.186 kb on - strand, within yhdEat 3396.192 kb on + strand, within yhdEat 3396.231 kb on + strand, within yhdEat 3396.231 kb on + strand, within yhdEat 3396.256 kb on + strand, within yhdEat 3396.267 kb on + strand, within yhdEat 3396.269 kb on + strand, within yhdEat 3396.275 kb on - strand, within yhdEat 3396.275 kb on - strand, within yhdEat 3396.287 kb on - strand, within yhdEat 3396.287 kb on - strand, within yhdEat 3396.307 kb on - strand, within yhdEat 3396.317 kb on - strand, within yhdEat 3396.333 kb on - strand, within yhdEat 3396.344 kb on - strandat 3396.344 kb on - strandat 3396.364 kb on - strandat 3396.364 kb on - strandat 3396.417 kb on - strandat 3396.924 kb on - strandat 3396.924 kb on - strandat 3396.934 kb on - strandat 3396.962 kb on - strandat 3397.084 kb on - strand, within mreCat 3397.174 kb on - strand, within mreC

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C)
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3,394,818 + cafA b3247 0.32 +0.7
3,394,898 + cafA b3247 0.37 -0.1
3,394,922 + cafA b3247 0.39 -0.1
3,395,036 + cafA b3247 0.47 -0.5
3,395,041 - cafA b3247 0.47 +0.6
3,395,041 - cafA b3247 0.47 -1.0
3,395,044 - cafA b3247 0.47 +0.6
3,395,097 - cafA b3247 0.51 -0.1
3,395,105 - cafA b3247 0.51 -0.6
3,395,121 + cafA b3247 0.53 +0.1
3,395,139 - cafA b3247 0.54 +0.2
3,395,188 + cafA b3247 0.57 -0.1
3,395,193 + cafA b3247 0.57 -0.8
3,395,201 - cafA b3247 0.58 -0.1
3,395,231 + cafA b3247 0.60 -0.9
3,395,254 - cafA b3247 0.62 +0.7
3,395,254 - cafA b3247 0.62 +0.2
3,395,272 - cafA b3247 0.63 -0.8
3,395,283 + cafA b3247 0.64 -0.2
3,395,291 - cafA b3247 0.64 +0.8
3,395,292 + cafA b3247 0.64 +0.2
3,395,292 + cafA b3247 0.64 +1.4
3,395,318 - cafA b3247 0.66 +0.8
3,395,318 - cafA b3247 0.66 +0.1
3,395,328 - cafA b3247 0.67 +0.8
3,395,330 + cafA b3247 0.67 +0.1
3,395,330 + cafA b3247 0.67 -0.5
3,395,335 + cafA b3247 0.67 +0.1
3,395,387 - cafA b3247 0.71 +0.5
3,395,397 + cafA b3247 0.71 +0.3
3,395,397 + cafA b3247 0.71 -0.4
3,395,414 - cafA b3247 0.73 -1.0
3,395,479 - cafA b3247 0.77 +0.1
3,395,532 + cafA b3247 0.81 +0.2
3,395,532 + cafA b3247 0.81 +1.6
3,395,542 + cafA b3247 0.81 +0.0
3,395,560 - cafA b3247 0.82 -2.0
3,395,568 - cafA b3247 0.83 -0.2
3,395,568 - cafA b3247 0.83 -0.0
3,395,599 + cafA b3247 0.85 -0.8
3,395,599 + cafA b3247 0.85 +0.2
3,395,666 - cafA b3247 0.90 -1.1
3,395,677 + -1.7
3,395,689 + -0.6
3,395,690 + +0.5
3,395,690 + +1.4
3,395,787 + +0.4
3,395,893 - yhdE b3248 0.14 +0.2
3,395,897 - yhdE b3248 0.15 +0.6
3,395,897 - yhdE b3248 0.15 -0.1
3,395,923 + yhdE b3248 0.20 +0.1
3,395,923 + yhdE b3248 0.20 +0.5
3,395,941 + yhdE b3248 0.23 +0.3
3,395,956 - yhdE b3248 0.25 +0.2
3,395,964 - yhdE b3248 0.26 -0.5
3,395,966 + yhdE b3248 0.27 -0.2
3,395,987 - yhdE b3248 0.30 +0.6
3,395,987 - yhdE b3248 0.30 +0.1
3,396,058 - yhdE b3248 0.42 +0.3
3,396,117 - yhdE b3248 0.52 +1.0
3,396,139 + yhdE b3248 0.56 -0.2
3,396,166 - yhdE b3248 0.60 -1.1
3,396,166 - yhdE b3248 0.60 +0.4
3,396,176 - yhdE b3248 0.62 -1.4
3,396,186 - yhdE b3248 0.64 -0.6
3,396,192 + yhdE b3248 0.65 -0.3
3,396,231 + yhdE b3248 0.71 -0.0
3,396,231 + yhdE b3248 0.71 +0.5
3,396,256 + yhdE b3248 0.76 -0.0
3,396,267 + yhdE b3248 0.77 -0.5
3,396,269 + yhdE b3248 0.78 +1.1
3,396,275 - yhdE b3248 0.79 +0.2
3,396,275 - yhdE b3248 0.79 -0.5
3,396,287 - yhdE b3248 0.81 -0.9
3,396,287 - yhdE b3248 0.81 -0.6
3,396,307 - yhdE b3248 0.84 -0.0
3,396,317 - yhdE b3248 0.86 +0.3
3,396,333 - yhdE b3248 0.89 +0.4
3,396,344 - +0.5
3,396,344 - +1.3
3,396,364 - +0.3
3,396,364 - -1.3
3,396,417 - +0.0
3,396,924 - -0.4
3,396,924 - -1.5
3,396,934 - -2.4
3,396,962 - -1.3
3,397,084 - mreC b3250 0.17 +0.3
3,397,174 - mreC b3250 0.25 -0.9

Or see this region's nucleotide sequence