Experiment: Uridine 0.4095 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speC and yqgA are separated by 397 nucleotides yqgA and pheV are separated by 105 nucleotides pheV and yghD are separated by 148 nucleotides yghD and yghE are separated by 1 nucleotides
b2965: speC - ornithine decarboxylase isozyme (VIMSS), at 3,105,042 to 3,107,177
speC
b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282
yqgA
b2967: pheV - tRNA-Phe (NCBI), at 3,108,388 to 3,108,463
pheV
b2968: yghD - predicted secretion pathway M-type protein, membrane anchored (NCBI), at 3,108,612 to 3,109,148
yghD
b2969: yghE - predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq), at 3,109,150 to 3,110,112
yghE
Position (kb)
3107
3108
3109 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3106.727 kb on - strand, within speC at 3106.732 kb on - strand, within speC at 3106.811 kb on + strand, within speC at 3106.811 kb on - strand, within speC at 3106.825 kb on + strand, within speC at 3106.825 kb on + strand, within speC at 3106.841 kb on + strand, within speC at 3106.856 kb on - strand, within speC at 3106.856 kb on - strand, within speC at 3107.101 kb on + strand at 3107.101 kb on + strand at 3107.147 kb on - strand at 3107.246 kb on + strand at 3107.246 kb on + strand at 3107.401 kb on + strand at 3107.410 kb on - strand at 3107.496 kb on - strand at 3107.496 kb on - strand at 3107.522 kb on + strand at 3107.580 kb on - strand at 3107.608 kb on + strand at 3107.611 kb on + strand at 3107.611 kb on + strand at 3107.616 kb on - strand at 3107.895 kb on + strand, within yqgA at 3107.918 kb on - strand, within yqgA at 3107.932 kb on - strand, within yqgA at 3108.033 kb on + strand, within yqgA at 3108.117 kb on + strand, within yqgA at 3108.160 kb on + strand, within yqgA at 3108.181 kb on - strand, within yqgA at 3108.221 kb on - strand at 3108.222 kb on + strand at 3108.222 kb on + strand at 3108.378 kb on - strand at 3108.378 kb on - strand at 3108.593 kb on + strand at 3108.629 kb on - strand at 3108.629 kb on - strand at 3108.649 kb on + strand at 3108.766 kb on + strand, within yghD at 3108.821 kb on + strand, within yghD at 3108.833 kb on - strand, within yghD at 3108.833 kb on - strand, within yghD at 3108.878 kb on + strand, within yghD at 3108.931 kb on - strand, within yghD at 3108.940 kb on + strand, within yghD at 3108.940 kb on + strand, within yghD at 3109.078 kb on - strand, within yghD at 3109.098 kb on - strand at 3109.123 kb on - strand at 3109.174 kb on + strand at 3109.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C) remove 3,106,727 - speC b2965 0.79 +0.8 3,106,732 - speC b2965 0.79 +0.6 3,106,811 + speC b2965 0.83 +0.9 3,106,811 - speC b2965 0.83 +0.7 3,106,825 + speC b2965 0.83 +0.3 3,106,825 + speC b2965 0.83 +0.7 3,106,841 + speC b2965 0.84 +0.8 3,106,856 - speC b2965 0.85 +0.5 3,106,856 - speC b2965 0.85 +0.7 3,107,101 + +1.2 3,107,101 + +1.1 3,107,147 - +0.8 3,107,246 + +0.3 3,107,246 + +0.6 3,107,401 + +0.3 3,107,410 - -0.8 3,107,496 - +0.5 3,107,496 - -0.4 3,107,522 + +0.4 3,107,580 - -0.0 3,107,608 + -0.9 3,107,611 + +0.3 3,107,611 + -1.1 3,107,616 - -0.2 3,107,895 + yqgA b2966 0.45 +0.1 3,107,918 - yqgA b2966 0.48 -0.1 3,107,932 - yqgA b2966 0.50 +0.1 3,108,033 + yqgA b2966 0.65 +0.7 3,108,117 + yqgA b2966 0.77 +0.5 3,108,160 + yqgA b2966 0.83 +0.0 3,108,181 - yqgA b2966 0.86 -0.1 3,108,221 - -0.4 3,108,222 + +0.2 3,108,222 + +1.1 3,108,378 - +0.8 3,108,378 - -0.7 3,108,593 + +0.3 3,108,629 - -1.2 3,108,629 - +1.0 3,108,649 + -0.9 3,108,766 + yghD b2968 0.29 -0.1 3,108,821 + yghD b2968 0.39 +0.5 3,108,833 - yghD b2968 0.41 -3.0 3,108,833 - yghD b2968 0.41 -0.7 3,108,878 + yghD b2968 0.50 -0.1 3,108,931 - yghD b2968 0.59 -0.0 3,108,940 + yghD b2968 0.61 +0.7 3,108,940 + yghD b2968 0.61 +0.4 3,109,078 - yghD b2968 0.87 +0.4 3,109,098 - -0.4 3,109,123 - -1.3 3,109,174 + +0.1 3,109,220 - -0.6
Or see this region's nucleotide sequence