Strain Fitness in Escherichia coli BW25113 around b0967

Experiment: Uridine 0.4095 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb0965 and hspQ are separated by 44 nucleotideshspQ and yccW are separated by 57 nucleotidesyccW and yccX are separated by 94 nucleotidesyccX and yccK overlap by 4 nucleotidesyccK and yccA are separated by 90 nucleotides b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965 b0966: hspQ - DNA-binding protein, hemimethylated (RefSeq), at 1,027,627 to 1,027,944 hspQ b0967: yccW - predicted methyltransferase (RefSeq), at 1,028,002 to 1,029,192 yccW b0968: yccX - predicted acylphosphatase (NCBI), at 1,029,287 to 1,029,565 yccX b0969: yccK - putative sulfite reductase (EC 1.8.-.-) (VIMSS), at 1,029,562 to 1,029,891 yccK b0970: yccA - inner membrane protein (NCBI), at 1,029,982 to 1,030,641 yccA Position (kb) 1028 1029 1030Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1027.060 kb on + strandat 1027.060 kb on + strandat 1027.085 kb on + strandat 1027.145 kb on + strandat 1027.153 kb on - strandat 1027.293 kb on - strand, within b0965at 1027.466 kb on + strand, within b0965at 1027.570 kb on - strandat 1027.626 kb on - strandat 1027.633 kb on - strandat 1027.658 kb on - strandat 1027.667 kb on + strand, within hspQat 1027.674 kb on - strand, within hspQat 1027.675 kb on - strand, within hspQat 1027.695 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.755 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.885 kb on + strand, within hspQat 1028.057 kb on - strandat 1028.120 kb on + strandat 1028.328 kb on + strand, within yccWat 1028.328 kb on + strand, within yccWat 1028.373 kb on - strand, within yccWat 1028.378 kb on - strand, within yccWat 1028.378 kb on - strand, within yccWat 1028.425 kb on - strand, within yccWat 1028.478 kb on + strand, within yccWat 1028.509 kb on - strand, within yccWat 1028.579 kb on - strand, within yccWat 1028.579 kb on - strand, within yccWat 1028.619 kb on + strand, within yccWat 1028.685 kb on - strand, within yccWat 1028.796 kb on + strand, within yccWat 1028.796 kb on + strand, within yccWat 1028.841 kb on + strand, within yccWat 1028.846 kb on + strand, within yccWat 1028.901 kb on - strand, within yccWat 1028.975 kb on + strand, within yccWat 1028.984 kb on - strand, within yccWat 1029.155 kb on - strandat 1029.171 kb on - strandat 1029.269 kb on + strandat 1029.273 kb on - strandat 1029.273 kb on - strandat 1029.279 kb on - strandat 1029.322 kb on - strand, within yccXat 1029.322 kb on - strand, within yccXat 1029.343 kb on + strand, within yccXat 1029.348 kb on + strand, within yccXat 1029.477 kb on - strand, within yccXat 1029.477 kb on - strand, within yccXat 1029.501 kb on + strand, within yccXat 1029.529 kb on - strand, within yccXat 1029.529 kb on - strand, within yccXat 1029.860 kb on + strandat 1029.960 kb on + strandat 1029.980 kb on + strandat 1029.980 kb on + strandat 1030.084 kb on + strand, within yccAat 1030.154 kb on + strand, within yccA

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C)
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1,027,060 + -1.9
1,027,060 + -1.2
1,027,085 + -1.0
1,027,145 + -1.0
1,027,153 - +0.7
1,027,293 - b0965 b0965 0.30 +0.3
1,027,466 + b0965 b0965 0.72 +0.5
1,027,570 - -0.9
1,027,626 - -0.1
1,027,633 - -0.6
1,027,658 - +0.0
1,027,667 + hspQ b0966 0.13 -0.7
1,027,674 - hspQ b0966 0.15 +0.1
1,027,675 - hspQ b0966 0.15 +0.7
1,027,695 + hspQ b0966 0.21 -0.1
1,027,747 + hspQ b0966 0.38 +1.7
1,027,747 + hspQ b0966 0.38 +0.6
1,027,755 - hspQ b0966 0.40 -0.4
1,027,810 - hspQ b0966 0.58 +0.6
1,027,810 - hspQ b0966 0.58 -0.0
1,027,865 - hspQ b0966 0.75 +0.3
1,027,865 - hspQ b0966 0.75 +0.3
1,027,885 + hspQ b0966 0.81 +1.2
1,028,057 - -0.1
1,028,120 + -1.0
1,028,328 + yccW b0967 0.27 +0.9
1,028,328 + yccW b0967 0.27 +0.2
1,028,373 - yccW b0967 0.31 +0.6
1,028,378 - yccW b0967 0.32 -0.4
1,028,378 - yccW b0967 0.32 -0.2
1,028,425 - yccW b0967 0.36 -1.6
1,028,478 + yccW b0967 0.40 +0.0
1,028,509 - yccW b0967 0.43 -1.7
1,028,579 - yccW b0967 0.48 +0.7
1,028,579 - yccW b0967 0.48 -0.4
1,028,619 + yccW b0967 0.52 -0.1
1,028,685 - yccW b0967 0.57 -0.2
1,028,796 + yccW b0967 0.67 +0.9
1,028,796 + yccW b0967 0.67 -2.9
1,028,841 + yccW b0967 0.70 +0.6
1,028,846 + yccW b0967 0.71 +0.6
1,028,901 - yccW b0967 0.75 +0.3
1,028,975 + yccW b0967 0.82 +0.6
1,028,984 - yccW b0967 0.82 -1.1
1,029,155 - -0.5
1,029,171 - -0.6
1,029,269 + +1.2
1,029,273 - +0.1
1,029,273 - +0.3
1,029,279 - +0.9
1,029,322 - yccX b0968 0.13 +1.0
1,029,322 - yccX b0968 0.13 -0.7
1,029,343 + yccX b0968 0.20 +0.6
1,029,348 + yccX b0968 0.22 -0.1
1,029,477 - yccX b0968 0.68 -1.0
1,029,477 - yccX b0968 0.68 -0.7
1,029,501 + yccX b0968 0.77 -0.9
1,029,529 - yccX b0968 0.87 +1.9
1,029,529 - yccX b0968 0.87 +0.8
1,029,860 + -1.3
1,029,960 + +0.3
1,029,980 + +1.1
1,029,980 + -0.0
1,030,084 + yccA b0970 0.15 -1.9
1,030,154 + yccA b0970 0.26 +0.9

Or see this region's nucleotide sequence