Experiment: Uridine 0.4095 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ypfI and ypfJ are separated by 14 nucleotides ypfJ and purC are separated by 167 nucleotides
b2474: ypfI - predicted hydrolase (NCBI), at 2,591,866 to 2,593,881
ypfI
b2475: ypfJ - hypothetical protein (NCBI), at 2,593,896 to 2,594,759
ypfJ
b2476: purC - phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI), at 2,594,927 to 2,595,640
purC
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2592.942 kb on + strand, within ypfI at 2592.942 kb on + strand, within ypfI at 2592.942 kb on + strand, within ypfI at 2593.020 kb on - strand, within ypfI at 2593.303 kb on + strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.380 kb on - strand, within ypfI at 2593.394 kb on - strand, within ypfI at 2593.424 kb on - strand, within ypfI at 2593.424 kb on - strand, within ypfI at 2593.509 kb on - strand, within ypfI at 2593.509 kb on - strand, within ypfI at 2593.767 kb on + strand at 2593.775 kb on - strand at 2593.775 kb on - strand at 2593.960 kb on - strand at 2593.973 kb on + strand at 2594.113 kb on - strand, within ypfJ at 2594.113 kb on - strand, within ypfJ at 2594.224 kb on + strand, within ypfJ at 2594.224 kb on + strand, within ypfJ at 2594.282 kb on - strand, within ypfJ at 2594.282 kb on - strand, within ypfJ at 2594.331 kb on - strand, within ypfJ at 2594.338 kb on + strand, within ypfJ at 2594.375 kb on + strand, within ypfJ at 2594.385 kb on - strand, within ypfJ at 2594.430 kb on - strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on + strand, within ypfJ at 2594.449 kb on - strand, within ypfJ at 2594.540 kb on + strand, within ypfJ at 2594.573 kb on + strand, within ypfJ at 2594.608 kb on - strand, within ypfJ at 2594.720 kb on + strand at 2594.872 kb on - strand at 2594.943 kb on - strand at 2594.980 kb on + strand at 2595.179 kb on - strand at 2595.179 kb on - strand, within purC at 2595.364 kb on - strand, within purC at 2595.494 kb on + strand, within purC at 2595.574 kb on + strand at 2595.593 kb on + strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.601 kb on - strand at 2595.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C) remove 2,592,942 + ypfI b2474 0.53 -1.1 2,592,942 + ypfI b2474 0.53 +1.8 2,592,942 + ypfI b2474 0.53 +0.3 2,593,020 - ypfI b2474 0.57 +1.1 2,593,303 + ypfI b2474 0.71 +0.1 2,593,380 - ypfI b2474 0.75 -0.1 2,593,380 - ypfI b2474 0.75 +0.7 2,593,380 - ypfI b2474 0.75 +0.7 2,593,394 - ypfI b2474 0.76 -0.1 2,593,424 - ypfI b2474 0.77 -0.2 2,593,424 - ypfI b2474 0.77 -0.1 2,593,509 - ypfI b2474 0.81 +0.3 2,593,509 - ypfI b2474 0.81 -0.1 2,593,767 + -2.1 2,593,775 - -0.2 2,593,775 - +0.2 2,593,960 - -1.3 2,593,973 + -0.9 2,594,113 - ypfJ b2475 0.25 -1.2 2,594,113 - ypfJ b2475 0.25 -0.9 2,594,224 + ypfJ b2475 0.38 -1.5 2,594,224 + ypfJ b2475 0.38 +0.3 2,594,282 - ypfJ b2475 0.45 -0.3 2,594,282 - ypfJ b2475 0.45 -2.0 2,594,331 - ypfJ b2475 0.50 -2.0 2,594,338 + ypfJ b2475 0.51 -1.0 2,594,375 + ypfJ b2475 0.55 -0.5 2,594,385 - ypfJ b2475 0.57 -0.7 2,594,430 - ypfJ b2475 0.62 -1.0 2,594,449 + ypfJ b2475 0.64 -1.5 2,594,449 + ypfJ b2475 0.64 -1.0 2,594,449 + ypfJ b2475 0.64 -1.2 2,594,449 - ypfJ b2475 0.64 -1.3 2,594,540 + ypfJ b2475 0.75 -0.4 2,594,573 + ypfJ b2475 0.78 -1.3 2,594,608 - ypfJ b2475 0.82 -2.8 2,594,720 + -0.2 2,594,872 - -0.1 2,594,943 - -0.4 2,594,980 + -2.0 2,595,179 - -1.4 2,595,179 - purC b2476 0.35 -1.3 2,595,364 - purC b2476 0.61 -2.2 2,595,494 + purC b2476 0.79 -3.4 2,595,574 + -1.9 2,595,593 + -2.2 2,595,601 - -2.4 2,595,601 - -2.5 2,595,601 - -1.2 2,595,601 - -0.7 2,595,601 - -2.8 2,595,625 - -1.1
Or see this region's nucleotide sequence