Experiment: Uridine 0.4095 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glcF and glcE are separated by 10 nucleotides glcE and glcD overlap by 1 nucleotides
b4467: glcF - glycolate oxidase iron-sulfur subunit (NCBI), at 3,122,258 to 3,123,481
glcF
b4468: glcE - glycolate oxidase FAD binding subunit (NCBI), at 3,123,492 to 3,124,544
glcE
b2979: glcD - glycolate dehydrogenase, putative FAD-linked subunit (from data) , at 3,124,544 to 3,126,043
glcD
Position (kb)
3123
3124
3125 Strain fitness (log2 ratio)
-1
0
1 at 3122.531 kb on + strand, within glcF at 3122.556 kb on + strand, within glcF at 3122.567 kb on + strand, within glcF at 3122.704 kb on + strand, within glcF at 3122.711 kb on - strand, within glcF at 3122.861 kb on + strand, within glcF at 3122.886 kb on - strand, within glcF at 3122.908 kb on - strand, within glcF at 3122.908 kb on - strand, within glcF at 3122.940 kb on + strand, within glcF at 3122.940 kb on + strand, within glcF at 3122.940 kb on + strand, within glcF at 3122.981 kb on + strand, within glcF at 3122.981 kb on + strand, within glcF at 3122.981 kb on + strand, within glcF at 3123.039 kb on - strand, within glcF at 3123.217 kb on + strand, within glcF at 3123.219 kb on + strand, within glcF at 3123.329 kb on - strand, within glcF at 3123.329 kb on - strand, within glcF at 3123.347 kb on - strand, within glcF at 3123.517 kb on + strand at 3123.517 kb on + strand at 3123.555 kb on - strand at 3123.555 kb on - strand at 3123.743 kb on + strand, within glcE at 3123.761 kb on - strand, within glcE at 3123.800 kb on + strand, within glcE at 3123.800 kb on + strand, within glcE at 3123.930 kb on - strand, within glcE at 3123.930 kb on - strand, within glcE at 3123.945 kb on + strand, within glcE at 3124.288 kb on - strand, within glcE at 3124.306 kb on + strand, within glcE at 3124.558 kb on + strand at 3124.558 kb on + strand at 3124.586 kb on + strand at 3124.621 kb on - strand at 3124.800 kb on + strand, within glcD at 3124.953 kb on - strand, within glcD at 3125.135 kb on + strand, within glcD at 3125.135 kb on + strand, within glcD at 3125.139 kb on + strand, within glcD at 3125.146 kb on + strand, within glcD at 3125.243 kb on - strand, within glcD at 3125.243 kb on - strand, within glcD at 3125.263 kb on + strand, within glcD at 3125.263 kb on + strand, within glcD at 3125.297 kb on - strand, within glcD at 3125.297 kb on - strand, within glcD at 3125.518 kb on - strand, within glcD at 3125.518 kb on - strand, within glcD
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C) remove 3,122,531 + glcF b4467 0.22 -0.0 3,122,556 + glcF b4467 0.24 -0.4 3,122,567 + glcF b4467 0.25 -0.2 3,122,704 + glcF b4467 0.36 -0.5 3,122,711 - glcF b4467 0.37 -0.4 3,122,861 + glcF b4467 0.49 -0.2 3,122,886 - glcF b4467 0.51 +0.6 3,122,908 - glcF b4467 0.53 +0.6 3,122,908 - glcF b4467 0.53 -0.1 3,122,940 + glcF b4467 0.56 +0.1 3,122,940 + glcF b4467 0.56 +0.3 3,122,940 + glcF b4467 0.56 +0.4 3,122,981 + glcF b4467 0.59 +0.3 3,122,981 + glcF b4467 0.59 +0.3 3,122,981 + glcF b4467 0.59 -0.8 3,123,039 - glcF b4467 0.64 -0.2 3,123,217 + glcF b4467 0.78 +0.3 3,123,219 + glcF b4467 0.79 -0.4 3,123,329 - glcF b4467 0.88 -0.6 3,123,329 - glcF b4467 0.88 -0.5 3,123,347 - glcF b4467 0.89 +1.3 3,123,517 + +0.2 3,123,517 + +1.4 3,123,555 - +0.4 3,123,555 - -1.4 3,123,743 + glcE b4468 0.24 +0.1 3,123,761 - glcE b4468 0.26 +0.7 3,123,800 + glcE b4468 0.29 -0.3 3,123,800 + glcE b4468 0.29 +0.8 3,123,930 - glcE b4468 0.42 -0.0 3,123,930 - glcE b4468 0.42 +0.9 3,123,945 + glcE b4468 0.43 -0.0 3,124,288 - glcE b4468 0.76 +0.0 3,124,306 + glcE b4468 0.77 +0.1 3,124,558 + -0.2 3,124,558 + -0.3 3,124,586 + +0.0 3,124,621 - -0.6 3,124,800 + glcD b2979 0.17 +1.4 3,124,953 - glcD b2979 0.27 -0.2 3,125,135 + glcD b2979 0.39 +0.0 3,125,135 + glcD b2979 0.39 +0.2 3,125,139 + glcD b2979 0.40 +0.4 3,125,146 + glcD b2979 0.40 +1.1 3,125,243 - glcD b2979 0.47 -0.3 3,125,243 - glcD b2979 0.47 -0.6 3,125,263 + glcD b2979 0.48 +0.4 3,125,263 + glcD b2979 0.48 -0.0 3,125,297 - glcD b2979 0.50 +0.2 3,125,297 - glcD b2979 0.50 -0.1 3,125,518 - glcD b2979 0.65 -0.7 3,125,518 - glcD b2979 0.65 +0.7
Or see this region's nucleotide sequence