Strain Fitness in Escherichia coli BW25113 around b4014

Experiment: Uridine 0.4095 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmetA and aceB are separated by 268 nucleotidesaceB and aceA are separated by 29 nucleotides b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232 metA b4014: aceB - malate synthase (NCBI), at 4,213,501 to 4,215,102 aceB b4015: aceA - isocitrate lyase (NCBI), at 4,215,132 to 4,216,436 aceA Position (kb) 4213 4214 4215 4216Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4212.689 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.957 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.061 kb on - strand, within metAat 4213.068 kb on + strand, within metAat 4213.201 kb on + strandat 4213.491 kb on + strandat 4213.648 kb on + strandat 4213.765 kb on - strand, within aceBat 4213.777 kb on + strand, within aceBat 4213.777 kb on + strand, within aceBat 4213.976 kb on + strand, within aceBat 4214.093 kb on + strand, within aceBat 4214.104 kb on + strand, within aceBat 4214.109 kb on + strand, within aceBat 4214.182 kb on + strand, within aceBat 4214.190 kb on - strand, within aceBat 4214.244 kb on + strand, within aceBat 4214.244 kb on + strand, within aceBat 4214.314 kb on - strand, within aceBat 4214.527 kb on + strand, within aceBat 4214.568 kb on + strand, within aceBat 4214.602 kb on + strand, within aceBat 4214.621 kb on + strand, within aceBat 4214.731 kb on + strand, within aceBat 4214.731 kb on + strand, within aceBat 4214.751 kb on - strand, within aceBat 4214.778 kb on + strand, within aceBat 4215.008 kb on + strandat 4215.037 kb on + strandat 4215.077 kb on + strandat 4215.206 kb on + strandat 4215.214 kb on - strandat 4215.214 kb on - strandat 4215.214 kb on - strandat 4215.214 kb on - strandat 4215.214 kb on - strandat 4215.267 kb on + strand, within aceAat 4215.454 kb on - strand, within aceAat 4215.566 kb on + strand, within aceAat 4215.702 kb on + strand, within aceAat 4215.736 kb on - strand, within aceAat 4215.759 kb on + strand, within aceAat 4215.813 kb on + strand, within aceAat 4215.878 kb on - strand, within aceAat 4215.878 kb on - strand, within aceAat 4215.941 kb on - strand, within aceAat 4215.965 kb on + strand, within aceAat 4215.989 kb on - strand, within aceAat 4215.989 kb on - strand, within aceAat 4216.035 kb on + strand, within aceAat 4216.074 kb on + strand, within aceAat 4216.074 kb on + strand, within aceAat 4216.082 kb on - strand, within aceA

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C)
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4,212,689 - metA b4013 0.42 -2.2
4,212,908 - metA b4013 0.65 -4.0
4,212,908 - metA b4013 0.65 -4.1
4,212,957 + metA b4013 0.70 -3.1
4,213,008 + metA b4013 0.76 -3.2
4,213,008 + metA b4013 0.76 -2.4
4,213,008 + metA b4013 0.76 -5.1
4,213,061 - metA b4013 0.82 -1.0
4,213,068 + metA b4013 0.82 -2.7
4,213,201 + -0.4
4,213,491 + +0.5
4,213,648 + +0.1
4,213,765 - aceB b4014 0.16 +0.8
4,213,777 + aceB b4014 0.17 -0.1
4,213,777 + aceB b4014 0.17 +0.2
4,213,976 + aceB b4014 0.30 -0.0
4,214,093 + aceB b4014 0.37 +0.6
4,214,104 + aceB b4014 0.38 -0.1
4,214,109 + aceB b4014 0.38 +0.7
4,214,182 + aceB b4014 0.43 +0.2
4,214,190 - aceB b4014 0.43 +0.6
4,214,244 + aceB b4014 0.46 -0.2
4,214,244 + aceB b4014 0.46 +0.1
4,214,314 - aceB b4014 0.51 -0.5
4,214,527 + aceB b4014 0.64 +0.8
4,214,568 + aceB b4014 0.67 +0.5
4,214,602 + aceB b4014 0.69 +0.2
4,214,621 + aceB b4014 0.70 +0.2
4,214,731 + aceB b4014 0.77 +0.0
4,214,731 + aceB b4014 0.77 +0.3
4,214,751 - aceB b4014 0.78 +0.2
4,214,778 + aceB b4014 0.80 +0.4
4,215,008 + +0.3
4,215,037 + +0.8
4,215,077 + +0.6
4,215,206 + +0.1
4,215,214 - +0.2
4,215,214 - -0.7
4,215,214 - +0.3
4,215,214 - +1.6
4,215,214 - +0.3
4,215,267 + aceA b4015 0.10 -0.3
4,215,454 - aceA b4015 0.25 +0.6
4,215,566 + aceA b4015 0.33 +1.1
4,215,702 + aceA b4015 0.44 +0.1
4,215,736 - aceA b4015 0.46 +0.2
4,215,759 + aceA b4015 0.48 -0.2
4,215,813 + aceA b4015 0.52 -0.1
4,215,878 - aceA b4015 0.57 -0.5
4,215,878 - aceA b4015 0.57 -0.0
4,215,941 - aceA b4015 0.62 +0.3
4,215,965 + aceA b4015 0.64 +0.3
4,215,989 - aceA b4015 0.66 +0.2
4,215,989 - aceA b4015 0.66 -1.1
4,216,035 + aceA b4015 0.69 -0.6
4,216,074 + aceA b4015 0.72 +0.1
4,216,074 + aceA b4015 0.72 -1.1
4,216,082 - aceA b4015 0.73 +0.1

Or see this region's nucleotide sequence