Strain Fitness in Escherichia coli BW25113 around b2475

Experiment: Uridine 0.4095 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntypfI and ypfJ are separated by 14 nucleotidesypfJ and purC are separated by 167 nucleotides b2474: ypfI - predicted hydrolase (NCBI), at 2,591,866 to 2,593,881 ypfI b2475: ypfJ - hypothetical protein (NCBI), at 2,593,896 to 2,594,759 ypfJ b2476: purC - phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI), at 2,594,927 to 2,595,640 purC Position (kb) 2593 2594 2595Strain fitness (log2 ratio) -3 -2 -1 0 1at 2592.942 kb on + strand, within ypfIat 2592.942 kb on + strand, within ypfIat 2592.942 kb on + strand, within ypfIat 2593.020 kb on - strand, within ypfIat 2593.303 kb on + strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.380 kb on - strand, within ypfIat 2593.394 kb on - strand, within ypfIat 2593.424 kb on - strand, within ypfIat 2593.424 kb on - strand, within ypfIat 2593.509 kb on - strand, within ypfIat 2593.509 kb on - strand, within ypfIat 2593.767 kb on + strandat 2593.775 kb on - strandat 2593.775 kb on - strandat 2593.960 kb on - strandat 2593.973 kb on + strandat 2594.113 kb on - strand, within ypfJat 2594.113 kb on - strand, within ypfJat 2594.224 kb on + strand, within ypfJat 2594.224 kb on + strand, within ypfJat 2594.282 kb on - strand, within ypfJat 2594.282 kb on - strand, within ypfJat 2594.331 kb on - strand, within ypfJat 2594.338 kb on + strand, within ypfJat 2594.375 kb on + strand, within ypfJat 2594.385 kb on - strand, within ypfJat 2594.430 kb on - strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on + strand, within ypfJat 2594.449 kb on - strand, within ypfJat 2594.540 kb on + strand, within ypfJat 2594.573 kb on + strand, within ypfJat 2594.608 kb on - strand, within ypfJat 2594.720 kb on + strandat 2594.872 kb on - strandat 2594.943 kb on - strandat 2594.980 kb on + strandat 2595.179 kb on - strandat 2595.179 kb on - strand, within purCat 2595.364 kb on - strand, within purCat 2595.494 kb on + strand, within purCat 2595.574 kb on + strandat 2595.593 kb on + strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.625 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C)
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2,592,942 + ypfI b2474 0.53 -0.3
2,592,942 + ypfI b2474 0.53 -1.4
2,592,942 + ypfI b2474 0.53 -1.0
2,593,020 - ypfI b2474 0.57 -0.3
2,593,303 + ypfI b2474 0.71 +0.2
2,593,380 - ypfI b2474 0.75 +0.2
2,593,380 - ypfI b2474 0.75 +1.1
2,593,380 - ypfI b2474 0.75 +0.6
2,593,394 - ypfI b2474 0.76 -0.7
2,593,424 - ypfI b2474 0.77 +0.0
2,593,424 - ypfI b2474 0.77 -0.1
2,593,509 - ypfI b2474 0.81 +0.5
2,593,509 - ypfI b2474 0.81 +0.8
2,593,767 + -0.2
2,593,775 - -0.4
2,593,775 - +0.6
2,593,960 - -2.4
2,593,973 + -1.7
2,594,113 - ypfJ b2475 0.25 -2.0
2,594,113 - ypfJ b2475 0.25 -1.0
2,594,224 + ypfJ b2475 0.38 -0.6
2,594,224 + ypfJ b2475 0.38 -1.4
2,594,282 - ypfJ b2475 0.45 -1.8
2,594,282 - ypfJ b2475 0.45 -1.1
2,594,331 - ypfJ b2475 0.50 -1.7
2,594,338 + ypfJ b2475 0.51 -0.7
2,594,375 + ypfJ b2475 0.55 -1.4
2,594,385 - ypfJ b2475 0.57 -1.9
2,594,430 - ypfJ b2475 0.62 -1.5
2,594,449 + ypfJ b2475 0.64 -0.9
2,594,449 + ypfJ b2475 0.64 -0.7
2,594,449 + ypfJ b2475 0.64 -0.2
2,594,449 - ypfJ b2475 0.64 -1.2
2,594,540 + ypfJ b2475 0.75 -1.6
2,594,573 + ypfJ b2475 0.78 -0.6
2,594,608 - ypfJ b2475 0.82 -1.1
2,594,720 + -1.3
2,594,872 - +0.2
2,594,943 - +0.6
2,594,980 + -2.0
2,595,179 - -1.5
2,595,179 - purC b2476 0.35 -1.4
2,595,364 - purC b2476 0.61 -2.4
2,595,494 + purC b2476 0.79 -3.1
2,595,574 + -2.6
2,595,593 + -3.0
2,595,601 - -2.2
2,595,601 - -2.9
2,595,601 - -3.1
2,595,601 - -3.1
2,595,601 - -1.6
2,595,625 - -1.3

Or see this region's nucleotide sequence