Strain Fitness in Escherichia coli BW25113 around b1333

Experiment: Uridine 0.4095 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsH-4 and ynaJ are separated by 272 nucleotidesynaJ and uspE are separated by 49 nucleotidesuspE and fnr are separated by 151 nucleotides b1331: insH-4 - IS5 transposase and trans-activator (NCBI), at 1,394,100 to 1,395,116 insH-4 b1332: ynaJ - predicted inner membrane protein (NCBI), at 1,395,389 to 1,395,646 ynaJ b1333: uspE - stress-induced protein (NCBI), at 1,395,696 to 1,396,646 uspE b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550 fnr Position (kb) 1395 1396 1397Strain fitness (log2 ratio) -1 0 1at 1395.516 kb on - strand, within ynaJat 1395.618 kb on - strand, within ynaJat 1395.682 kb on + strandat 1395.748 kb on - strandat 1396.148 kb on + strand, within uspEat 1396.214 kb on - strand, within uspEat 1396.295 kb on - strand, within uspEat 1396.341 kb on + strand, within uspEat 1396.604 kb on + strandat 1396.609 kb on + strandat 1396.609 kb on + strandat 1396.728 kb on - strandat 1396.728 kb on - strandat 1397.086 kb on + strand, within fnrat 1397.086 kb on + strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.287 kb on - strand, within fnrat 1397.605 kb on + strandat 1397.605 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine 0.4095 mM (C)
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1,395,516 - ynaJ b1332 0.49 +0.4
1,395,618 - ynaJ b1332 0.89 -0.7
1,395,682 + +0.3
1,395,748 - +0.1
1,396,148 + uspE b1333 0.48 +0.9
1,396,214 - uspE b1333 0.54 -0.4
1,396,295 - uspE b1333 0.63 +0.1
1,396,341 + uspE b1333 0.68 -0.5
1,396,604 + -0.4
1,396,609 + -0.9
1,396,609 + -0.6
1,396,728 - +0.2
1,396,728 - +1.2
1,397,086 + fnr b1334 0.38 -0.6
1,397,086 + fnr b1334 0.38 -0.6
1,397,125 - fnr b1334 0.43 -0.2
1,397,125 - fnr b1334 0.43 -0.6
1,397,287 - fnr b1334 0.65 -0.7
1,397,605 + -0.2
1,397,605 + -0.2

Or see this region's nucleotide sequence