Strain Fitness in Escherichia coli BW25113 around b2127

Experiment: Inosine 0.3728 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehU and mlrA are separated by 221 nucleotidesmlrA and yohO are separated by 59 nucleotidesyohO and yehW overlap by 20 nucleotidesyehW and yehX are separated by 4 nucleotides b2126: yehU - predicted sensory kinase in two-component system with YehT (NCBI), at 2,210,981 to 2,212,666 yehU b2127: mlrA - DNA-binding transcriptional regulator (NCBI), at 2,212,888 to 2,213,619 mlrA b4542: yohO - hypothetical protein (NCBI), at 2,213,679 to 2,213,786 yohO b2128: yehW - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,213,767 to 2,214,498 yehW b2129: yehX - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 2,214,503 to 2,215,429 yehX Position (kb) 2212 2213 2214Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 2211.961 kb on - strand, within yehUat 2211.972 kb on - strand, within yehUat 2211.972 kb on - strand, within yehUat 2212.028 kb on + strand, within yehUat 2212.168 kb on + strand, within yehUat 2212.177 kb on - strand, within yehUat 2212.177 kb on - strand, within yehUat 2212.256 kb on - strand, within yehUat 2212.264 kb on - strand, within yehUat 2212.294 kb on + strand, within yehUat 2212.358 kb on - strand, within yehUat 2212.358 kb on - strand, within yehUat 2212.365 kb on + strand, within yehUat 2212.373 kb on - strand, within yehUat 2212.438 kb on - strand, within yehUat 2212.438 kb on - strand, within yehUat 2212.564 kb on + strandat 2213.000 kb on + strand, within mlrAat 2213.008 kb on - strand, within mlrAat 2213.008 kb on - strand, within mlrAat 2213.066 kb on - strand, within mlrAat 2213.222 kb on - strand, within mlrAat 2213.244 kb on + strand, within mlrAat 2213.370 kb on + strand, within mlrAat 2213.370 kb on + strand, within mlrAat 2213.444 kb on + strand, within mlrAat 2213.484 kb on + strand, within mlrAat 2213.506 kb on + strand, within mlrAat 2213.506 kb on + strand, within mlrAat 2213.513 kb on + strand, within mlrAat 2213.641 kb on + strandat 2213.641 kb on + strandat 2213.646 kb on + strandat 2213.654 kb on - strandat 2213.684 kb on - strandat 2213.781 kb on + strandat 2213.819 kb on + strandat 2213.903 kb on - strand, within yehWat 2213.988 kb on - strand, within yehWat 2214.022 kb on - strand, within yehWat 2214.159 kb on + strand, within yehWat 2214.356 kb on - strand, within yehWat 2214.356 kb on - strand, within yehWat 2214.491 kb on + strandat 2214.491 kb on + strandat 2214.540 kb on + strandat 2214.540 kb on + strandat 2214.554 kb on - strandat 2214.568 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Inosine 0.3728 mM (C)
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2,211,961 - yehU b2126 0.58 +0.2
2,211,972 - yehU b2126 0.59 -0.7
2,211,972 - yehU b2126 0.59 -0.5
2,212,028 + yehU b2126 0.62 -0.4
2,212,168 + yehU b2126 0.70 -0.7
2,212,177 - yehU b2126 0.71 -0.2
2,212,177 - yehU b2126 0.71 -0.0
2,212,256 - yehU b2126 0.76 -1.0
2,212,264 - yehU b2126 0.76 +0.8
2,212,294 + yehU b2126 0.78 +0.4
2,212,358 - yehU b2126 0.82 -0.4
2,212,358 - yehU b2126 0.82 +0.5
2,212,365 + yehU b2126 0.82 +0.1
2,212,373 - yehU b2126 0.83 +0.3
2,212,438 - yehU b2126 0.86 +0.1
2,212,438 - yehU b2126 0.86 -0.3
2,212,564 + -0.8
2,213,000 + mlrA b2127 0.15 +4.8
2,213,008 - mlrA b2127 0.16 +3.8
2,213,008 - mlrA b2127 0.16 +2.9
2,213,066 - mlrA b2127 0.24 +4.6
2,213,222 - mlrA b2127 0.46 +3.8
2,213,244 + mlrA b2127 0.49 +3.6
2,213,370 + mlrA b2127 0.66 +4.4
2,213,370 + mlrA b2127 0.66 +3.8
2,213,444 + mlrA b2127 0.76 +4.5
2,213,484 + mlrA b2127 0.81 +3.3
2,213,506 + mlrA b2127 0.84 +4.1
2,213,506 + mlrA b2127 0.84 +4.1
2,213,513 + mlrA b2127 0.85 +4.6
2,213,641 + -1.3
2,213,641 + +0.9
2,213,646 + +1.3
2,213,654 - -0.1
2,213,684 - +0.2
2,213,781 + -0.6
2,213,819 + -0.5
2,213,903 - yehW b2128 0.19 +0.5
2,213,988 - yehW b2128 0.30 +0.7
2,214,022 - yehW b2128 0.35 -0.0
2,214,159 + yehW b2128 0.54 +0.6
2,214,356 - yehW b2128 0.80 -0.0
2,214,356 - yehW b2128 0.80 -0.0
2,214,491 + +0.1
2,214,491 + -1.2
2,214,540 + +0.9
2,214,540 + -0.2
2,214,554 - +0.7
2,214,568 - +0.1

Or see this region's nucleotide sequence