Experiment: Inosine 0.3728 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkA and mscL are separated by 129 nucleotides mscL and yhdL overlap by 4 nucleotides yhdL and zntR are separated by 55 nucleotides zntR and yhdN are separated by 10 nucleotides yhdN and rplQ are separated by 106 nucleotides rplQ and rpoA are separated by 40 nucleotides
b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916
trkA
b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456
mscL
b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671
yhdL
b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152
zntR
b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531
yhdN
b3294: rplQ - 50S ribosomal protein L17 (NCBI), at 3,437,638 to 3,438,021
rplQ
b3295: rpoA - DNA-directed RNA polymerase subunit alpha (NCBI), at 3,438,062 to 3,439,051
rpoA
Position (kb)
3436
3437
3438 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3435.809 kb on + strand at 3435.840 kb on + strand at 3435.840 kb on + strand at 3435.842 kb on + strand at 3435.877 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.903 kb on + strand at 3436.020 kb on - strand at 3436.020 kb on - strand at 3436.020 kb on - strand at 3436.026 kb on + strand at 3436.027 kb on + strand at 3436.027 kb on + strand at 3436.037 kb on - strand at 3436.037 kb on - strand at 3436.045 kb on - strand at 3436.047 kb on - strand at 3436.061 kb on - strand at 3436.071 kb on + strand at 3436.071 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.106 kb on - strand, within mscL at 3436.114 kb on + strand, within mscL at 3436.146 kb on + strand, within mscL at 3436.165 kb on + strand, within mscL at 3436.165 kb on + strand, within mscL at 3436.175 kb on + strand, within mscL at 3436.211 kb on - strand, within mscL at 3436.211 kb on - strand, within mscL at 3436.218 kb on + strand, within mscL at 3436.220 kb on + strand, within mscL at 3436.243 kb on - strand, within mscL at 3436.245 kb on - strand, within mscL at 3436.245 kb on - strand, within mscL at 3436.251 kb on - strand, within mscL at 3436.256 kb on + strand, within mscL at 3436.256 kb on - strand, within mscL at 3436.259 kb on - strand, within mscL at 3436.261 kb on - strand, within mscL at 3436.261 kb on - strand, within mscL at 3436.264 kb on + strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.329 kb on + strand, within mscL at 3436.377 kb on + strand, within mscL at 3436.377 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.398 kb on - strand, within mscL at 3436.429 kb on + strand at 3436.457 kb on - strand at 3436.458 kb on - strand at 3436.460 kb on - strand at 3436.476 kb on + strand, within yhdL at 3436.482 kb on + strand, within yhdL at 3436.482 kb on + strand, within yhdL at 3436.524 kb on - strand, within yhdL at 3436.524 kb on - strand, within yhdL at 3436.528 kb on - strand, within yhdL at 3436.528 kb on - strand, within yhdL at 3436.551 kb on + strand, within yhdL at 3436.551 kb on + strand, within yhdL at 3436.559 kb on - strand, within yhdL at 3436.617 kb on - strand, within yhdL at 3436.636 kb on - strand, within yhdL at 3436.642 kb on - strand, within yhdL at 3436.644 kb on + strand, within yhdL at 3436.659 kb on + strand at 3436.748 kb on + strand at 3436.757 kb on + strand at 3436.767 kb on + strand at 3436.796 kb on - strand, within zntR at 3436.803 kb on + strand, within zntR at 3436.811 kb on - strand, within zntR at 3436.811 kb on - strand, within zntR at 3436.840 kb on + strand, within zntR at 3436.840 kb on + strand, within zntR at 3436.848 kb on + strand, within zntR at 3436.889 kb on + strand, within zntR at 3436.889 kb on + strand, within zntR at 3436.927 kb on + strand, within zntR at 3436.935 kb on - strand, within zntR at 3436.935 kb on - strand, within zntR at 3436.987 kb on - strand, within zntR at 3436.994 kb on - strand, within zntR at 3436.994 kb on - strand, within zntR at 3437.070 kb on - strand, within zntR at 3437.111 kb on - strand at 3437.111 kb on - strand at 3437.158 kb on - strand at 3437.158 kb on - strand at 3437.166 kb on - strand at 3437.223 kb on + strand, within yhdN at 3437.226 kb on + strand, within yhdN at 3437.234 kb on - strand, within yhdN at 3437.276 kb on + strand, within yhdN at 3437.338 kb on + strand, within yhdN at 3437.425 kb on - strand, within yhdN at 3437.425 kb on - strand, within yhdN at 3437.492 kb on + strand, within yhdN at 3437.500 kb on - strand at 3437.500 kb on - strand at 3437.502 kb on - strand at 3437.504 kb on + strand at 3437.529 kb on + strand at 3437.534 kb on - strand at 3437.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Inosine 0.3728 mM (C) remove 3,435,809 + -0.1 3,435,840 + +2.1 3,435,840 + +0.4 3,435,842 + +0.4 3,435,877 + -0.3 3,435,889 + -0.7 3,435,889 + -0.1 3,435,889 + -1.0 3,435,889 + +0.3 3,435,903 + +0.8 3,436,020 - -1.0 3,436,020 - -0.3 3,436,020 - -0.8 3,436,026 + -0.2 3,436,027 + -0.1 3,436,027 + +0.2 3,436,037 - -0.4 3,436,037 - +0.3 3,436,045 - -0.4 3,436,047 - +0.3 3,436,061 - -1.0 3,436,071 + -1.0 3,436,071 + +0.1 3,436,081 + -0.3 3,436,081 + -0.2 3,436,081 + -0.2 3,436,081 + -1.7 3,436,106 - mscL b3291 0.15 +0.5 3,436,114 + mscL b3291 0.17 +1.3 3,436,146 + mscL b3291 0.24 -0.3 3,436,165 + mscL b3291 0.29 -0.8 3,436,165 + mscL b3291 0.29 +1.7 3,436,175 + mscL b3291 0.31 +0.1 3,436,211 - mscL b3291 0.40 -0.6 3,436,211 - mscL b3291 0.40 +0.8 3,436,218 + mscL b3291 0.42 -1.3 3,436,220 + mscL b3291 0.42 -0.7 3,436,243 - mscL b3291 0.48 +0.3 3,436,245 - mscL b3291 0.48 +0.6 3,436,245 - mscL b3291 0.48 +1.3 3,436,251 - mscL b3291 0.50 -0.8 3,436,256 + mscL b3291 0.51 +1.1 3,436,256 - mscL b3291 0.51 +0.0 3,436,259 - mscL b3291 0.52 -0.3 3,436,261 - mscL b3291 0.52 +0.4 3,436,261 - mscL b3291 0.52 +0.3 3,436,264 + mscL b3291 0.53 -0.1 3,436,264 - mscL b3291 0.53 -0.3 3,436,264 - mscL b3291 0.53 +1.9 3,436,264 - mscL b3291 0.53 +0.8 3,436,329 + mscL b3291 0.69 -0.2 3,436,377 + mscL b3291 0.81 -1.6 3,436,377 + mscL b3291 0.81 +0.3 3,436,380 + mscL b3291 0.81 -0.2 3,436,380 + mscL b3291 0.81 -0.9 3,436,380 + mscL b3291 0.81 -1.2 3,436,380 + mscL b3291 0.81 -0.0 3,436,380 + mscL b3291 0.81 +0.7 3,436,398 - mscL b3291 0.86 -1.1 3,436,429 + +0.1 3,436,457 - +1.2 3,436,458 - -0.3 3,436,460 - +2.2 3,436,476 + yhdL b4550 0.11 -0.9 3,436,482 + yhdL b4550 0.13 -1.0 3,436,482 + yhdL b4550 0.13 +0.6 3,436,524 - yhdL b4550 0.32 +0.2 3,436,524 - yhdL b4550 0.32 -0.5 3,436,528 - yhdL b4550 0.34 +0.3 3,436,528 - yhdL b4550 0.34 -0.7 3,436,551 + yhdL b4550 0.45 +0.9 3,436,551 + yhdL b4550 0.45 -0.8 3,436,559 - yhdL b4550 0.48 +0.3 3,436,617 - yhdL b4550 0.75 -1.5 3,436,636 - yhdL b4550 0.84 -0.5 3,436,642 - yhdL b4550 0.86 +0.2 3,436,644 + yhdL b4550 0.87 +0.9 3,436,659 + -0.0 3,436,748 + +1.7 3,436,757 + -0.6 3,436,767 + +0.8 3,436,796 - zntR b3292 0.16 +1.6 3,436,803 + zntR b3292 0.18 +0.6 3,436,811 - zntR b3292 0.20 -0.0 3,436,811 - zntR b3292 0.20 +0.6 3,436,840 + zntR b3292 0.27 -0.9 3,436,840 + zntR b3292 0.27 +0.9 3,436,848 + zntR b3292 0.28 +1.2 3,436,889 + zntR b3292 0.38 +0.1 3,436,889 + zntR b3292 0.38 +0.9 3,436,927 + zntR b3292 0.47 +3.9 3,436,935 - zntR b3292 0.49 -1.1 3,436,935 - zntR b3292 0.49 -1.8 3,436,987 - zntR b3292 0.61 +0.6 3,436,994 - zntR b3292 0.63 -0.6 3,436,994 - zntR b3292 0.63 -0.8 3,437,070 - zntR b3292 0.81 -1.4 3,437,111 - -1.3 3,437,111 - -0.6 3,437,158 - -0.5 3,437,158 - -1.0 3,437,166 - +0.1 3,437,223 + yhdN b3293 0.16 +0.7 3,437,226 + yhdN b3293 0.17 -0.7 3,437,234 - yhdN b3293 0.19 -1.2 3,437,276 + yhdN b3293 0.31 +0.3 3,437,338 + yhdN b3293 0.47 -0.0 3,437,425 - yhdN b3293 0.71 -0.0 3,437,425 - yhdN b3293 0.71 +0.3 3,437,492 + yhdN b3293 0.89 -0.2 3,437,500 - -0.4 3,437,500 - -0.5 3,437,502 - -1.0 3,437,504 + +0.1 3,437,529 + +0.3 3,437,534 - -0.3 3,437,669 - -0.8
Or see this region's nucleotide sequence