Strain Fitness in Escherichia coli BW25113 around b1454

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntansP and yncG are separated by 266 nucleotidesyncG and yncH are separated by 75 nucleotides b1453: ansP - L-asparagine permease (VIMSS), at 1,522,505 to 1,524,004 ansP b1454: yncG - predicted enzyme (NCBI), at 1,524,271 to 1,524,888 yncG b1455: yncH - hypothetical protein (NCBI), at 1,524,964 to 1,525,176 yncH Position (kb) 1524 1525Strain fitness (log2 ratio) -1 0 1at 1523.330 kb on - strand, within ansPat 1523.402 kb on - strand, within ansPat 1523.402 kb on - strand, within ansPat 1523.604 kb on + strand, within ansPat 1523.604 kb on + strand, within ansPat 1523.629 kb on + strand, within ansPat 1523.902 kb on - strandat 1524.042 kb on - strandat 1524.042 kb on - strandat 1524.133 kb on - strandat 1524.133 kb on - strandat 1524.232 kb on - strandat 1524.235 kb on - strandat 1524.242 kb on - strandat 1524.288 kb on - strandat 1524.290 kb on - strandat 1524.432 kb on + strand, within yncGat 1524.472 kb on + strand, within yncGat 1524.574 kb on + strand, within yncGat 1524.609 kb on + strand, within yncGat 1524.632 kb on + strand, within yncGat 1524.704 kb on + strand, within yncGat 1524.753 kb on + strand, within yncGat 1524.753 kb on + strand, within yncGat 1524.763 kb on + strand, within yncGat 1524.763 kb on + strand, within yncGat 1524.768 kb on + strand, within yncG

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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1,523,330 - ansP b1453 0.55 -0.6
1,523,402 - ansP b1453 0.60 +0.9
1,523,402 - ansP b1453 0.60 -0.2
1,523,604 + ansP b1453 0.73 +0.8
1,523,604 + ansP b1453 0.73 -0.5
1,523,629 + ansP b1453 0.75 +0.4
1,523,902 - -0.3
1,524,042 - -0.0
1,524,042 - -0.2
1,524,133 - -0.4
1,524,133 - -1.5
1,524,232 - -0.3
1,524,235 - +0.0
1,524,242 - +0.9
1,524,288 - +0.7
1,524,290 - +0.3
1,524,432 + yncG b1454 0.26 -1.2
1,524,472 + yncG b1454 0.33 -0.8
1,524,574 + yncG b1454 0.49 -0.7
1,524,609 + yncG b1454 0.55 +1.5
1,524,632 + yncG b1454 0.58 +1.1
1,524,704 + yncG b1454 0.70 +0.3
1,524,753 + yncG b1454 0.78 -1.0
1,524,753 + yncG b1454 0.78 -0.7
1,524,763 + yncG b1454 0.80 -0.5
1,524,763 + yncG b1454 0.80 +0.9
1,524,768 + yncG b1454 0.80 -0.1

Or see this region's nucleotide sequence