Strain Fitness in Escherichia coli BW25113 around b0964

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthelD and mgsA are separated by 31 nucleotidesmgsA and yccT are separated by 95 nucleotidesyccT and b0965 are separated by 172 nucleotidesb0965 and hspQ are separated by 44 nucleotides b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748 helD b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238 mgsA b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965 b0966: hspQ - DNA-binding protein, hemimethylated (RefSeq), at 1,027,627 to 1,027,944 hspQ Position (kb) 1026 1027Strain fitness (log2 ratio) -2 -1 0 1 2at 1025.399 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.661 kb on + strandat 1025.662 kb on + strandat 1025.669 kb on - strandat 1025.772 kb on + strandat 1025.827 kb on - strand, within mgsAat 1025.893 kb on - strand, within mgsAat 1025.921 kb on + strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1026.042 kb on - strand, within mgsAat 1026.043 kb on - strandat 1026.054 kb on + strand, within mgsAat 1026.115 kb on + strand, within mgsAat 1026.116 kb on - strand, within mgsAat 1026.119 kb on + strand, within mgsAat 1026.123 kb on - strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.295 kb on + strandat 1026.403 kb on - strand, within yccTat 1026.403 kb on - strand, within yccTat 1026.627 kb on + strand, within yccTat 1026.627 kb on + strandat 1026.685 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.804 kb on + strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.958 kb on + strandat 1026.985 kb on + strandat 1026.985 kb on + strandat 1026.992 kb on + strandat 1027.060 kb on + strandat 1027.060 kb on + strandat 1027.085 kb on + strandat 1027.145 kb on + strandat 1027.153 kb on - strandat 1027.293 kb on - strand, within b0965at 1027.466 kb on + strand, within b0965at 1027.570 kb on - strandat 1027.626 kb on - strandat 1027.633 kb on - strandat 1027.658 kb on - strandat 1027.667 kb on + strand, within hspQat 1027.674 kb on - strand, within hspQat 1027.675 kb on - strand, within hspQat 1027.695 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.755 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.885 kb on + strand, within hspQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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1,025,399 + helD b0962 0.83 -0.3
1,025,424 + helD b0962 0.84 +1.2
1,025,424 + helD b0962 0.84 +0.1
1,025,661 + -0.6
1,025,662 + -0.3
1,025,669 - +0.2
1,025,772 + -0.2
1,025,827 - mgsA b0963 0.10 -1.2
1,025,893 - mgsA b0963 0.25 -0.7
1,025,921 + mgsA b0963 0.31 -0.1
1,025,943 - mgsA b0963 0.36 -2.0
1,025,943 - mgsA b0963 0.36 -1.2
1,026,042 - mgsA b0963 0.57 -0.1
1,026,043 - +1.3
1,026,054 + mgsA b0963 0.60 -1.9
1,026,115 + mgsA b0963 0.73 -0.1
1,026,116 - mgsA b0963 0.73 -0.9
1,026,119 + mgsA b0963 0.74 -0.2
1,026,123 - mgsA b0963 0.75 +0.5
1,026,153 + mgsA b0963 0.81 -0.6
1,026,153 + mgsA b0963 0.81 -0.3
1,026,295 + +0.6
1,026,403 - yccT b0964 0.10 -0.2
1,026,403 - yccT b0964 0.10 -0.9
1,026,627 + yccT b0964 0.44 -1.8
1,026,627 + +1.9
1,026,685 + yccT b0964 0.53 -0.8
1,026,740 + yccT b0964 0.61 -2.0
1,026,740 + yccT b0964 0.61 -1.2
1,026,804 + yccT b0964 0.71 -0.8
1,026,824 - yccT b0964 0.74 +0.2
1,026,824 - yccT b0964 0.74 -0.4
1,026,958 + -1.0
1,026,985 + -0.5
1,026,985 + +0.1
1,026,992 + -0.8
1,027,060 + -0.8
1,027,060 + -0.4
1,027,085 + -1.0
1,027,145 + -0.6
1,027,153 - +0.5
1,027,293 - b0965 b0965 0.30 +0.3
1,027,466 + b0965 b0965 0.72 -0.2
1,027,570 - -1.7
1,027,626 - +0.4
1,027,633 - +0.4
1,027,658 - -0.5
1,027,667 + hspQ b0966 0.13 -0.7
1,027,674 - hspQ b0966 0.15 -0.4
1,027,675 - hspQ b0966 0.15 -1.4
1,027,695 + hspQ b0966 0.21 -0.4
1,027,747 + hspQ b0966 0.38 +1.3
1,027,747 + hspQ b0966 0.38 -1.6
1,027,755 - hspQ b0966 0.40 -0.4
1,027,810 - hspQ b0966 0.58 +0.1
1,027,810 - hspQ b0966 0.58 -0.3
1,027,865 - hspQ b0966 0.75 -0.1
1,027,865 - hspQ b0966 0.75 -0.8
1,027,885 + hspQ b0966 0.81 +0.6

Or see this region's nucleotide sequence