Strain Fitness in Escherichia coli BW25113 around b2214

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntada and yojL are separated by 73 nucleotidesyojL and ompC are separated by 111 nucleotides b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427 ada b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556 yojL b2215: ompC - outer membrane porin protein C (NCBI), at 2,309,668 to 2,310,771 ompC Position (kb) 2308 2309 2310Strain fitness (log2 ratio) -1 0 1 2 3at 2307.549 kb on - strand, within adaat 2307.562 kb on - strand, within adaat 2307.583 kb on + strand, within adaat 2307.593 kb on + strand, within adaat 2307.849 kb on - strand, within adaat 2308.153 kb on + strand, within adaat 2308.153 kb on + strand, within adaat 2308.162 kb on + strand, within adaat 2308.327 kb on + strandat 2308.337 kb on - strandat 2308.651 kb on + strand, within yojLat 2308.661 kb on - strandat 2308.661 kb on - strand, within yojLat 2308.745 kb on - strand, within yojLat 2308.784 kb on - strand, within yojLat 2308.796 kb on - strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.916 kb on + strand, within yojLat 2308.964 kb on - strand, within yojLat 2308.964 kb on - strand, within yojLat 2309.070 kb on - strand, within yojLat 2309.100 kb on + strand, within yojLat 2309.123 kb on - strand, within yojLat 2309.286 kb on - strand, within yojLat 2309.286 kb on - strand, within yojLat 2309.711 kb on - strandat 2309.963 kb on + strand, within ompCat 2309.981 kb on + strand, within ompCat 2310.013 kb on + strand, within ompCat 2310.044 kb on + strand, within ompCat 2310.052 kb on - strand, within ompCat 2310.052 kb on - strandat 2310.081 kb on - strand, within ompCat 2310.091 kb on - strand, within ompCat 2310.155 kb on + strand, within ompCat 2310.192 kb on + strand, within ompCat 2310.277 kb on + strand, within ompCat 2310.340 kb on + strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.362 kb on - strand, within ompCat 2310.442 kb on + strand, within ompCat 2310.442 kb on + strand, within ompCat 2310.490 kb on + strand, within ompCat 2310.531 kb on + strand, within ompCat 2310.532 kb on + strand, within ompCat 2310.538 kb on - strand, within ompCat 2310.538 kb on - strand, within ompC

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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2,307,549 - ada b2213 0.17 -1.1
2,307,562 - ada b2213 0.19 +1.0
2,307,583 + ada b2213 0.21 -1.4
2,307,593 + ada b2213 0.22 -0.2
2,307,849 - ada b2213 0.46 -0.3
2,308,153 + ada b2213 0.74 -0.1
2,308,153 + ada b2213 0.74 +0.2
2,308,162 + ada b2213 0.75 -0.9
2,308,327 + -0.5
2,308,337 - +0.2
2,308,651 + yojL b2214 0.14 -0.2
2,308,661 - -1.0
2,308,661 - yojL b2214 0.15 -0.3
2,308,745 - yojL b2214 0.23 +0.1
2,308,784 - yojL b2214 0.27 -0.3
2,308,796 - yojL b2214 0.28 +0.7
2,308,916 + yojL b2214 0.39 +0.9
2,308,916 + yojL b2214 0.39 -0.0
2,308,964 - yojL b2214 0.44 -0.0
2,308,964 - yojL b2214 0.44 -0.2
2,309,070 - yojL b2214 0.54 +0.9
2,309,100 + yojL b2214 0.57 +0.4
2,309,123 - yojL b2214 0.59 +0.4
2,309,286 - yojL b2214 0.74 +1.0
2,309,286 - yojL b2214 0.74 -0.3
2,309,711 - -0.1
2,309,963 + ompC b2215 0.27 -0.2
2,309,981 + ompC b2215 0.28 +1.9
2,310,013 + ompC b2215 0.31 +0.5
2,310,044 + ompC b2215 0.34 +0.3
2,310,052 - ompC b2215 0.35 -0.7
2,310,052 - -0.4
2,310,081 - ompC b2215 0.37 -0.6
2,310,091 - ompC b2215 0.38 +0.3
2,310,155 + ompC b2215 0.44 +1.1
2,310,192 + ompC b2215 0.47 +2.9
2,310,277 + ompC b2215 0.55 +0.7
2,310,340 + ompC b2215 0.61 +0.1
2,310,362 - ompC b2215 0.63 -0.7
2,310,362 - ompC b2215 0.63 -0.6
2,310,362 - ompC b2215 0.63 -0.0
2,310,442 + ompC b2215 0.70 -1.3
2,310,442 + ompC b2215 0.70 +0.4
2,310,490 + ompC b2215 0.74 -0.2
2,310,531 + ompC b2215 0.78 -0.3
2,310,532 + ompC b2215 0.78 +0.0
2,310,538 - ompC b2215 0.79 +0.3
2,310,538 - ompC b2215 0.79 -0.7

Or see this region's nucleotide sequence