Strain Fitness in Escherichia coli BW25113 around b0966

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyccT and b0965 are separated by 172 nucleotidesb0965 and hspQ are separated by 44 nucleotideshspQ and yccW are separated by 57 nucleotides b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965 b0966: hspQ - DNA-binding protein, hemimethylated (RefSeq), at 1,027,627 to 1,027,944 hspQ b0967: yccW - predicted methyltransferase (RefSeq), at 1,028,002 to 1,029,192 yccW Position (kb) 1027 1028Strain fitness (log2 ratio) -2 -1 0 1at 1026.627 kb on + strand, within yccTat 1026.627 kb on + strandat 1026.685 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.804 kb on + strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.958 kb on + strandat 1026.985 kb on + strandat 1026.985 kb on + strandat 1026.992 kb on + strandat 1027.060 kb on + strandat 1027.060 kb on + strandat 1027.085 kb on + strandat 1027.145 kb on + strandat 1027.153 kb on - strandat 1027.293 kb on - strand, within b0965at 1027.466 kb on + strand, within b0965at 1027.570 kb on - strandat 1027.626 kb on - strandat 1027.633 kb on - strandat 1027.658 kb on - strandat 1027.667 kb on + strand, within hspQat 1027.674 kb on - strand, within hspQat 1027.675 kb on - strand, within hspQat 1027.695 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.747 kb on + strand, within hspQat 1027.755 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.810 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.865 kb on - strand, within hspQat 1027.885 kb on + strand, within hspQat 1028.057 kb on - strandat 1028.120 kb on + strandat 1028.328 kb on + strand, within yccWat 1028.328 kb on + strand, within yccWat 1028.373 kb on - strand, within yccWat 1028.378 kb on - strand, within yccWat 1028.378 kb on - strand, within yccWat 1028.425 kb on - strand, within yccWat 1028.478 kb on + strand, within yccWat 1028.509 kb on - strand, within yccWat 1028.579 kb on - strand, within yccWat 1028.579 kb on - strand, within yccWat 1028.619 kb on + strand, within yccWat 1028.685 kb on - strand, within yccWat 1028.796 kb on + strand, within yccWat 1028.796 kb on + strand, within yccWat 1028.841 kb on + strand, within yccWat 1028.846 kb on + strand, within yccWat 1028.901 kb on - strand, within yccW

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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1,026,627 + yccT b0964 0.44 -0.6
1,026,627 + +1.6
1,026,685 + yccT b0964 0.53 -1.9
1,026,740 + yccT b0964 0.61 -1.1
1,026,740 + yccT b0964 0.61 -1.6
1,026,804 + yccT b0964 0.71 -0.3
1,026,824 - yccT b0964 0.74 -0.6
1,026,824 - yccT b0964 0.74 -0.7
1,026,958 + -0.5
1,026,985 + -0.8
1,026,985 + -0.6
1,026,992 + -0.9
1,027,060 + -0.6
1,027,060 + -2.7
1,027,085 + -0.8
1,027,145 + +0.9
1,027,153 - +1.0
1,027,293 - b0965 b0965 0.30 -0.9
1,027,466 + b0965 b0965 0.72 -1.1
1,027,570 - +0.2
1,027,626 - +0.4
1,027,633 - +0.3
1,027,658 - -0.1
1,027,667 + hspQ b0966 0.13 -1.0
1,027,674 - hspQ b0966 0.15 -0.1
1,027,675 - hspQ b0966 0.15 +0.2
1,027,695 + hspQ b0966 0.21 -0.8
1,027,747 + hspQ b0966 0.38 +0.6
1,027,747 + hspQ b0966 0.38 -0.1
1,027,755 - hspQ b0966 0.40 -0.3
1,027,810 - hspQ b0966 0.58 -0.7
1,027,810 - hspQ b0966 0.58 -0.8
1,027,865 - hspQ b0966 0.75 -0.4
1,027,865 - hspQ b0966 0.75 +0.0
1,027,885 + hspQ b0966 0.81 +0.5
1,028,057 - +0.2
1,028,120 + -0.7
1,028,328 + yccW b0967 0.27 +0.1
1,028,328 + yccW b0967 0.27 -0.2
1,028,373 - yccW b0967 0.31 -0.7
1,028,378 - yccW b0967 0.32 -1.1
1,028,378 - yccW b0967 0.32 +0.8
1,028,425 - yccW b0967 0.36 +0.3
1,028,478 + yccW b0967 0.40 +0.2
1,028,509 - yccW b0967 0.43 +0.0
1,028,579 - yccW b0967 0.48 -0.5
1,028,579 - yccW b0967 0.48 +0.1
1,028,619 + yccW b0967 0.52 +0.4
1,028,685 - yccW b0967 0.57 -0.1
1,028,796 + yccW b0967 0.67 -0.1
1,028,796 + yccW b0967 0.67 -1.6
1,028,841 + yccW b0967 0.70 -0.1
1,028,846 + yccW b0967 0.71 +0.2
1,028,901 - yccW b0967 0.75 -1.2

Or see this region's nucleotide sequence