Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt exbD and exbB are separated by 6 nucleotides exbB and metC are separated by 251 nucleotides metC and yghB are separated by 139 nucleotides yghB and yqhC are separated by 39 nucleotides
b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265
exbD
b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006
exbB
b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445
metC
b3009: yghB - conserved inner membrane protein (NCBI), at 3,151,585 to 3,152,244
yghB
b3010: yqhC - putative ARAC-type regulatory protein (VIMSS), at 3,152,284 to 3,153,240
yqhC
Position (kb)
3150
3151
3152 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3149.377 kb on + strand, within exbB at 3149.377 kb on + strand, within exbB at 3149.380 kb on + strand, within exbB at 3149.396 kb on - strand, within exbB at 3149.448 kb on - strand, within exbB at 3149.501 kb on + strand, within exbB at 3149.508 kb on + strand, within exbB at 3149.543 kb on - strand, within exbB at 3149.553 kb on - strand, within exbB at 3149.554 kb on + strand, within exbB at 3149.564 kb on + strand, within exbB at 3149.659 kb on + strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.690 kb on - strand, within exbB at 3149.707 kb on + strand, within exbB at 3149.710 kb on - strand, within exbB at 3149.718 kb on + strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.866 kb on + strand, within exbB at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.983 kb on + strand at 3150.016 kb on - strand at 3150.257 kb on - strand at 3150.288 kb on - strand at 3150.292 kb on + strand at 3150.300 kb on - strand at 3150.300 kb on - strand at 3150.337 kb on - strand at 3150.378 kb on - strand, within metC at 3150.421 kb on + strand, within metC at 3150.421 kb on + strand, within metC at 3150.514 kb on - strand, within metC at 3150.575 kb on + strand, within metC at 3150.577 kb on - strand, within metC at 3150.583 kb on - strand, within metC at 3150.640 kb on - strand, within metC at 3150.654 kb on + strand, within metC at 3150.669 kb on + strand, within metC at 3150.706 kb on - strand, within metC at 3150.791 kb on - strand, within metC at 3150.820 kb on + strand, within metC at 3150.820 kb on + strand, within metC at 3150.912 kb on + strand, within metC at 3150.914 kb on + strand, within metC at 3150.915 kb on + strand, within metC at 3150.932 kb on - strand, within metC at 3151.015 kb on + strand at 3151.015 kb on + strand, within metC at 3151.028 kb on + strand, within metC at 3151.038 kb on - strand, within metC at 3151.038 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.120 kb on + strand, within metC at 3151.125 kb on + strand, within metC at 3151.138 kb on - strand, within metC at 3151.216 kb on + strand, within metC at 3151.216 kb on + strand, within metC at 3151.237 kb on + strand, within metC at 3151.274 kb on - strand, within metC at 3151.376 kb on + strand at 3151.655 kb on - strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.867 kb on - strand, within yghB at 3151.889 kb on + strand, within yghB at 3151.889 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.076 kb on + strand, within yghB at 3152.084 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.130 kb on + strand, within yghB at 3152.133 kb on + strand, within yghB at 3152.152 kb on - strand, within yghB at 3152.153 kb on - strand, within yghB at 3152.186 kb on + strand at 3152.256 kb on + strand at 3152.256 kb on + strand at 3152.280 kb on + strand at 3152.290 kb on + strand at 3152.290 kb on + strand at 3152.372 kb on + strand at 3152.377 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 3,149,377 + exbB b3006 0.14 +0.0 3,149,377 + exbB b3006 0.14 -1.8 3,149,380 + exbB b3006 0.15 -0.8 3,149,396 - exbB b3006 0.17 +0.3 3,149,448 - exbB b3006 0.24 +0.5 3,149,501 + exbB b3006 0.31 -1.7 3,149,508 + exbB b3006 0.32 -0.2 3,149,543 - exbB b3006 0.37 -0.4 3,149,553 - exbB b3006 0.38 -0.3 3,149,554 + exbB b3006 0.38 +0.1 3,149,564 + exbB b3006 0.40 +0.3 3,149,659 + exbB b3006 0.53 -0.2 3,149,682 - exbB b3006 0.56 +0.5 3,149,682 - exbB b3006 0.56 -0.3 3,149,690 - exbB b3006 0.57 +0.1 3,149,707 + exbB b3006 0.59 -0.3 3,149,710 - exbB b3006 0.60 -0.9 3,149,718 + exbB b3006 0.61 -0.5 3,149,803 - exbB b3006 0.72 +0.7 3,149,803 - exbB b3006 0.72 -0.4 3,149,866 + exbB b3006 0.81 +0.1 3,149,956 + -0.2 3,149,956 + -0.6 3,149,956 + -1.5 3,149,983 + +1.2 3,150,016 - +0.2 3,150,257 - -0.4 3,150,288 - -0.5 3,150,292 + -2.5 3,150,300 - +0.0 3,150,300 - +0.2 3,150,337 - -2.4 3,150,378 - metC b3008 0.10 -1.4 3,150,421 + metC b3008 0.14 -3.0 3,150,421 + metC b3008 0.14 -0.9 3,150,514 - metC b3008 0.22 -0.8 3,150,575 + metC b3008 0.27 -1.0 3,150,577 - metC b3008 0.27 -1.8 3,150,583 - metC b3008 0.27 -3.4 3,150,640 - metC b3008 0.32 -1.2 3,150,654 + metC b3008 0.33 -0.4 3,150,669 + metC b3008 0.35 -1.0 3,150,706 - metC b3008 0.38 -0.9 3,150,791 - metC b3008 0.45 -1.0 3,150,820 + metC b3008 0.47 -0.7 3,150,820 + metC b3008 0.47 -1.7 3,150,912 + metC b3008 0.55 -0.2 3,150,914 + metC b3008 0.55 -2.0 3,150,915 + metC b3008 0.55 -2.0 3,150,932 - metC b3008 0.57 -0.7 3,151,015 + +0.2 3,151,015 + metC b3008 0.64 -0.4 3,151,028 + metC b3008 0.65 -1.3 3,151,038 - metC b3008 0.66 -3.1 3,151,038 - metC b3008 0.66 -0.5 3,151,074 - metC b3008 0.69 -0.3 3,151,074 - metC b3008 0.69 -1.9 3,151,120 + metC b3008 0.73 -2.1 3,151,125 + metC b3008 0.73 -1.4 3,151,138 - metC b3008 0.74 -0.7 3,151,216 + metC b3008 0.81 -1.0 3,151,216 + metC b3008 0.81 -3.3 3,151,237 + metC b3008 0.82 -1.1 3,151,274 - metC b3008 0.86 -1.8 3,151,376 + -0.5 3,151,655 - yghB b3009 0.11 -0.2 3,151,859 + yghB b3009 0.42 +0.7 3,151,859 + yghB b3009 0.42 +0.4 3,151,867 - yghB b3009 0.43 -1.3 3,151,889 + yghB b3009 0.46 -0.7 3,151,889 + yghB b3009 0.46 -0.4 3,152,046 + yghB b3009 0.70 +0.1 3,152,046 + yghB b3009 0.70 -0.6 3,152,076 + yghB b3009 0.74 -0.1 3,152,084 - yghB b3009 0.76 +0.4 3,152,101 - yghB b3009 0.78 -1.4 3,152,101 - yghB b3009 0.78 -1.6 3,152,130 + yghB b3009 0.83 +0.1 3,152,133 + yghB b3009 0.83 +0.2 3,152,152 - yghB b3009 0.86 +0.6 3,152,153 - yghB b3009 0.86 +0.4 3,152,186 + +0.8 3,152,256 + -0.1 3,152,256 + -0.6 3,152,280 + +0.1 3,152,290 + -1.9 3,152,290 + -0.2 3,152,372 + +0.1 3,152,377 + +1.1
Or see this region's nucleotide sequence