Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaC and hcaB overlap by 4 nucleotides hcaB and hcaD are separated by 9 nucleotides hcaD and yphA are separated by 96 nucleotides yphA and yphB are separated by 47 nucleotides
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271
hcaD
b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790
yphA
b2544: yphB - hypothetical protein (NCBI), at 2,671,838 to 2,672,710
yphB
Position (kb)
2670
2671
2672 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2669.083 kb on + strand, within hcaC at 2669.112 kb on - strand, within hcaC at 2669.166 kb on + strand, within hcaC at 2669.295 kb on + strand at 2669.442 kb on - strand, within hcaB at 2669.469 kb on - strand at 2669.662 kb on + strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.946 kb on + strand, within hcaB at 2669.947 kb on - strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2670.153 kb on + strand at 2670.199 kb on + strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.447 kb on + strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.603 kb on + strand, within hcaD at 2670.836 kb on - strand, within hcaD at 2670.927 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2671.037 kb on + strand, within hcaD at 2671.203 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.323 kb on - strand at 2671.373 kb on - strand at 2671.373 kb on - strand at 2671.494 kb on + strand, within yphA at 2671.494 kb on + strand, within yphA at 2671.496 kb on + strand, within yphA at 2671.497 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.509 kb on + strand, within yphA at 2671.603 kb on + strand, within yphA at 2671.641 kb on - strand, within yphA at 2671.645 kb on - strand, within yphA at 2671.713 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.789 kb on + strand at 2671.890 kb on - strand at 2671.959 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.053 kb on + strand, within yphB at 2672.081 kb on + strand, within yphB at 2672.084 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 2,669,083 + hcaC b2540 0.48 -0.6 2,669,112 - hcaC b2540 0.57 -0.2 2,669,166 + hcaC b2540 0.74 -0.1 2,669,295 + -0.2 2,669,442 - hcaB b2541 0.24 -0.4 2,669,469 - -2.2 2,669,662 + hcaB b2541 0.51 +0.6 2,669,678 - hcaB b2541 0.53 -2.8 2,669,678 - hcaB b2541 0.53 +0.1 2,669,716 - hcaB b2541 0.58 +1.0 2,669,716 - hcaB b2541 0.58 -0.4 2,669,827 + hcaB b2541 0.71 -1.4 2,669,827 + hcaB b2541 0.71 +0.4 2,669,835 - hcaB b2541 0.72 -0.0 2,669,835 - hcaB b2541 0.72 -0.4 2,669,946 + hcaB b2541 0.86 -0.2 2,669,947 - hcaB b2541 0.86 +0.1 2,669,951 + hcaB b2541 0.87 -0.0 2,669,951 + hcaB b2541 0.87 -1.9 2,670,153 + +0.2 2,670,199 + hcaD b2542 0.11 +0.8 2,670,427 - hcaD b2542 0.30 -0.6 2,670,427 - hcaD b2542 0.30 +1.5 2,670,447 + hcaD b2542 0.31 -0.1 2,670,450 - hcaD b2542 0.32 -1.4 2,670,450 - hcaD b2542 0.32 +0.9 2,670,576 - hcaD b2542 0.42 -0.4 2,670,576 - hcaD b2542 0.42 -0.4 2,670,576 - hcaD b2542 0.42 -1.1 2,670,603 + hcaD b2542 0.44 -0.3 2,670,836 - hcaD b2542 0.64 +0.7 2,670,927 - hcaD b2542 0.71 +0.2 2,670,994 - hcaD b2542 0.77 -2.0 2,670,994 - hcaD b2542 0.77 +1.7 2,671,037 + hcaD b2542 0.80 +1.0 2,671,203 + +1.3 2,671,315 + -0.1 2,671,315 + +0.2 2,671,315 + -0.6 2,671,323 - -0.1 2,671,373 - +1.1 2,671,373 - -0.1 2,671,494 + yphA b2543 0.30 -1.1 2,671,494 + yphA b2543 0.30 -0.8 2,671,496 + yphA b2543 0.30 -0.5 2,671,497 - yphA b2543 0.30 +0.1 2,671,502 - yphA b2543 0.32 +1.1 2,671,502 - yphA b2543 0.32 +0.6 2,671,509 + yphA b2543 0.33 +0.1 2,671,603 + yphA b2543 0.56 -0.1 2,671,641 - yphA b2543 0.65 +0.2 2,671,645 - yphA b2543 0.65 -0.1 2,671,713 + yphA b2543 0.82 +0.3 2,671,736 + yphA b2543 0.87 +0.7 2,671,736 + yphA b2543 0.87 -0.2 2,671,789 + -1.8 2,671,890 - +1.0 2,671,959 - yphB b2544 0.14 +0.2 2,672,031 - yphB b2544 0.22 -0.4 2,672,031 - yphB b2544 0.22 +0.4 2,672,053 + yphB b2544 0.25 +0.2 2,672,081 + yphB b2544 0.28 +1.0 2,672,084 - yphB b2544 0.28 -0.4 2,672,165 - yphB b2544 0.37 +0.2 2,672,165 - yphB b2544 0.37 +0.5
Or see this region's nucleotide sequence