Experiment: Guanosine 0.35306 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysK and ptsH are separated by 383 nucleotides ptsH and ptsI are separated by 44 nucleotides ptsI and crr are separated by 40 nucleotides crr and pdxK are separated by 42 nucleotides
b2414: cysK - cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI), at 2,530,431 to 2,531,402
cysK
b2415: ptsH - phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) (NCBI), at 2,531,786 to 2,532,043
ptsH
b2416: ptsI - PEP-protein phosphotransferase of PTS system (enzyme I) (NCBI), at 2,532,088 to 2,533,815
ptsI
b2417: crr - glucose-specific PTS system enzyme IIA component (NCBI), at 2,533,856 to 2,534,365
crr
b2418: pdxK - pyridoxine kinase (NCBI), at 2,534,408 to 2,535,259
pdxK
Position (kb)
2532
2533
2534 Strain fitness (log2 ratio)
-2
-1
0
1 at 2531.138 kb on + strand, within cysK at 2531.138 kb on + strand, within cysK at 2531.169 kb on - strand, within cysK at 2531.314 kb on - strand at 2531.455 kb on + strand at 2531.455 kb on + strand at 2531.478 kb on + strand at 2531.478 kb on + strand at 2531.484 kb on + strand at 2531.666 kb on + strand at 2531.666 kb on + strand at 2531.725 kb on + strand at 2531.725 kb on + strand at 2534.053 kb on - strand, within crr at 2534.468 kb on - strand at 2534.488 kb on + strand at 2534.506 kb on - strand, within pdxK at 2534.519 kb on - strand, within pdxK at 2534.519 kb on - strand, within pdxK at 2534.591 kb on - strand, within pdxK at 2534.591 kb on - strand, within pdxK at 2534.601 kb on + strand, within pdxK at 2534.601 kb on + strand, within pdxK at 2534.618 kb on + strand, within pdxK at 2534.618 kb on + strand, within pdxK at 2534.708 kb on - strand, within pdxK
Per-strain Table
Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C) remove 2,531,138 + cysK b2414 0.73 -1.3 2,531,138 + cysK b2414 0.73 -2.2 2,531,169 - cysK b2414 0.76 -2.1 2,531,314 - -1.6 2,531,455 + -0.0 2,531,455 + -1.1 2,531,478 + +0.3 2,531,478 + +1.0 2,531,484 + +0.4 2,531,666 + -1.4 2,531,666 + +0.8 2,531,725 + -1.8 2,531,725 + +0.6 2,534,053 - crr b2417 0.39 -1.5 2,534,468 - -1.2 2,534,488 + +1.5 2,534,506 - pdxK b2418 0.12 -0.5 2,534,519 - pdxK b2418 0.13 +0.3 2,534,519 - pdxK b2418 0.13 -0.3 2,534,591 - pdxK b2418 0.21 +0.1 2,534,591 - pdxK b2418 0.21 -0.0 2,534,601 + pdxK b2418 0.23 -0.2 2,534,601 + pdxK b2418 0.23 +0.0 2,534,618 + pdxK b2418 0.25 -0.3 2,534,618 + pdxK b2418 0.25 +0.3 2,534,708 - pdxK b2418 0.35 -0.3
Or see this region's nucleotide sequence