Strain Fitness in Escherichia coli BW25113 around b1819

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmanX and manY are separated by 62 nucleotidesmanY and manZ are separated by 12 nucleotidesmanZ and yobD are separated by 54 nucleotidesyobD and b1821 are separated by 428 nucleotides b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043 manX b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906 manY b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770 manZ b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283 yobD b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278 b1821 Position (kb) 1901 1902 1903Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1900.964 kb on + strandat 1900.972 kb on - strandat 1901.109 kb on - strandat 1901.109 kb on - strandat 1901.147 kb on + strandat 1901.150 kb on - strandat 1901.150 kb on - strandat 1901.406 kb on + strand, within manYat 1901.560 kb on - strand, within manYat 1901.794 kb on - strand, within manYat 1902.131 kb on + strand, within manZat 1902.131 kb on + strand, within manZat 1902.139 kb on - strand, within manZat 1902.139 kb on - strand, within manZat 1902.146 kb on + strand, within manZat 1902.425 kb on - strand, within manZat 1902.428 kb on + strand, within manZat 1902.618 kb on + strand, within manZat 1902.618 kb on + strand, within manZat 1902.626 kb on - strand, within manZat 1902.758 kb on + strandat 1902.763 kb on + strandat 1902.765 kb on + strandat 1902.765 kb on - strandat 1902.766 kb on - strandat 1902.773 kb on - strandat 1902.781 kb on - strandat 1902.867 kb on + strandat 1902.867 kb on + strandat 1902.875 kb on - strand, within yobDat 1902.875 kb on - strandat 1902.875 kb on - strand, within yobDat 1902.882 kb on + strand, within yobDat 1902.893 kb on + strand, within yobDat 1902.914 kb on + strand, within yobDat 1902.964 kb on + strand, within yobDat 1902.996 kb on + strand, within yobDat 1903.006 kb on - strand, within yobDat 1903.026 kb on - strand, within yobDat 1903.040 kb on + strand, within yobDat 1903.051 kb on + strand, within yobDat 1903.051 kb on + strand, within yobDat 1903.059 kb on - strand, within yobDat 1903.069 kb on - strand, within yobDat 1903.138 kb on + strand, within yobDat 1903.161 kb on + strand, within yobDat 1903.161 kb on + strand, within yobDat 1903.179 kb on - strand, within yobDat 1903.183 kb on + strand, within yobDat 1903.196 kb on + strand, within yobDat 1903.198 kb on + strand, within yobDat 1903.496 kb on - strandat 1903.498 kb on - strandat 1903.529 kb on + strandat 1903.537 kb on - strandat 1903.548 kb on + strandat 1903.553 kb on + strandat 1903.558 kb on + strandat 1903.558 kb on + strandat 1903.583 kb on + strandat 1903.602 kb on - strandat 1903.602 kb on - strandat 1903.639 kb on - strandat 1903.654 kb on - strandat 1903.702 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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1,900,964 + +1.2
1,900,972 - +0.2
1,901,109 - -0.7
1,901,109 - +0.9
1,901,147 + +0.3
1,901,150 - +0.6
1,901,150 - +0.0
1,901,406 + manY b1818 0.37 +0.7
1,901,560 - manY b1818 0.57 +0.7
1,901,794 - manY b1818 0.86 +1.8
1,902,131 + manZ b1819 0.25 +1.6
1,902,131 + manZ b1819 0.25 -0.6
1,902,139 - manZ b1819 0.26 -0.0
1,902,139 - manZ b1819 0.26 +0.1
1,902,146 + manZ b1819 0.27 -3.1
1,902,425 - manZ b1819 0.59 -1.9
1,902,428 + manZ b1819 0.60 -0.1
1,902,618 + manZ b1819 0.82 +0.8
1,902,618 + manZ b1819 0.82 -1.1
1,902,626 - manZ b1819 0.83 +1.4
1,902,758 + -0.2
1,902,763 + -0.7
1,902,765 + -0.6
1,902,765 - -0.2
1,902,766 - +1.4
1,902,773 - +0.3
1,902,781 - -0.7
1,902,867 + -0.8
1,902,867 + -0.8
1,902,875 - yobD b1820 0.11 -0.0
1,902,875 - +2.0
1,902,875 - yobD b1820 0.11 -0.1
1,902,882 + yobD b1820 0.12 +0.5
1,902,893 + yobD b1820 0.15 -0.9
1,902,914 + yobD b1820 0.19 +0.1
1,902,964 + yobD b1820 0.30 -0.2
1,902,996 + yobD b1820 0.37 +0.1
1,903,006 - yobD b1820 0.39 -0.6
1,903,026 - yobD b1820 0.44 +1.0
1,903,040 + yobD b1820 0.47 +0.3
1,903,051 + yobD b1820 0.49 -2.6
1,903,051 + yobD b1820 0.49 -0.4
1,903,059 - yobD b1820 0.51 +1.4
1,903,069 - yobD b1820 0.53 +0.1
1,903,138 + yobD b1820 0.68 -1.3
1,903,161 + yobD b1820 0.73 -0.3
1,903,161 + yobD b1820 0.73 +1.5
1,903,179 - yobD b1820 0.77 +0.5
1,903,183 + yobD b1820 0.78 -0.2
1,903,196 + yobD b1820 0.81 -0.3
1,903,198 + yobD b1820 0.81 +0.6
1,903,496 - -0.2
1,903,498 - +0.3
1,903,529 + -0.0
1,903,537 - +0.4
1,903,548 + +0.3
1,903,553 + +1.1
1,903,558 + -0.9
1,903,558 + -0.4
1,903,583 + +0.1
1,903,602 - +0.1
1,903,602 - +0.1
1,903,639 - +0.2
1,903,654 - +0.0
1,903,702 + -2.1

Or see this region's nucleotide sequence