Strain Fitness in Escherichia coli BW25113 around b1207

Experiment: Guanosine 0.35306 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntychM and prsA are separated by 124 nucleotidesprsA and ispE are separated by 150 nucleotides b1206: ychM - orf, hypothetical protein (VIMSS), at 1,258,347 to 1,260,026 ychM b1207: prsA - ribose-phosphate pyrophosphokinase (NCBI), at 1,260,151 to 1,261,098 prsA b1208: ispE - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (NCBI), at 1,261,249 to 1,262,100 ispE Position (kb) 1260 1261 1262Strain fitness (log2 ratio) -2 -1 0 1 2at 1259.171 kb on - strand, within ychMat 1259.178 kb on - strand, within ychMat 1259.255 kb on - strand, within ychMat 1259.273 kb on + strand, within ychMat 1259.362 kb on - strand, within ychMat 1259.391 kb on - strand, within ychMat 1259.391 kb on - strand, within ychMat 1259.426 kb on - strand, within ychMat 1259.505 kb on - strand, within ychMat 1259.661 kb on + strand, within ychMat 1259.743 kb on + strand, within ychMat 1259.743 kb on + strand, within ychMat 1259.797 kb on + strand, within ychMat 1259.808 kb on + strand, within ychMat 1259.808 kb on + strand, within ychMat 1259.900 kb on + strandat 1259.943 kb on - strandat 1260.002 kb on - strandat 1260.002 kb on - strandat 1261.178 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Guanosine 0.35306 mM (C)
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1,259,171 - ychM b1206 0.49 -0.4
1,259,178 - ychM b1206 0.49 -0.6
1,259,255 - ychM b1206 0.54 +0.1
1,259,273 + ychM b1206 0.55 -1.2
1,259,362 - ychM b1206 0.60 -1.4
1,259,391 - ychM b1206 0.62 -0.2
1,259,391 - ychM b1206 0.62 -2.3
1,259,426 - ychM b1206 0.64 +2.0
1,259,505 - ychM b1206 0.69 -0.4
1,259,661 + ychM b1206 0.78 +0.5
1,259,743 + ychM b1206 0.83 +0.2
1,259,743 + ychM b1206 0.83 -2.1
1,259,797 + ychM b1206 0.86 -0.3
1,259,808 + ychM b1206 0.87 +1.1
1,259,808 + ychM b1206 0.87 -0.5
1,259,900 + +0.5
1,259,943 - +0.5
1,260,002 - +0.8
1,260,002 - +0.7
1,261,178 - -0.1

Or see this region's nucleotide sequence