Strain Fitness in Escherichia coli BW25113 around b1078

Experiment: Cytidine 0.41115 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgE and flgF are separated by 19 nucleotidesflgF and flgG are separated by 171 nucleotidesflgG and flgH are separated by 52 nucleotidesflgH and flgI are separated by 11 nucleotides b1076: flgE - flagellar hook protein E (NCBI), at 1,131,797 to 1,133,005 flgE b1077: flgF - flagellar component of cell-proximal portion of basal-body rod (NCBI), at 1,133,025 to 1,133,780 flgF b1078: flgG - flagellar component of cell-distal portion of basal-body rod (NCBI), at 1,133,952 to 1,134,734 flgG b1079: flgH - flagellar L-ring protein precursor H (NCBI), at 1,134,787 to 1,135,485 flgH b1080: flgI - homolog of Salmonella P-ring of flagella basal body (VIMSS), at 1,135,497 to 1,136,594 flgI Position (kb) 1133 1134 1135Strain fitness (log2 ratio) -1 0 1at 1132.979 kb on - strandat 1132.986 kb on - strandat 1132.998 kb on - strandat 1132.998 kb on - strandat 1133.002 kb on + strandat 1133.037 kb on + strandat 1133.162 kb on - strand, within flgFat 1133.162 kb on - strand, within flgFat 1133.215 kb on - strand, within flgFat 1133.251 kb on - strand, within flgFat 1133.251 kb on - strand, within flgFat 1133.286 kb on - strand, within flgFat 1133.318 kb on - strand, within flgFat 1133.324 kb on - strand, within flgFat 1133.336 kb on + strand, within flgFat 1133.367 kb on + strand, within flgFat 1133.389 kb on - strand, within flgFat 1133.417 kb on - strand, within flgFat 1133.467 kb on - strand, within flgFat 1133.477 kb on + strand, within flgFat 1133.610 kb on + strand, within flgFat 1133.618 kb on - strand, within flgFat 1133.833 kb on + strandat 1134.108 kb on + strand, within flgGat 1134.162 kb on + strand, within flgGat 1134.296 kb on - strand, within flgGat 1134.296 kb on - strand, within flgGat 1134.315 kb on - strand, within flgGat 1134.343 kb on - strand, within flgGat 1134.375 kb on + strand, within flgGat 1134.375 kb on + strand, within flgGat 1134.387 kb on + strand, within flgGat 1134.387 kb on + strand, within flgGat 1134.424 kb on + strand, within flgGat 1134.424 kb on + strand, within flgGat 1134.432 kb on - strand, within flgGat 1134.598 kb on - strand, within flgGat 1134.598 kb on - strand, within flgGat 1134.700 kb on - strandat 1134.752 kb on + strandat 1134.854 kb on - strandat 1134.857 kb on + strand, within flgHat 1134.893 kb on + strand, within flgHat 1134.893 kb on + strand, within flgHat 1134.901 kb on - strand, within flgHat 1135.012 kb on - strand, within flgHat 1135.101 kb on + strand, within flgHat 1135.257 kb on - strand, within flgHat 1135.634 kb on - strand, within flgI

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Per-strain Table

Position Strand Gene LocusTag Fraction Cytidine 0.41115 mM (C)
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1,132,979 - -0.3
1,132,986 - -0.4
1,132,998 - -0.0
1,132,998 - +0.7
1,133,002 + +0.0
1,133,037 + -0.4
1,133,162 - flgF b1077 0.18 +0.3
1,133,162 - flgF b1077 0.18 +0.1
1,133,215 - flgF b1077 0.25 +0.3
1,133,251 - flgF b1077 0.30 +1.2
1,133,251 - flgF b1077 0.30 +0.6
1,133,286 - flgF b1077 0.35 +0.3
1,133,318 - flgF b1077 0.39 +0.2
1,133,324 - flgF b1077 0.40 -0.2
1,133,336 + flgF b1077 0.41 -0.1
1,133,367 + flgF b1077 0.45 -1.5
1,133,389 - flgF b1077 0.48 +0.2
1,133,417 - flgF b1077 0.52 +0.6
1,133,467 - flgF b1077 0.58 +0.6
1,133,477 + flgF b1077 0.60 +0.1
1,133,610 + flgF b1077 0.77 +0.7
1,133,618 - flgF b1077 0.78 +0.2
1,133,833 + -0.7
1,134,108 + flgG b1078 0.20 -0.4
1,134,162 + flgG b1078 0.27 +0.1
1,134,296 - flgG b1078 0.44 +0.4
1,134,296 - flgG b1078 0.44 -0.4
1,134,315 - flgG b1078 0.46 -0.3
1,134,343 - flgG b1078 0.50 +0.2
1,134,375 + flgG b1078 0.54 -0.1
1,134,375 + flgG b1078 0.54 -0.3
1,134,387 + flgG b1078 0.56 -0.1
1,134,387 + flgG b1078 0.56 +0.3
1,134,424 + flgG b1078 0.60 +0.3
1,134,424 + flgG b1078 0.60 +0.6
1,134,432 - flgG b1078 0.61 -0.3
1,134,598 - flgG b1078 0.83 +0.2
1,134,598 - flgG b1078 0.83 -0.3
1,134,700 - -0.3
1,134,752 + -0.2
1,134,854 - -0.6
1,134,857 + flgH b1079 0.10 +0.4
1,134,893 + flgH b1079 0.15 +0.6
1,134,893 + flgH b1079 0.15 +1.0
1,134,901 - flgH b1079 0.16 -0.6
1,135,012 - flgH b1079 0.32 +0.2
1,135,101 + flgH b1079 0.45 +0.2
1,135,257 - flgH b1079 0.67 +0.4
1,135,634 - flgI b1080 0.12 +1.1

Or see this region's nucleotide sequence