Strain Fitness in Escherichia coli BW25113 around b1072

Experiment: TP4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmviN and flgN are separated by 39 nucleotidesflgN and flgM are separated by 4 nucleotidesflgM and flgA are separated by 75 nucleotidesflgA and flgB are separated by 154 nucleotidesflgB and flgC are separated by 3 nucleotidesflgC and flgD are separated by 11 nucleotides b1069: mviN - predicted inner membrane protein (NCBI), at 1,127,062 to 1,128,597 mviN b1070: flgN - export chaperone for FlgK and FlgL (NCBI), at 1,128,637 to 1,129,053 flgN b1071: flgM - anti-sigma factor for FliA (sigma 28) (NCBI), at 1,129,058 to 1,129,351 flgM b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086 flgA b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657 flgB b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065 flgC b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772 flgD Position (kb) 1129 1130 1131Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1128.553 kb on + strandat 1128.592 kb on + strandat 1128.600 kb on - strandat 1128.612 kb on + strandat 1128.932 kb on - strand, within flgNat 1129.057 kb on + strandat 1129.094 kb on - strand, within flgMat 1129.303 kb on + strand, within flgMat 1129.414 kb on - strandat 1129.433 kb on - strandat 1129.433 kb on - strandat 1129.579 kb on + strand, within flgAat 1129.663 kb on + strand, within flgAat 1129.716 kb on + strand, within flgAat 1129.771 kb on + strand, within flgAat 1129.771 kb on + strand, within flgAat 1129.810 kb on + strand, within flgAat 1129.811 kb on - strand, within flgAat 1129.813 kb on + strand, within flgAat 1129.815 kb on + strand, within flgAat 1129.815 kb on + strand, within flgAat 1129.958 kb on + strand, within flgAat 1130.045 kb on + strandat 1130.097 kb on + strandat 1130.110 kb on + strandat 1130.161 kb on + strandat 1130.236 kb on - strandat 1130.238 kb on - strandat 1130.238 kb on - strandat 1130.270 kb on - strandat 1130.352 kb on - strand, within flgBat 1130.352 kb on - strand, within flgBat 1130.411 kb on + strand, within flgBat 1130.411 kb on + strand, within flgBat 1130.456 kb on - strand, within flgBat 1130.456 kb on - strand, within flgBat 1130.513 kb on - strand, within flgBat 1130.570 kb on - strand, within flgBat 1130.693 kb on - strandat 1130.701 kb on + strandat 1131.014 kb on + strand, within flgCat 1131.014 kb on + strand, within flgCat 1131.054 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP4
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1,128,553 + +0.5
1,128,592 + +0.3
1,128,600 - +0.6
1,128,612 + -2.1
1,128,932 - flgN b1070 0.71 +0.3
1,129,057 + +0.1
1,129,094 - flgM b1071 0.12 -2.9
1,129,303 + flgM b1071 0.83 -0.4
1,129,414 - +0.5
1,129,433 - +0.6
1,129,433 - -0.0
1,129,579 + flgA b1072 0.23 -1.1
1,129,663 + flgA b1072 0.36 -0.4
1,129,716 + flgA b1072 0.44 -1.2
1,129,771 + flgA b1072 0.52 +0.1
1,129,771 + flgA b1072 0.52 +0.3
1,129,810 + flgA b1072 0.58 -0.6
1,129,811 - flgA b1072 0.58 -0.4
1,129,813 + flgA b1072 0.58 -1.2
1,129,815 + flgA b1072 0.59 -0.3
1,129,815 + flgA b1072 0.59 +0.5
1,129,958 + flgA b1072 0.80 -0.1
1,130,045 + +0.1
1,130,097 + +0.7
1,130,110 + -0.9
1,130,161 + +0.1
1,130,236 - +0.3
1,130,238 - -1.9
1,130,238 - +0.1
1,130,270 - -1.5
1,130,352 - flgB b1073 0.27 -0.3
1,130,352 - flgB b1073 0.27 -0.5
1,130,411 + flgB b1073 0.41 +0.0
1,130,411 + flgB b1073 0.41 -1.1
1,130,456 - flgB b1073 0.52 -1.7
1,130,456 - flgB b1073 0.52 -2.1
1,130,513 - flgB b1073 0.65 +0.3
1,130,570 - flgB b1073 0.79 +0.4
1,130,693 - +2.0
1,130,701 + +0.5
1,131,014 + flgC b1074 0.87 -3.3
1,131,014 + flgC b1074 0.87 -1.2
1,131,054 - +0.4

Or see this region's nucleotide sequence