Experiment: TP4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampC and frdD are separated by 62 nucleotides frdD and frdC are separated by 10 nucleotides frdC and frdB are separated by 10 nucleotides
b4150: ampC - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 4,375,834 to 4,376,967
ampC
b4151: frdD - fumarate reductase subunit D (NCBI), at 4,377,030 to 4,377,389
frdD
b4152: frdC - fumarate reductase subunit C (NCBI), at 4,377,400 to 4,377,795
frdC
b4153: frdB - fumarate reductase (anaerobic), Fe-S subunit (NCBI), at 4,377,806 to 4,378,540
frdB
Position (kb)
4377
4378 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4376.110 kb on + strand, within ampC at 4376.323 kb on - strand, within ampC at 4376.400 kb on - strand, within ampC at 4376.427 kb on + strand, within ampC at 4376.427 kb on + strand, within ampC at 4376.484 kb on + strand at 4376.493 kb on + strand, within ampC at 4376.541 kb on + strand, within ampC at 4376.541 kb on + strand, within ampC at 4376.581 kb on + strand, within ampC at 4376.594 kb on + strand, within ampC at 4376.596 kb on + strand, within ampC at 4376.634 kb on + strand, within ampC at 4376.642 kb on - strand, within ampC at 4376.690 kb on - strand, within ampC at 4376.690 kb on - strand, within ampC at 4376.693 kb on - strand, within ampC at 4376.794 kb on + strand, within ampC at 4376.794 kb on + strand, within ampC at 4376.800 kb on + strand, within ampC at 4376.982 kb on + strand at 4377.019 kb on - strand at 4377.043 kb on + strand at 4377.043 kb on + strand at 4377.078 kb on - strand, within frdD at 4377.078 kb on - strand, within frdD at 4377.126 kb on + strand, within frdD at 4377.126 kb on + strand, within frdD at 4377.155 kb on - strand, within frdD at 4377.155 kb on - strand, within frdD at 4377.320 kb on + strand, within frdD at 4377.320 kb on + strand, within frdD at 4377.401 kb on + strand at 4377.401 kb on + strand at 4377.401 kb on + strand at 4377.404 kb on - strand at 4377.404 kb on - strand at 4377.406 kb on - strand at 4377.409 kb on - strand at 4377.409 kb on - strand at 4377.409 kb on - strand at 4377.446 kb on - strand, within frdC at 4377.446 kb on - strand, within frdC at 4377.550 kb on + strand, within frdC at 4377.576 kb on + strand, within frdC at 4377.777 kb on + strand at 4377.794 kb on + strand at 4377.794 kb on + strand at 4377.812 kb on - strand at 4377.812 kb on - strand at 4377.812 kb on - strand at 4377.889 kb on + strand, within frdB at 4377.889 kb on - strand, within frdB at 4377.909 kb on - strand, within frdB at 4377.909 kb on - strand, within frdB at 4377.919 kb on + strand, within frdB at 4377.939 kb on - strand, within frdB at 4377.966 kb on - strand, within frdB at 4377.972 kb on + strand, within frdB at 4377.976 kb on + strand, within frdB at 4377.980 kb on - strand, within frdB at 4377.990 kb on - strand, within frdB at 4378.039 kb on + strand, within frdB at 4378.039 kb on + strand, within frdB at 4378.079 kb on - strand, within frdB at 4378.079 kb on - strand, within frdB at 4378.106 kb on - strand, within frdB at 4378.134 kb on + strand, within frdB at 4378.134 kb on + strand, within frdB at 4378.161 kb on + strand, within frdB at 4378.261 kb on - strand, within frdB at 4378.265 kb on - strand, within frdB at 4378.272 kb on + strand, within frdB at 4378.272 kb on + strand, within frdB at 4378.301 kb on + strand, within frdB
Per-strain Table
Position Strand Gene LocusTag Fraction TP4 remove 4,376,110 + ampC b4150 0.24 +1.0 4,376,323 - ampC b4150 0.43 +0.8 4,376,400 - ampC b4150 0.50 +0.7 4,376,427 + ampC b4150 0.52 +0.5 4,376,427 + ampC b4150 0.52 -2.0 4,376,484 + +1.5 4,376,493 + ampC b4150 0.58 -0.0 4,376,541 + ampC b4150 0.62 -2.2 4,376,541 + ampC b4150 0.62 -0.2 4,376,581 + ampC b4150 0.66 -0.1 4,376,594 + ampC b4150 0.67 +0.4 4,376,596 + ampC b4150 0.67 +0.2 4,376,634 + ampC b4150 0.71 -0.4 4,376,642 - ampC b4150 0.71 -0.8 4,376,690 - ampC b4150 0.75 +0.9 4,376,690 - ampC b4150 0.75 -0.1 4,376,693 - ampC b4150 0.76 +0.0 4,376,794 + ampC b4150 0.85 -1.6 4,376,794 + ampC b4150 0.85 -1.9 4,376,800 + ampC b4150 0.85 -0.8 4,376,982 + -1.3 4,377,019 - -0.7 4,377,043 + +0.3 4,377,043 + -1.3 4,377,078 - frdD b4151 0.13 -0.7 4,377,078 - frdD b4151 0.13 +2.3 4,377,126 + frdD b4151 0.27 -0.5 4,377,126 + frdD b4151 0.27 -1.6 4,377,155 - frdD b4151 0.35 -2.8 4,377,155 - frdD b4151 0.35 -0.6 4,377,320 + frdD b4151 0.81 -1.6 4,377,320 + frdD b4151 0.81 -2.0 4,377,401 + -1.4 4,377,401 + -0.0 4,377,401 + +0.7 4,377,404 - -1.7 4,377,404 - -1.1 4,377,406 - +0.5 4,377,409 - -0.0 4,377,409 - +0.5 4,377,409 - +0.2 4,377,446 - frdC b4152 0.12 -0.3 4,377,446 - frdC b4152 0.12 -0.6 4,377,550 + frdC b4152 0.38 -1.7 4,377,576 + frdC b4152 0.44 +2.1 4,377,777 + -0.7 4,377,794 + -1.4 4,377,794 + +0.1 4,377,812 - +1.0 4,377,812 - -1.2 4,377,812 - -0.6 4,377,889 + frdB b4153 0.11 -0.5 4,377,889 - frdB b4153 0.11 -2.4 4,377,909 - frdB b4153 0.14 +0.6 4,377,909 - frdB b4153 0.14 -1.7 4,377,919 + frdB b4153 0.15 -0.2 4,377,939 - frdB b4153 0.18 -0.4 4,377,966 - frdB b4153 0.22 -0.0 4,377,972 + frdB b4153 0.23 -2.3 4,377,976 + frdB b4153 0.23 -0.9 4,377,980 - frdB b4153 0.24 -0.8 4,377,990 - frdB b4153 0.25 +1.3 4,378,039 + frdB b4153 0.32 +0.9 4,378,039 + frdB b4153 0.32 +1.0 4,378,079 - frdB b4153 0.37 -2.6 4,378,079 - frdB b4153 0.37 -0.1 4,378,106 - frdB b4153 0.41 -1.4 4,378,134 + frdB b4153 0.45 -0.0 4,378,134 + frdB b4153 0.45 +0.2 4,378,161 + frdB b4153 0.48 -0.2 4,378,261 - frdB b4153 0.62 -1.6 4,378,265 - frdB b4153 0.62 -2.9 4,378,272 + frdB b4153 0.63 -0.7 4,378,272 + frdB b4153 0.63 +0.3 4,378,301 + frdB b4153 0.67 -1.1
Or see this region's nucleotide sequence